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Citations for Promega :
451 - 500 of 4197 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a 30 µL reaction volume containing 1X Colorless GoTaq Reaction Buffer (Promega, #M792B), 200 µM dNTPs (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... synthetic gene fragments containing response elements were inserted into a pGL4.23[luc2/minP] vector obtained from Promega using KpnI and HindIII (for SRE ...
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bioRxiv - Cell Biology 2023Quote: ... sections were incubated for one hour at room temperature in PBS containing 0.05% Tween 20 (Promega, H5152) and 5% non-fat dry milk as a blocking agent ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
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bioRxiv - Systems Biology 2024Quote: ... followed by 80μL Hank’s balanced salt solution (HBSS) containing 10mM HEPES and 10μM furimazine/NanoGlo Luciferase Assay Substrate (Promega). BRET measurements were performed at 37 °C using a PHERAstar Microplate Reader (BMG Labtech ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing rsbU or rsbT/rsbUY28I were subjected to PCR mutagenesis using GoTaq DNA polymerase mix (Promega) supplemented with 2mM additional MgCl2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of LHB promoter-containing reporter vector were transfected with 5 ng of pRLRenilla (#E2231, Promega), with or without 50 ng of pCMV6-NR2F2-WT or p.Arg246His and/or 5 ng of pCMX-NR5A1 ...
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bioRxiv - Microbiology 2024Quote: ... Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade) was added and the traps were incubated 2h at 47°C in a covered thermomixer without vortexing ...
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bioRxiv - Cell Biology 2020Quote: ... As secondary antibodies we used horseradish peroxidase coupled anti-mouse (Promega W402B), anti-rabbit (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used are mouse β-Gal (1:250; Promega #Z3781), mouse anti-Wingless (1:100 ...
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bioRxiv - Biophysics 2019Quote: ... the horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (Promega, W4021) and a Goat anti-Rabbit IgG (H&L) ...
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bioRxiv - Immunology 2019Quote: ... HRP-conjugated antibodies (anti-mouse or anti-rabbit IgG HRP conjugate, Promega) were detected by enhanced chemiluminescence detections (Thermofisher ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or HA-mouse μOR and pGloSensor22F-cAMP plasmids (Promega, Madison, WI, USA) using Xtremegene9 (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... polyclonal anti-mouse IgG-HRP raised in goat (W402B, 1:3,000; Promega) and polyclonal anti-rabbit IgG-HRP raised in goat (R1364HRP ...
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bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
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bioRxiv - Microbiology 2020Quote: ... Secondary HRP conjugated goat anti-mouse IgG antibodies (Promega, Madison, WI; W4021) was used at 1:50,000.
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega). Proteins were visualized using chemiluminescence detection reagent (ECL ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: mouse anti-beta-galactosidase (Promega; 1:100), mouse anti-Mmp1 (1:1:1 mixture of DSHB 3A6B4 ...
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bioRxiv - Molecular Biology 2022Quote: ... Both mouse brain and cell lysates were digested with DNase I (Promega) for 5 minutes at 37°C and then with RNase A (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... Goat immunoglobulins against rabbit or mouse immunoglobulins conjugated with horseradish peroxidase (Promega) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... HRP conjugated anti-rabbit (W4011) and anti-mouse (W4021) were from Promega and used at a dilution of 1:10000 ...
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bioRxiv - Biophysics 2022Quote: ... and a goat anti-mouse tag: HRP 0.1 μL/mL (W402B - Promega).
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bioRxiv - Genetics 2023Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies (Promega) were used at 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... secondary antibodies: anti-rabbit HRP or anti-mouse HRP (Promega, 1:10000).
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bioRxiv - Cell Biology 2024Quote: ... followed by HRP-conjugated secondary anti-mouse antibody (1:5000, Promega W402B), diluted as indicated ...
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bioRxiv - Cell Biology 2020Quote: ... caspase8 Kit (Promega company); Caspase3 / 7 Kit (Promega company).
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bioRxiv - Molecular Biology 2020Quote: ... or Maxwell kit (Promega). Following RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... the GoScript Kit (Promega) was used to make cDNA and qRT-PCR performed on a BioRAD c1000 Touch thermocycler.
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bioRxiv - Cell Biology 2019Quote: Proteins were incubated overnight at 37°C with 20 µl of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5µg/ml, Promega) at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were resuspended in 50 μL 50 mM TEAB pH 8.5 containing 0.1% Na-deoxycholate and 200 ng of trypsin (Promega), before incubation overnight at 37 °C with periodic shaking (Eppendorf Thermomixer) ...
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bioRxiv - Microbiology 2019Quote: ... swab tips were placed into DNA/DNase/RNase-free 1.5 ml Eppendorf tubes containing nuclease-free water (Promega). The swab shafts were cut with a pair of sterile dissection scissors to allow closing of the tubes ...
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bioRxiv - Cell Biology 2019Quote: ... Gel pieces were rehydrated in 10-15 µL of solution containing 20 ng/µL trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate for 15 min ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... the reactions were carried out in triplicates in a reaction mixture containing 1x GoTaq qPCR MasterMix (Promega Corporation), 300 nM CXR Reference Dye ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega). The reaction was carried out at 42°C for 50 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega) in a total reaction volume of 25 μl ...
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bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were co-transfected with luciferase-containing constructs (pGL3) and phRL-TK synthetic renilla vector (Promega, Madison, WI) in a molar ratio of 1:60 (phRL-TK versus pGL3) ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid pGL4.32 containing a firefly luciferase cassette under NF-κB response elements was obtained from Promega (Cat# E8491). For overexpression of ubiquitin ...
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bioRxiv - Cell Biology 2019Quote: ... Gel slabs were then re-hydrated using 50 mM ammonium bicarbonate pH 8.4 (containing 0.04 μg sequencing grade modified porcine trypsin (Promega)) and incubated for 16 h at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... In-column digest was performed by adding 25 µL of a solution containing 2 µg trypsin (Promega, V5111) in 50 mM TEAB pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... and rehydrated with 50 mM ammonium bicarbonate solution containing 12.5 ng/µL of sequencing-grade modified trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Biophysics 2019Quote: ... the beads were incubated in a PBS solution containing 25 μg/mL of the HaloTag ligand O4 (Promega) for at least 4 h at constant rotation (room temperature) ...
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bioRxiv - Molecular Biology 2020Quote: ... The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega). The samples were incubated for 30min at 22°C prior to magnetic capture of the protein-RNA complex ...
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bioRxiv - Developmental Biology 2019Quote: The PCR mix was generated by adding 2µl of the lysed solution to a solution containing 1.25µl GoTaq™Flexy polymerase (Promega), 0.31 mM dNTPs (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
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bioRxiv - Neuroscience 2021Quote: ... gel pieces were rehydrated with 10 µL 50 mM NH4HCO3 containing 25 µg/mL trypsin (Trypsin Gold, Promega) and incubated for 15 min on ice ...
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bioRxiv - Genetics 2020Quote: ... The 10 µL Zn-finger PCR reaction was prepared containing of 2 µL of 5X reaction buffer (Promega), 0.8 µL of 2.0 mM MgCl2 ...