Labshake search
Citations for Promega :
551 - 600 of 653 citations for D Galactose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Nuclei were washed 3 times by resuspending in cold PBS containing 2% fraction V bovine serum albumin and 0.2 U / µl RNase inhibitor (Promega, N2615). For multiome assay ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
-
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
-
bioRxiv - Microbiology 2023Quote: ... and then reverse transcribed into complementary DNA (cDNA) using 30 enzyme units(U) of the MMLV-RT (Promega, WI, USA) and 1μM of the labeled primer ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma], 20 U ml-1 RNase inhibitor [Promega] ...
-
bioRxiv - Cell Biology 2022Quote: ... The slides were exposed to 365 nm UV light and the damaged BrdU/BrdC-substituted DNA strands were subsequently digested by 800 U Exonuclease III (Promega) in the dedicated buffer (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Developmental Biology 2023Quote: ... The synthesis of cDNA was achieved by incubation in a 20 µl reaction mixture containing 200 U of MMLV Reverse Transcriptase (Promega) and 500 ng of random primer (Roche ...
-
bioRxiv - Systems Biology 2023Quote: ... The panel of ssDNA-labelled antibodies (250 ng/ml for each antibody) was mixed with 2 U/µl RNAsin Plus (Promega) and 0.1% Triton X-100 in PBS:PFBB (1:1 ...
-
bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
-
bioRxiv - Neuroscience 2024Quote: Nuclear fractions from Con (n=13) and DLB (n=12) were pretreated with DNAse (50 u/ml, 15 mins RT, RQ1 DNAse, Promega), were adjusted to 80 ng /µl with LDS sample buffer (Fisher Scientific) ...
-
bioRxiv - Neuroscience 2024Quote: ... Approximately 0.5µg of RNA from each sample were reverse-transcribed to become cDNA by mixing with the master-mix (200 u/µL M-MLV Reverse Transcriptase, Promega Cat ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice in nuclei wash and resuspension buffer consisting of PBS with 0.1% BSA and 0.2 U/μl RNase inhibitor (#N2615, Promega, Madison, WI). Cells were passed through a 50 μm CellTricsTM filter (#NC9491906 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The synthesis of cDNA was achieved by incubation in a 20 μl reaction mixture containing 200 U of MMLV Reverse Transcriptase (Promega) and 500 ng of random primer (Roche ...
-
bioRxiv - Neuroscience 2024Quote: ... Dissected tissue was homogenized in lysis buffer (10% Triton X-100, 0.1 M DTT, 40 U/μL RNasin (Promega, N2115), protease inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... into 96-well PCR plates with 1 μL of cell lysis solution (1:10 Cell Lysis buffer [Roche], 10 U/μL Rnasin plus Ribonuclease inhibitor [Promega]) in each well ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10 mM NaCl, 3 mM MgCl2, 1% BSA, 0.1% Tween-20, 1 mM DTT and 1 U/uL RnaseIn, Promega©) was added to the lysed cells ...
-
bioRxiv - Plant Biology 2021Quote: ... For multiple day measurements plants were sprayed daily with 1 mM D-luciferin (Promega, Fitchburg, Wl, USA) at 10 am ...
-
bioRxiv - Synthetic Biology 2021Quote: 1μl of 50μM oligo d(T)20 (IDT) and 1μl of 10mM dNTP mix (Promega cat #U1240) were combined with 1 μg of RNA before adding dd H20 up to a final volume of 13μl ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 μg of dsRNA was transfected into 1x106 D-Mel cells using Transfast transfection reagent (#E2431, Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice received an intraperitoneal injection of 100 µl D-luciferin (stock of 40 mg/mL, Promega, E160). Mice were allowed to move freely for five minutes post injection prior to initiating anesthesia via isoflurane ...
-
bioRxiv - Biochemistry 2024Quote: ... Mice were anaesthetized by vaporized isoflurane and injected subcutaneously (s.c.) with D-luciferin (2.5 mg/mouse Promega) in 100 μl PBS vehicle ...
-
bioRxiv - Cancer Biology 2024Quote: ... mice were anesthetized with isoflurane and injected intraperitoneally with D-luciferin potassium salt (Promega, Madison, WI, USA). Ten to fifteen min after injection ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by the addition of 5-μL Endo-H reaction buffer and 5-μL Endo-H (Promega Cat#PRV4875, 2,500-U). Deglycosylation proceeded for 4-hours at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... Transcription of tRNApheV and tRNAleuZ was performed with 20 U T7 RNA Polymerase (37 °C for 90 min) using the Riboprobe® in vitro Transcription System (Promega) essentially as described in the instructions manual ...
-
bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on Cytation 3 (BioTek ...
-
bioRxiv - Microbiology 2021Quote: ... labeling was promptly carried out in an RNase-free environment in the presence of RNasin at 0.133 U/ml (Promega, Southampton; [49]).
-
bioRxiv - Neuroscience 2020Quote: ... total RNA was placed in 4 µl of lysis buffer (0.1% vol/vol Triton X-100, 2.5 mM dNTPs, 2.5 µM oligo-dT, 1 U/µl Promega RNasin Plus RNase inhibitor). Reverse transcription was performed followed by cDNA amplification ...
