Labshake search
Citations for Promega :
501 - 550 of 653 citations for D Galactose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Chromatin-bound proteins were solubilised by incubation with 1320 U of DNase I (RQ1 RNase-free DNase I, Promega) on ice for 20 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA-dependent interactions were assessed by treating nuclear extracts with 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) as control or 1 mg/ml of RNase A (Sigma ...
-
bioRxiv - Zoology 2020Quote: ... 0.2 μl of Acetylated BSA at 10 μg/μl and 0.5 μl of 10 U/μl restriction enzyme Alu I (Promega), 12.3 μl of DNase-free water and 5 μl of PCR products ...
-
bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were lysed by adding 3x lysis buffer (10 mM Tris-HCl, 1.5% Igepal, 150 mM NaCl, 1 U/µl RNasin (Promega)) ...
-
bioRxiv - Biochemistry 2020Quote: ... Reactions containing 2.5 pmol protein and 3 pmol 32P-labelled RNA were carried out in a volume of 10 µL with 0.5 U/μl RNasin (Promega), 100 mM HEPES(NaOH ...
-
bioRxiv - Genomics 2021Quote: ... ligation was performed at 15°C for 16 hours using 100 ng of the digested DNAs and 4.5 U of T4 DNA ligase (Promega) in a final volume of 100 μl ...
-
bioRxiv - Biochemistry 2023Quote: ... penicillin 100 U/ml and streptomycin 100 μg/ml) +/-HaloTag® NanoBRET™ 618 Ligand (0.5 μl/ml, Promega). 4 h later ...
-
bioRxiv - Neuroscience 2023Quote: ... after which the slice pieces were fixed in 4% PFA in PBS (with 0.1 U/ml RNase inhibitor, Promega) for 15 mins and dissociated into single cells through careful trituration ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
-
bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
-
bioRxiv - Biochemistry 2022Quote: ... and penicillin (100 U/ml)/ streptomycin (100 μg/ml) (complete media) then transfected with Fugene 6 (Promega, Madison, WI). Cells were then lysed 2 days post-transfection using a lysis buffer containing 150 mM NaCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in presence of 1 mM Adenylyl-imidodiphosphate (AMP-PNP) and RNase inhibitor RNAsin (0.5 U, Promega) for 25 min at 4°C with head-over-end rotation ...
-
bioRxiv - Molecular Biology 2023Quote: ... Thirty-five cycles were done in a 50 µL reaction volume with 0.3 U GoTaqG2 Flexi (Promega, Madison, WI), 2.5 mM MgCl2 ...
-
bioRxiv - Genomics 2024Quote: In total 120k nuclei were sorted using an SH800 sorter (Sony) into 87.5μl of collection buffer (1 U/µl RNAsin (Promega, N2515), 5% fatty acid-free BSA (Proliant ...
-
bioRxiv - Genetics 2024Quote: ... Each cDNA sample was amplified in quadruplicate in a 25-μL mixture containing 0.75 U GoTaq DNA polymerase (Promega), 200 μM dNTPs ...
-
bioRxiv - Physiology 2024Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... and was reverse transcribed into cDNAs using oligo-d(T) and M-MLV reverse transcriptase (Promega). Real-time PCR was performed with the specific primers listed in Table 2 using the LightCycler 480 (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... Then 50 µL of lysates were mixed to 50 µl D-luciferine (Luciferase assay system, Promega) and the Firefly luciferase activity was determined for each cell lysate with an Infinite 200 Pro (Tecan ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice received intraperitoneal injection of 50 μl 30 mg/ml d-luciferin (in PBS) (E1603, Promega). Continuous administration of isofluorane gas was provided to maintain anaesthesia of animals during imaging ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fifty microliters of D-luciferin (30 mg/mL in saline) was injected subcutaneously in mice (Promega). Twenty minutes after the injection ...
-
bioRxiv - Immunology 2024Quote: ... TNF-α (human; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). For THP-1 viability assays ...
-
bioRxiv - Molecular Biology 2024Quote: ... mice received an intraperitoneal injection of 150 µg g-1 of D-luciferin (Promega, Charbonnières France), and were then anesthetized (isoflurane 4 % for induction and 1.5 % for maintenance ...
-
bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 10 μL of isolated RNA with 1 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
-
bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
-
bioRxiv - Genetics 2022Quote: Chromatin accessibility by real-time PCR (ChART-PCR) was performed as previously described (75) using 20 U DNase I (Promega). To assess nucleosome occupancy ...
-
bioRxiv - Genomics 2020Quote: ... Supernatant was removed and pellet was resuspended in 400 μl of sort buffer (1 mM EDTA 0.2 U/μl RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 pmols of folded pre-tRNA substrate was incubated with a final concentration of 5 U ml−1 RNasin plus inhibitor (Promega), 5 mM ATP and 8 pmols of TSEN complex in a final reaction volume of 20 μl for 1 h at 30 °C ...
