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Citations for Promega :
551 - 600 of 706 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... An amount corresponding to 5 % of the pull-down volume was saved for Western blot analyses and 1 µg of trypsin gold (Promega) was then added to the beads for 16 h at room temperature in a thermomixer at 600 RPM ...
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bioRxiv - Cell Biology 2024Quote: 2-3 days before the recording the cultures were transferred into a recording medium containing 5% FBS and 0.1 mM luciferin (Promega E1601) (Feeney et al. ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were digested to peptides by adding 25 µL of 50 mM ammonium bicarbonate containing 5 µg of trypsin (Promega) and incubating at 37 °C for 18 hours ...
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bioRxiv - Biochemistry 2024Quote: ... elution buffer I (2 M Urea, 50 mM Tris pH 7.5, 1 mM DTT and 5 ng/µl Trypsin (Promega, #V5111)) were added and samples were incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Oligonucleotides encoding guide RNAs targeting M18BP1 (5’- TTGTACTGAAAAAATCATCA-3’) were cloned into pX459-v2 and co-transfected using FuGENE 6 (Promega) with pUC19 containing a 1528 base pair stretch containing the mutated sequence of the locus of interest and homology arms ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were then incubated for 72 h at 37°C and 5% CO2 and cell viability determined by CellTiter-Glo Luminescent Cell Viability Assay (Promega). In short ...
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bioRxiv - Developmental Biology 2024Quote: ... a minimal promoter (5′-AGACACTAGAGGGTATATAATGGAAGCTCGACTTCCAG-3′) and 8x Gli1-binding sites (110) were cloned into the pGL3-Basic vector (Promega), in which Firefly luciferase was replaced with NanoLuc luciferase amplified from the pNLF-N [CMV/Hygro] vector (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... then resuspended in 1 gel volume of 25 mM ABC containing 5 ng/µL of digestion grade trypsin (Promega, V5111) and incubated at 37°C for 16 hours for a full digestion or for 15 minutes for a partial digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... and the chromatin-associated fraction was sonicated on a Bioruptor (5 times 20 sec on/off intervals) prior to DNase treatment using RQ1 DNase (Promega) and RNA extraction using Trizol ...
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bioRxiv - Microbiology 2024Quote: ... Purified DENV2 EDIII was biotinylated by incubating 100 µg of DENV2 EDIII with 5 nmol of the HaloTag PEG biotin ligand (Promega) at room temperature (RT ...
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bioRxiv - Microbiology 2024Quote: ... The 25 μl reaction contained 5 μl of PCR Green Buffer with magnesium chloride (Promega, Cat N° M7845, Madison, USA), 1 μl of 5 mM dNTP ...
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bioRxiv - Microbiology 2024Quote: ... After three rounds of washing in PBST the following Horseradish peroxidase-conjugated secondary antibodies were diluted in 5% milk-PBST and used with at a 1:10000 dilution anti-rabbit (W4018, Promega), 1:5000 anti-mouse (W402B ...
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bioRxiv - Molecular Biology 2024Quote: ... cells in each well were resuspended in 30 μL of TLR7-Fc or other Fc-tagged proteins (final concentration 5 μg/mL) in 0.5% BSA in 1x DPBS containing 200U/mL RNasein (Promega, N2511), and incubated on ice for 45 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Exponentially growing and stationary phase cells yielding a total OD600 of 800 were resuspended in 5 ml 1x FastBreak cell lysis buffer (Promega) for 30 minutes on ice and then sonicated afterwards ...
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bioRxiv - Neuroscience 2024Quote: Ensembl predicted regulatory region including the POU domain binding site on Aspm promoter/enhance (sequence from 5’-GAAAAAGTGGGCAGTAACTCGC-3’ to 5’-CAACCTTTCCCTGAGGACGATC-3’) was synthesized by Twist Bioscience and cloned into pGL3-Basic Luciferase Reporter vector (Promega) using the Gibson assembly method ...
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bioRxiv - Cell Biology 2024Quote: ... Tris 50mM pH8.0 and samples were heated 5 min at 90°C before digestion in a mix of 500 ng of LysC (Promega) at 37°C for 2h ...