-
bioRxiv - Biochemistry 2020Quote: ... The RNA was added to the 100 µl SA Sepharose slurry together with 1 µl RNasin Plus RNase inhibitor (40 U/µL, Promega, N261A). 10 µl of the supernatant was saved for extraction of input RNA using TRIzol (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... cell pellets were lysed in polysome lysis buffer (gradient buffer containing 100 mM KCl, 10 mM MgCl2, 0.1% NP-40, 2 mM DTT, and 40 U/ml RNasin; Promega, Leiden, Netherlands), and onto 17–50% sucrose gradients and ultracentrifuged for 2 h at 40,000 rpm ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were treated 8 h post-transfection with or without IFN-α (1000 U/ml) before lysis 16 h later using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
-
bioRxiv - Immunology 2023Quote: A total of 125 ng genomic RVFV clone 13 RNA was either mock treated or incubated with 40 U CIAP (Promega, M1821), 2 U RNaseIII (Ambion ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 ml ice-cold wash buffer 1 (1x PBS with 2 % BSA, 1 mM DTT and 0.5 U/µl RNasin® Plus Ribonuclease Inhibitor - Promega #N2615) was added and cells were spun at 500 g for 3 min at 4 C ...
-
bioRxiv - Neuroscience 2024Quote: ... Supernatants were discarded and nuclei pellets were washed once with 4 mL Nuclei EZ Lysis Buffer and once with 4 mL of Nuclei Suspension Buffer (NSB) (PBS w/o Mg and Ca; 0.01% BSA; Biolabs ref B9000S; 40 U/mL RNAsin; Promega ref N251B). Final pellets were resuspended in 0.5 mL NSB containing DAPI (1 µL at 1 mg/mL ...
-
bioRxiv - Neuroscience 2024Quote: ... cell pellets were washed with 1× PBS and resuspended in either RNA lysis buffer (mirVana kit) containing 0.5 U/μL RNAsin Plus (Promega; Madison, WI) for RNA isolation or 1X radioimmunoprecipitation assay (RIPA ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were washed in 1 x PBS followed by lysis in 300 μl of cell lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% NP-40, 1×EDTA-free Proteinase Inhibitor, and 1000 U RNase I, Promega, Madison, WI, USA). The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclei were washed with Wash Buffer three more times and nuclei pellets were resuspended in Diluted Nuclei Buffer (1x Nuclei Buffer, 10x genomicsTM, supplemented with 1 mM DTT and 1 U/uL RnaseIn, Promega©) for counting and scMulti library preparation ...
-
bioRxiv - Cell Biology 2024Quote: ... Tumor growth was monitored using bioluminescence imaging by injecting D-luciferin (150 mg/kg i.p.; Promega, Madison, WI) and subsequently imaging mice on an IVIS Spectrum Imaging System (PerkinElmer ...
-
bioRxiv - Cancer Biology 2024Quote: ... Animals were injected with an intraperitoneal dose of 100 mg/kg D-luciferin (Promega, Charbonnières-les-Bains, France) and after five minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor growth was monitored using bioluminescence imaging by injecting D-luciferin (150 mg/kg i.p.; Promega, Madison, WI) and subsequently imaging mice on an IVIS Spectrum Imaging System (PerkinElmer ...
-
bioRxiv - Genetics 2021Quote: ... and 50 million nuclei per liver were incubated in a volume of 950 ul with 32 U of RQ1 RNase-free DNase I (Promega, cat. # M6101) for 2 min at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
-
bioRxiv - Microbiology 2022Quote: ... The PCRs were performed in a final volume of 20 μl of amplification mix containing 1 U of GoTaq polymerase (Promega, Madison, WI), 1× GoTaq buffer ...
-
bioRxiv - Microbiology 2022Quote: ... The resulting materials were used as template to generate cDNA using 100 U Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI), 0.45 μM N6 random hexamer primers (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were treated with IFNα (1000 U/mL) for 12 h and were harvested to conduct dual-luciferase reporter assay (Promega, Madison, WI). Firefly luciferase activity values were normalized to Renilla luciferase activity ...
-
bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... In a pre-warmed solution of Reconstitution Buffer (20mM HEPES, 250mM KCl, 2mM TCEP, pH 7.5) containing 40 U/mL RNAsin (Cat#N2111, Promega, Madison, WI, USA), each Sm protein sub-complex was combined to a final concentration of 8 µM and incubated for 5 min at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analyzed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, 1× EDTA-free Proteinase Inhibitor, and 1000 U RNase I Promega, Madison, WI, USA). Supernatant obtained after sonication and centrifugation steps were digested with DNase I for 20 min at 37°C ...
-
bioRxiv - Synthetic Biology 2024Quote: ... a mastermix was first prepared containing transcription reagents: T7 polymerase (27 U per 100 μL reaction; Promega, Madison, WI, cat. no. P2077), 1 mM DTT and 0.5 mM rNTPs (Promega ...