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Citations for Promega :
551 - 600 of 4197 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Core Kit (Promega, Wisconsin, USA), following the manufacturer’s protocol ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: Proteins were digested overnight at 37°C in 20 ml of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 mg/ml; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile and 0.1% formic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega) at 4°C before incubation overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... were added to the 5′ end labelled 187cspA RNA in a total volume of 10 µL in Buffer O containing 0.4 U/µL of RNasin (Promega). Complex formation was performed at 15°C or 37°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated at either 15°C or 37°C for 10 min in Buffer I containing 0.4 U/µL of RNasin (Promega). Following renaturation ...
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bioRxiv - Immunology 2019Quote: ... and the pellet was resuspended in 20 mL buffer A (NaPO4 20 mM, NaCl 500 mM and imidazole 20 mM, pH 7,4) containing 5 U of DNAse (Promega) and 30 ug/mL lysozyme (Sigma ...
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bioRxiv - Systems Biology 2019Quote: ... and 20 μL of diluted insect cell extract (containing 4 μL of ICE, 3.2 μL of 5x ICB dilution buffer and 8 units of T7-RNAP-Promega#P4074 ...
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bioRxiv - Biochemistry 2019Quote: ... 70 000-100 000 cells (in 200-450 μl DMEM) were seeded into each well containing a transfection cocktail of 0.4 μl of Fugene 6 (E2693, Promega), with 20 μl Opti-MEM (11058-021 ...
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bioRxiv - Plant Biology 2019Quote: ... PCR reactions with 50 ng plasmid template were performed in 50 µl volume containing 10x DreamTaq buffer and 2.5 units of DreamTaq polymerase (Promega). Standard PCR conditions were 94 °C for 5 min ...
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bioRxiv - Biophysics 2020Quote: ... Gel plugs were soaked in 50 mM ammonium bicarbonate (pH 7.8) containing 20 μg/mL of trypsin gold (Promega) for 24 hours at 37°C ...
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bioRxiv - Genomics 2021Quote: ... The chromatin was then incubated for 30 min at 37° C in 50 µl DNase I solution (1X DNase I buffer containing 0.2 U/µl DNase I [Cat #M6101, Promega] and 0.25 U/µl Protector RNase Inhibitor) ...
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bioRxiv - Immunology 2021Quote: ... Single CD3-CD8-CD14-CD16-CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Cell Biology 2019Quote: ... parental HeLa cells were transfected with 2 μg plasmid containing a GFP cassette and 4 μl FuGENE HD (Promega) in a six-well cluster ...
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bioRxiv - Systems Biology 2020Quote: Frozen fly brains were resuspended in 100 μL of lysis solution containing 0.1% (w/v) ProteaseMAX Surfactant (Promega, USA), 50 mM ammonium bicarbonate ...
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bioRxiv - Neuroscience 2020Quote: cDNA synthesis was performed in a reaction volume of 25 μl (10 cell pools) or 20 μl (single cells) containing dNTPs (0.5 mM, Promega), random primers (100 ng/per tube ...
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bioRxiv - Cell Biology 2021Quote: ... Each PCR reaction was performed in a 10 μL mixture containing 0.25 U GoTaq G2 Hot Start Polymerase (Promega), 1x GoTaq G2 Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... The psiCheck2 reporter construct containing dual Renilla and Firefly luciferase reporters was purchased from Promega (Promega, Madison, WI, USA). The DDX5-bound sequences identified by eCLIP IDR were cloned into a multiple cloning site located downstream of the Renilla translational stop codon ...
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bioRxiv - Cell Biology 2021Quote: ... collected at 2000 x g followed by resuspension in 0.5 ml of storage buffer (PBS containing 4% BSA and RNasin (Promega) – 0.2 U/ul ...
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bioRxiv - Biochemistry 2020Quote: ... The gel pieces were dried in a vacuum centrifuge then rehydrated in 50 mM ABC containing 12.5 ng/μL trypsin (Promega), 5 mM calcium chloride ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 200 µl of 25 mM ABC containing 500 ng of sequencing grade trypsin (Promega, catalog # V5111) and incubated for 16 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
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bioRxiv - Plant Biology 2021Quote: ... On bead digestion was performed in 50 µl of ammonium bicarbonate solution containing 4 µl of Trypsin/LysC (Promega) solution and incubated at 37°C for 3 h in a thermomixer at 1,000 RPM ...
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bioRxiv - Microbiology 2020Quote: ... The subsequent amplifications were performed using five microliters of dC-tailed cDNA in 50 μl final volume containing 2.5 units of GoTaq polymerase (Promega), 200 μM of each dNTP ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were washed three times with TBS buffer containing 0.1% Tween 20 and finally the membranes were developed with BCIP/NBT color development substrate (Promega) to enable visualization of protein bands (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... First the crosslinked RNA was dephosphorylated using 0.1U/μL FastAP (Thermofischer) in Reaction buffer containing 1U/μL RNAsin (Promega) for 45min at 37°C and stopped by washing once with Denaturing buffer and three times with Reaction buffer supplemented with 0.5% NP-40 ...
