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Citations for Promega :
551 - 600 of 1206 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Cloning of The PCR products were performed in the vector pGEM®-T (Promega) containing the lactose operon and the gene coding for ampicillin resistance and then transformed into chemocompetent bacteria (DH5 ...
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bioRxiv - Plant Biology 2019Quote: ... We ligated the amplicons into the pGEM-T easy vector (Promega, Madison, WI, USA) by blunt end cloning according to the manufacturer’s instructions and sequenced the inserts ...
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bioRxiv - Developmental Biology 2021Quote: ... The gel-purified fragment was cloned into the p-GEM-T Easy vector (Promega) and digoxigenin-labelled probes generated by in vitro transcription using a DIG RNA labelling kit (Roche).
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bioRxiv - Genetics 2021Quote: ... The resulting fragments were purified and cloned into pGEM®-T Easy Vector (Promega). Ligations and transformations were performed as directed by the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... uncleaved band was sub-cloned into pGEM®-T Easy Vector (Promega Corporation, A1360) and the insertions and deletions (indels ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR product was cloned into the pGEM-T Easy vector system (Promega) and then digested with the restriction enzymes ...
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bioRxiv - Cancer Biology 2020Quote: ... in-gel purified and ligated into the pGEM-T Easy vector (Promega, Cat. A1360) and submitted to Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were purified and cloned into a pGEM-T Easy vector (Promega, A1360) according to the manufacturer’s instructions and the identity of inserts confirmed by sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was cloned into the pGEM-T vector (Promega, Madison, WI, USA) and confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Each product was cloned using the pGEM®-T Easy vector kit (Promega Corp.) to derive 12 clones using the GeneJet plasmid purification kit (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Gel extracted products were ligated into the pGEM-T Easy Vector System I (Promega). Ligation products were transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... The amplified fragment cloned into pGEM-T Easy vector (Promega K. K., Tokyo, Japan) was used to synthesize DIG-labeled sense and antisense RNA probes using DIG RNA labeling kit (SP6/T7 ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments from the nested PCR were cloned into pGEM-T-easy vectors (Promega), and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
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The arms race in noncoding regions of transposable element: the evolution of anti-silencing and RNAibioRxiv - Genetics 2022Quote: ... amplified DNA was purified and cloned into the pGEM T-Easy Vector System (Promega). At least 14 copies were sequenced for each sample ...
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bioRxiv - Biochemistry 2023Quote: ... The amplified fragment was subcloned in pGEM®-T Easy (Promega, Madison, WI, US) and sequenced (Genwiz ...
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bioRxiv - Cancer Biology 2023Quote: ... The amplified PCR products were isolated and subcloned into pGEM-T easy vector (Promega) and sequenced using Bigdye terminator v3.1 (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR product was cloned using the pGEM®-T Easy vector (Promega, USA) before the sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR-amplified products were then cloned into the pGEM-T Easy Vector (Promega) and subjected to sequencing for further analysis.
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bioRxiv - Microbiology 2020Quote: ... 50 µ L of Kinase-Glo® reagent (Promega) was then added and incubated at room temperature for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... or goat anti-Human IgG (H+L) (Promega, W4031) antibodies were added for 30 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... digested using 20 ng/ l sequencing grade trypsin (Promega) for 1 hr at 47C ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Immunology 2022Quote: ... the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison, WI) was used to isolate viral RNA (vRNA ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extracts were treated using RQ1 RNAse-free DNAse (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a complete standard mixture of amino acids (Promega, Medison, WI, USA) was analyzed by CE-MS to obtain MS and MS/MS spectra of the proteinogenic amino acids ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently stained with Diamond™ Nucleic Acid Dye (Promega, H1181) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... B.1.351) with TMPRSS2 into 293T cells using Fugene 6 transfection reagent (Promega, Madison, WI)54–56 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: N-terminal NanoLuc/Kinase fusions were encoded in pFN31K or pFC32K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
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bioRxiv - Biochemistry 2022Quote: ... N-or C-terminal NanoLuc/Kinase fusions were encoded in pFN31K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
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bioRxiv - Biophysics 2021Quote: ... The primer sequence of SmBiT was designed from pBiT2.1-N [TK/SmBiT] Vector (Promega).
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bioRxiv - Biochemistry 2023Quote: N-terminal large (LgBit) and C-terminal small (SmBit) fragments derived from NanoLuc (Promega) were fused to the C-terminus of B56δ and inserted downstream of its N-arm at residue 103 ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
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bioRxiv - Neuroscience 2023Quote: ... eluted in-solution samples were alkylated with N-Ethylmaleimide and digested with trypsin (Promega) using micro S-trap column (ProtiFi) ...
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... were made using the NanoBRET™ PPI MCS Starter System (Promega, cat n° N1811). The RRM2 fragments were amplified by PCR using Phusion polymerase (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Electroporated Halo-CENP-T was labelled using the Halo Tag Oregon Green fluorescent ligand (Promega) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2019Quote: ... The probe for PHB detection was cloned into the pGEM-T Easy vector (Promega, USA), using the primers listed in Table S2 ...
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bioRxiv - Cancer Biology 2020Quote: ... from the 1.5% TAE gel and cloned into the pGEM- T Easy Vector System (Promega) for transformation ...
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bioRxiv - Genetics 2019Quote: ... which was cloned using the pGEM®-T Easy Vector System (Promega, Madison, WI, USA), and the resulting clone sequenced (GENBANK accession no ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were cloned into pGEM®-T Easy vector (Promega, cat. no: A1360), and ten colonies were selected for sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... and were then cloned using a pGEM→-T Easy Vector System (Promega, cat no: A1380). For each sample ...
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bioRxiv - Genetics 2019Quote: ... The resulting PCR products were inserted by TA cloning into pGEM-T Easy plasmid (Promega) possessing T7 and SP6 promoters ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified DNA was subcloned into a TA cloning vector pGEM-T (Promega, Madison, WI) using TaKaRa Ex Taq polymerase (Takara Bio ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified products were ligated into the pGEM-T easy vector (Promega, Madison, WI, USA) by blunt end cloning according to the manufacturer’s instructions and sequenced ...
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bioRxiv - Neuroscience 2020Quote: A probe of HarmOR13 was synthesized from linearized pGEM-T vector (Promega, Madison, WI, USA). Digoxigenin (DIG)-labeled probes were synthesized with DIG RNA labeling kit version 12 (SP6/T7 ...
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bioRxiv - Plant Biology 2020Quote: ... The resultant DNA was inserted into the pGEM-T Easy vector (Promega, Madison, WI, USA). The recombinant plasmid DNA was then used to obtain pGEM-cmaA as templates ...
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bioRxiv - Microbiology 2020Quote: ... These fusions were expressed either from a plasmid based on the pGEM-T backbone (Promega) named pOP plasmid (Daskalov et al ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were cloned into pGEM-T Easy and transformed into DH5α competent cells (Promega). Plasmid DNA was purified using the Qiagen midiprep kit (Qiagne ...
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bioRxiv - Microbiology 2021Quote: ... Purified PCR products were cloned into pGEM®-T Easy Vector (Promega, Madison, WI, USA), and transformed into OneShot TOP10 chemically competent Escherichia coli cells (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Each of the resultant cDNA was cloned into the pGEM-T Easy vector (A1360, Promega) for the generation of cDNA templates ...