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Citations for Promega :
501 - 550 of 2430 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and reverse transcribed (RT) using the Reverse Transcription System kit (Promega). The resulting cDNA samples were amplified by TaqMan quantitative PCR using commercially available primer/probe kits from Applied Biosystems for BDNF (Gene Expression Assay Mm00432069_m1 ...
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bioRxiv - Genetics 2020Quote: RNA was reverse-transcribed using the GoScript RT kit (Promega A5000) following the kit protocol ...
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bioRxiv - Genetics 2019Quote: ... Reverse-transcription (RT) was performed using the MMLV retro-transcriptase (Promega) from 500 ng of total RNA in a final volume of 60 µl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μl reverse transcription enzyme M-MLV-RT (Promega, USA). PCR was performed using each specific primer set in a total volume of 20 μl containing 10 pmol of each primer ...
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bioRxiv - Microbiology 2022Quote: ... treatment and cDNA was synthesized using M-MLV RT (Promega, USA). Gene specific primers used (Table 4) ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription (RT) was performed with M-MLV enzyme kit (Promega) and qPCR was conducted with the Syber GreenER qPCR Super Mix Universal (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... digested overnight at RT with 500 ng of modified trypsin (Promega) in 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and sgE RNA standards have been outlined previously.31 Both PCR products were serially diluted to produce standard curves in the range of 5 x 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences,30 and were used at 300 nM and 200 nM ...
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bioRxiv - Physiology 2023Quote: ... cDNA was synthesized using Oligo-dTs and M-MLV RT (Promega). qRT-PCR was performed with the StepOnePlus System (Applied Biosystems ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... qPCR was performed using GoTaq 1-Step RT-qPCR (Promega, A6020) reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) was utilized to perform cDNA synthesis and qPCR ...
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bioRxiv - Microbiology 2024Quote: ... The reaction consisted of 0.5 µL Luna RT (Promega Cat#E3005S), 2.5 µL of 2X reaction mix ...
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bioRxiv - Microbiology 2024Quote: ... Turbo-treated RNA was then reverse transcribed with AMV RT (Promega) primed with random 9-mer (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... mice were injected intraperitoneally with 150 mg/kg body weight of d-luciferin (Promega) and 10 minutes later the mice were imaged using a Xenogen IVIS Lumina system with Living Image version 3.0 software (Caliper Life Sciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... using primers JZ294/JZ295 (Table S5) and genomic lambda cl857 DNA (Promega) methylated with 5mC CG methyltransferase M ...
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bioRxiv - Cell Biology 2021Quote: ... and processed for reverse transcription using oligo(dT)15 primers (Promega #C110A) and SuperScript III reverse transcriptase (Thermo Fisher Scientific #18080-044) ...
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bioRxiv - Plant Biology 2021Quote: ... 0.2 µM of each primer and 0.5 unit of GoTaq polymerase (Promega) in a total volume of 25 µl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... random primers (C118A) and MTS (G3581) were obtained from Promega (Courtaboeuf, France). QuantiFast® SYBER® Green PCR Kit (204054 ...
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bioRxiv - Neuroscience 2021Quote: 250ng of Total RNA was reverse transcribed with Random Primers (Promega C1181) and MMLV Reverse Transcriptase (Sigma M1302 ...
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bioRxiv - Cell Biology 2021Quote: ... was reverse transcribed using primer OSV 381 with AMV Reverse Transcriptase (Promega). The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was prepared from RNA (400 ng) by using random primers (Promega), GoScript buffer (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized by using AMV reverse transcriptase with random primers (Promega). TaqMan gene expression assays (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total RNA with 0.5 μg of random primers (Promega) and distilled water up to 10 μl of final volume were incubated at 70°C for 5 minutes and then kept on ice for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and using a random primer mix (Promega). The reactions were assembled in triplicates using the qPCRBio SyGreen Mix Lo-Rox Kit (PCRBiosystems ...
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bioRxiv - Genomics 2020Quote: ... total RNA was annealed with 0.1mg/ml oligo(dT)15 primer (Promega) and cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was reverse-transcribed to cDNA by using oligo-dT primer (Promega) and M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... then cDNA generated using M-MLV reverse transcriptase and random primers (Promega). Taqman (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µM each primer and GoTaq polymerase reagent (Promega, Madison, WI, USA) were subjected to thermal cycling ...
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bioRxiv - Genomics 2023Quote: ... 200 nM primers and one unit of Taq polymerase (M829B, Promega, USA) was used for various fragment amplifications ...
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table 3.
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table S3.
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were prepared with GoTaq PCR Master Mix (Promega) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR (GoTaq, Promega) was used to amplify specific regions of the SOC ...
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bioRxiv - Cell Biology 2022Quote: ... PCR buffer (Promega) and MgCl2 (2.5 mM) ...
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bioRxiv - Microbiology 2023Quote: ... PCR cleanup (Promega), and quantification ...
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bioRxiv - Molecular Biology 2020Quote: ... Prior to the addition of 1 μl of MMLV-RT enzyme (Promega), the reaction was incubated at 65°C in order to denature double stranded RNA ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription (RT) was performed using the M-MLV Reverse Transcriptase (Promega) and quantitative PCR (qPCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The isolated RNA was reverse transcribed into cDNA with MLV RT (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega. SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2020Quote: ... The standard curve was generated using GoTaq 1-Step RT-qPCR (Promega).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega. SARS-CoV-2 (2019- nCoV ...
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bioRxiv - Cell Biology 2021Quote: ... One µg RNA was reverse transcribed using the MLV RT kit (Promega). The GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested for 3h at RT with 1μg LysC (Promega) and then digested overnight at 37°C with 2μg of Trypsin ...
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bioRxiv - Molecular Biology 2022Quote: ... The GoTaq® qPCR and RT-qPCR Systems kit (Promega, Beijing, China) was used to quantify the transcript abundance on the ABI 7500 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed into cDNA using the Promega RT Kit (Promega, A3500). RT-qPCR was performed using specific primer sequences for FOXG1 (F-CGTTCAGCTACAACGCGCTCA ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR was performed using GoTaq qPCR SYBR Green Master Mix (Promega). GAPDH was used as an endogenous control (primer details are listed in Supplementary Table 3).
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bioRxiv - Genomics 2023Quote: ... This was followed by addition of 1 μL M-MLV RT (Promega) to initiate reverse transcription in a 20 μL reaction of the M-MLV buffer ...
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bioRxiv - Pathology 2019Quote: ... 150 µl of luciferin (2.25 mg) (VivoGlo ™ Luciferin, In Vivo Grid, Catalog #: P1041, Promega) was injected intraperitoneally ...
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bioRxiv - Microbiology 2022Quote: ... PCR was conducted in Promega PCR master mix (Promega, Madison, WI). SYBR green-based qPCR and probe-based qPCR was conducted in SsoAdvanced universal SYBR green supermix (Bio-Rad Canada ...