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bioRxiv - Cell Biology 2024Quote: ... cDNAs were amplified with 6 PCR cycles using short Solexa P3/5 primers to allow beads-based size selection with ProNex Beads Size-selection Chemistry (Promega). In order to avoid over-amplification ...
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bioRxiv - Microbiology 2024Quote: ... and cells were transfected with 400 ng of promoter luciferase construct or cotransfected with a 1:4 ratio of promoter luciferase to expression plasmid using Fugene 6 (5:1 transfection reagent to DNA, Promega). pcDNA3.1 empty vector was used as carrier DNA during transfection ...
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bioRxiv - Biochemistry 2024Quote: ... and wild-type or mutant βarr1 with a C-terminal HA tag with a 1:5 DNA:FuGENE®6 (Promega) ratio according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... samples were centrifuged at 200× g for 5 minutes and then analyzed with the Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... a separate set of duplicate cell suspensions were measured at 475 nm excitation and 535 nm emission wavelengths after addition of 5 μM coelenterazine-h (Promega). PBS or 10 μM quinpirole was added to cell suspensions and BRET emissions were immediately measured again ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The Renilla luciferase activity associated with WT or uORF-mutated 5’UTRs was measured according to the manual of the Dual-Luciferase Reporter Assay System (Promega) 32 h after transfection and was normalized to the activity of firefly luciferase.
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bioRxiv - Molecular Biology 2020Quote: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Cancer Biology 2020Quote: CellTiter 96 AQueous One Solution Cell Proliferation Assay MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) was used according to the manufacturer’s protocol (Promega, Madison, WI) to assess proliferation of cells cultured in 96-well plates.
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bioRxiv - Cancer Biology 2020Quote: ... 25uL of reconstituted CellTiter-Glo Luminescent Cell Viability reagent was added to each well and incubated for 5 minutes (Promega, G7571). Luminescence readings were taken using the BioTek Synergy H1 Hybrid Reader ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... at 3500 cells/well and cell proliferation over 3-5 day periods was determined by measuring luminescence with CellTiter-Glo® assay kit (G7570, Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... Individual reactions conducted with UGA-CPES consisted of a 10 µL mixture containing 5 µL of GoTaq Green Master Mix 2X (Promega Corporation), 0.5 µL of template DNA ...
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bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
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bioRxiv - Biophysics 2022Quote: ... Blots were incubated with secondary antibodies for 1h at room-temperature (1:10000 in 5% skim-milk powder, anti-rb-HRP-conjugate (Promgea, W401B)/anti-ms-HRP-conjugate (Promega, W402B)) ...
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bioRxiv - Biochemistry 2022Quote: The crRNA (5’-AAUUUCUACUGUUGUAGAUGUGAUAAGUGGAAUGCCAUGUGGG-3’) was synthesized and purified using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega). The following DNA templates were used for the in vitro transcription reaction ...
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bioRxiv - Genomics 2022Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2,000 in 5% BSA in TBST) for 1 hour at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 5 mL of overnight cultures using the Wizard Genomic DNA Purification Kit (Promega Corp., Madison, WI, USA) and DNA concentration was measured using a Qubit™ Fluorometer and Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Bright-Glo reagent was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega, USA), with titers given in Relative Light Units (RLU ...
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bioRxiv - Microbiology 2021Quote: ... for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega, E1501).
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bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Microbiology 2020Quote: ... Amplifications were performed in a 50 µl final volume using 5 µl of DNA template and 2.5 units of GoTaq polymerase (Promega, Madison, WI). Final concentrations of MgCl2 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... miR159 and U6 RNA (see Table S3) were 5’ end-labelled with [γ-32P]ATP using T4 polynucleotide kinase (PNK) (Promega).
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was again removed and replenished with 30 mL phenol-red free Opti-MEM supplemented with 5 mM CT-TAMRA (Promega, G8251), except for no CT-TAMRA control wells ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were vigorously shaken at 80 rpm for 5 min prior to adding 75 µl of CellTiter-Glo 3D Reagent (Promega #G968B) to each well ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted 5 folds with distilled water and used in a reaction mix composed of 1x GoTaq qRT-PCR master mix (Promega, USA) and 10 nM primers for RT-qPCR ...