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Citations for Promega :
6251 - 6300 of 6889 citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... beads were suspended in digestion buffer (Tris 50 mM pH 7.5, urea 2 M, 1 mM DTT and 5 µg.µl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of each primer (10 μM) and 12.5 μL of GoTaq Green Master mix (Promega®, USA). The PCR was run in a thermocycler (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... dissolved in 2 volumes of 0.1 M Tris-HCl (pH 8.0) with the addition of 1 μg of trypsin (Sequencing Grade Modified, Promega) and incubated for 12 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... After that pancreatic enzyme was added at a ratio of 1:50 (Trypsin Gold, Promega, Fitchburg, WI, USA), and incubated overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:4 with 50 mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 µL of 50 mM ammbic (pH 8.3 ...
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bioRxiv - Microbiology 2023Quote: ... AePer50 reverse transcription (RT) and qPCR reactions were performed using GoTaq® 1-Step RT-qPCR System (Promega), with 10 µl of 2X qPCR Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with anti-rabbit horseradish peroxidase (HRP) conjugate antibodies (1:20000 dilution) (Promega, Madison, WI, USA) for two hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... A 90 μl volume of each culture and 10 μl of the furimazine (1 in 200 dilution; Promega) were incubated in a Cellstar non-transparent white 96-well microplate for one h and the bioluminescence intensity was monitored with a Tecan Infinite 200 PRO plate reader every 30 s ...
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bioRxiv - Bioengineering 2024Quote: The primary antibodies used in this study were the following: rb-α-Nanoluc (non-commercial, Promega, 1:1000); rb-α-CD63 (ab68418 ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were incubated with 80 μL of 2 M urea in 50 mM Tris-HCl (pH 7.5) containing 1 mM DTT and 0.5 μg trypsin (Promega) at 25°C with shaking for pre-digestion ...
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bioRxiv - Developmental Biology 2024Quote: ... From there on all buffers were supplemented with 1:100 RNasin Plus RNase inhibitor (Promega, Cat. No. N2615).
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bioRxiv - Cancer Biology 2024Quote: ... G17-1 cell proliferation was measured by MTS assay (Cell Titer 96 Aqueous One, Promega, Madison, WI, USA).
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bioRxiv - Synthetic Biology 2024Quote: ... pre-ran reaction solutions were incubated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Genomics 2019Quote: mRNA was isolated from frozen samples using the Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simplyRNA Tissue Kit (both Promega Corporation, Madison, WI, USA). The mRNA was isolated according to protocol with a modified lysis step as described in Snoek et al ...
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bioRxiv - Bioengineering 2022Quote: ... cells were lysed with 60 μL of either Total Glutathione Lysis Reagent or Oxidized Glutathione Lysis Reagent (provided in Promega GSH/GSSG-Glo Assay kit, V6611). After lysis ...
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bioRxiv - Zoology 2023Quote: ... Skeletal architecture was examined by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20 mg/ml Proteinase K (Promega) ...
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bioRxiv - Zoology 2022Quote: ... Skeletal architecture was investigated by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Pathology 2022Quote: ... The infectious BSMV RNAs were transcribed in vitro from linearized plasmids γ-TaPDS, γ-TaCBS, γ-TaGSTU6, γ, α, and β (Petty et al., 1989) using the RiboMAX Large Scale RNA Production System-T7 kit (Promega, USA) and the Ribo m7G Cap Analog (Promega) ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg total RNA using the M-MLV Reverse Transcriptase (Promega, Cat. #M1705) (primers are listed in Supplementary Table 8) ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested vector (200 ng) was ligated with 1 µL of the oligonucleotides (0.45nmol/μl) using T4 DNA ligase (Promega) for 3 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was quantified to 3 μg to react 1 μg/μL random hexamer (C1181; Promega, Madison, WI, USA) at 70°C for 5 min ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then digested with mass-spectrometry grade trypsin (1:20, Trypsin:Substrate, Promega Gold Trypsin, San Luis Obispo, CA) overnight at 37°C [66] ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Plant Biology 2019Quote: ... Secondary goat anti-rabbit IgG conjugated with HRP was used at a dilution of 1:10,000 (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2019Quote: ... The sample was then diluted 50% with PBS before sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) was added for an overnight incubation at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... The samples were diluted to 1% SDC using 100mM ammonium bicarbonate and mass spectrometry grade Trypsin (Promega, WI, USA) was added at a ratio of 1:50 (µg Trypsin:µg Protein ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of forward and reverse primer (5 μM, see Supplementary Table 1) and 5.5 μL of Nuclease-free Water (Promega). The PCR I was performed as follows ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from sorted cells with RiboEx (GeneAll) and 0.2–1 μg was subjected to cDNA synthesis using the GoScript Reverse Transcription system (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The 1/10 of the sample was kept for injection and the 9/10 were digested with trypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Postnatal testes chemically synchronized for undifferentiated spermatogonia were homogenized at 2% w/v in polysome buffer (50 mM Tris, pH 7.4, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1mM DTT, 200 U/mL Promega RNasin ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1 cells were co-transfected with 1 µg KIF1C-mCherry-FRB and 0.5 µg FKBP-GFP-Myc-MAO using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Systems Biology 2021Quote: ... Then urea concentration was diluted to 1.5 M with HPLC water and 1:50 (w/w) trypsin (Promega GmbH) was added for 12 h at 37 °C with mixing at 700 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: ... The translation reaction mixture for single-molecule detection was assembled according to the manufacturer’s instructions with the following modifications: 1) the RNase inhibitor RNaseIn (Promega) was used at a final concentration of 0.8 U/ul ...
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bioRxiv - Microbiology 2021Quote: ... Protein was reduced with 5 mM DTT and alkylated with 14 mM iodoacetamide followed by digestion with trypsin (1:100, w:w) (Promega) as described in [80] ...
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bioRxiv - Microbiology 2021Quote: ... 0.1 % Tween 20 and incubated for 1 h with the secondary IgG (HRP-conjugated donkey anti-rabbit antibodies, Promega) diluted 1:20,000 in TBS ...
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bioRxiv - Cell Biology 2021Quote: ... For Halo-tag labeling cells were allowed to re-attach for 1 hour under hypoxic conditions and then incubated with 0.5 uM of Janelia Fluor 549 (Promega) for 15 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...