-
bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 ug (for cellular RNA) and 20 ng (for EV RNA) was treated with 1 U of DNAse I RNAse-free (#EN0521 PROMEGA) according to manufacturer instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The PCR reaction was carried out in a 50 μl reaction volume containing 1.25 U GoTaq Flexi DNA Polymerase (Promega, USA), 10 μl of 5X Flexi Reaction Buffer (Promega ...
-
bioRxiv - Cancer Biology 2021Quote: The 3’UTR of EIF3A (or mutants lacking U/TAG motif) were cloned downstream of the Renilla luciferase in the XhoI and NotI sites of psiCHECK2 vector (Promega, Madison ...
-
bioRxiv - Molecular Biology 2020Quote: ... negative control and input samples for 30 min at 37°C with shaking at 800 rpm with 0.05 U/µl of RQ1 RNase-Free DNase in 1X RQ1 RNase-Free DNase Reaction Buffer (Promega, # M6101). RNA was eluted from HisPur Cobalt Resin by incubating samples in a final volume of 100 µl of PBS with 0.4 mg/ml of Proteinase K (Macherey-Nagel ...
-
bioRxiv - Molecular Biology 2020Quote: ... Nuclei pellets were re-suspended in 100 µl of PBS containing 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) with or without 5 µg/µl of yeast tRNA (Thermo ...
-
bioRxiv - Plant Biology 2021Quote: ... Residual genomic DNA was then removed from each sample with 10 U of RNAse-free DNaseI (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... was added to a final concentration of 3 mM followed by the addition of 5 µl of T7 Enzyme Mix and 50 U RNasin RNase Inhibitor (Promega). To produce transcripts for mock transfections the GTP concentration was increased to 7.5 mM and cap analogues were not added ...
-
bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
-
bioRxiv - Molecular Biology 2021Quote: The RT-PCR was performed using 250 ngs of total or polysomal RNA that were reverse transcribed using 50 U of Superscript II (Promega) in a total volume of 25 μL at 42 °C for 1 h ...
-
bioRxiv - Cell Biology 2021Quote: ... Beyotime) for 10 min at 37°C and the other was treated with 1 U/ul RNase inhibitor (N2518, Promega). For chromatin extraction ...
-
bioRxiv - Molecular Biology 2023Quote: ... penicillin (100 U/ml) and streptomycin (100 μg/ml) in the presence or absence of compounds and HaloTag NanoBRET 618 Ligand (Promega) for 4 h ...
-
bioRxiv - Developmental Biology 2023Quote: ... The synthesis of cDNA was achieved by incubation in a 20 µl reaction mixture containing 200 U of MMLV Reverse Transcriptase (Promega) and 500 ng of random primer (Roche ...
-
bioRxiv - Developmental Biology 2023Quote: ... Whole chicken eyes were enucleated at E4 or E5 in PBS containing 0.2 U / µl RNasin Plus RNase Inhibitor (Promega, N2615). Eyes from 3 embryos were pooled for each condition ...
-
bioRxiv - Developmental Biology 2023Quote: ... Nuclei were washed 3 times by resuspending in cold PBS containing 2% fraction V bovine serum albumin and 0.2 U / µl RNase inhibitor (Promega, N2615). For multiome assay ...
-
bioRxiv - Genomics 2023Quote: ... Supernatant was removed and pellet was resuspended in 400 µL of sort buffer (1 mM EDTA 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... Supernatant was removed and pellet was resuspended in 400 µL of sort buffer (1 mM EDTA 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... transfected cells were treated with human IFN-γ at (100 U/mL for 24 hr) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on SpectraMax iD3 (Molecular Devices ...
-
bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
-
bioRxiv - Systems Biology 2023Quote: ... The panel of ssDNA-labelled antibodies (250 ng/ml for each antibody) was mixed with 2 U/µl RNAsin Plus (Promega) and 0.1% Triton X-100 in PBS:PFBB (1:1 ...
-
bioRxiv - Neuroscience 2022Quote: ... The cell pellet was resuspended in ice cold PBS containing 0.04% bovine serum albumin (BSA) and 0.5 U/μl RNasin Plus RNase inhibitor (Promega, Madison, WI) to achieve a concentration of ∼750-1200 cells/μl ...
-
bioRxiv - Genetics 2022Quote: ... The amplification was performed in 20 μL of reaction (2XPCR FailSafeTM PreMix E from Biosearch Technologies, 1.25 U of GoTaq DNA Polymerase (Promega), < 1μg DNA template and 0.5 μM of each specific primers ...