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bioRxiv - Microbiology 2020Quote: ... with the required amount of cells (cell number equal to the number of wells multiplied by 1.5 × 104).The effector and target cells were mixed 20 h post-transfection at 37°C in fresh medium containing 60μMmembrane-permeable substrate for Renilla luciferase (RL) (Enduren Live Cell Substrate; Promega)1 h prior to mixing ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with linearized repair templates and the PX459 plasmid containing the appropriate guides using FuGENE HD (Promega) with a ratio of Reagent ...
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bioRxiv - Cell Biology 2022Quote: ... Liquid was removed and gel pieces were dehydrated in 100% ACN at RT for 5 min at 2,000 rpm followed by rehydration in 100 mM NH4HCO3 containing 14 ng/μL trypsin (Promega) on ice for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... The multiplex PCR was performed in a 12.5 μl volume containing 2.5 μL of 5x GoTaq® Reaction Buffer (Promega), 0.25 μL of dNTP (10 mM) ...
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bioRxiv - Microbiology 2022Quote: Each colony PCR consisted of a 25-μl reaction containing: 12.5 μl 2x GoTaq Green Master mix (Promega, UK), 0.5 μl of the reverse and forward primers (10 μM each) ...
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bioRxiv - Cancer Biology 2022Quote: ... before the addition of digestion buffer (50 mm Tris) containing 0.8 μg Lys-C (Wako, 129–02541) and 0.8 μg Trypsin-gold (Promega, V5280). Enzymatic digestions proceeded under agitation (400 rpm ...
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bioRxiv - Immunology 2022Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−Gamma NTD-PE+Wuhan-Hu-1 NTD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Microbiology 2022Quote: ... 40 μL of the cells’ supernatant containing secreted GLuc were mixed with Renilla luciferase Assay Reagent (100 μl) (Promega). GLuc activity was measured using SpectraMax i3 Multi-mode Detection Platform (Molecular Devices).
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Neuroscience 2022Quote: ... and placed in 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... 2 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Genomics 2022Quote: ... Transfection efficiencies were determined in parallel by preparing transfection mixes containing 4μL FuGENE HD transfection reagent (Promega, Cat#: E2311), 96μL Opti-MEM (Life Technologies ...
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bioRxiv - Epidemiology 2019Quote: ... The 16S rDNA touchdown PCR was performed in 20 µL reactions containing 1× GoTaq® Green Mastermix (Promega, USA), 0.4 µM of each primer SpirRNAF and SpirRNAR (S2 Table ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplifications were carried out in a total reaction volume of 10μL containing 5 μL of 2xGoTaq master mix (Promega) and 2.5 μL of 4M betaine ...
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bioRxiv - Developmental Biology 2020Quote: ... A BAC clone containing exon 14 of the trp53bp2 gene was subcloned into a ∼2.4kb backbone vector (pSP72, Promega) containing an ampicillin selection cassette for retransformation of the construct prior to electroporation ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions with 50 ng plasmid template were performed in 50 µl volume containing 10x DreamTaq buffer and 2.5 units of DreamTaq polymerase (Promega). Standard PCR conditions were 94 °C for 5 min ...
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bioRxiv - Genetics 2019Quote: ... The vectors containing the gRNA sequence and the donor template were co-transfected using FuGENE HD transfection reagent (Promega). GFP +ve cells were selected by FACS 48 h after transfection and plated at clonal density ...
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bioRxiv - Biophysics 2019Quote: ... the fluid chambers were exposed to a PBS pH 7.4 solution containing 1% glutaraldehyde to subsequently immobilize the reference beads (Spherotec) and the HaloTag ligand (Promega).
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bioRxiv - Genomics 2019Quote: ... We cloned sequences containing reference alleles in the forward orientation upstream of the minimal promoter of firefly luciferase vector pGL4.23 (Promega) using KpnI and SacI restriction sites ...
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bioRxiv - Cell Biology 2019Quote: Proteins were then digested overnight at 37°C in 20 μL of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 μg/mL; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile ...
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bioRxiv - Physiology 2019Quote: ... ~80mg of tissue is first placed in 1.5 mL microcentrifuge tubes containing 250 μL of 1x passive lysis buffer (Promega) and homogenized ...
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bioRxiv - Genetics 2021Quote: ... we cloned human DNA sequences (Coriell) containing the reference or alternate allele upstream of the minimal promoter in the luciferase reporter vector pGL4.23 (Promega) in the forward direction using the restriction enzymes SacI and KpnI ...