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Citations for Promega :
451 - 500 of 2430 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the presence of other main circulating arboviruses was evaluated by RT-qPCR using GoTaq® 1-Step RT-qPCR System (Promega, Madison, WI, USA). Specific diagnosis primers were used for Zika [30] ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription (RT) was performed with AMV reverse transcriptase (Promega) in final volume 20 μl for 1h at 42°C on 1 μg of total RNA in the presence of two reverse primers (1 μl of 5 μM solution each ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Microbiology 2020Quote: ... and then cDNA was produced using M-MLV RT (Promega) and random hexamers ...
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bioRxiv - Neuroscience 2022Quote: ... using GoTaq® Probe 1-Step RT-qPCR System (Promega). The CDC SARS-CoV-2 nucleoprotein N2 primer set was used for amplification ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using GoTaq qPCR Master Mix (Promega) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 μL of reverse transcriptase (M-MLV RT, Promega) and incubated the samples for 1 hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... using the GoTaq 1-Step RT-qPCR System (Promega, USA) and primers targeting the gene coding for the endogenous control β-actin ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR was performed using GoTaq qPCR Master mix (Promega). Cycling conditions were as follows ...
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bioRxiv - Immunology 2020Quote: ... using the MMLV-RT reverse transcriptase enzyme (Promega, Madison WI) to obtain the complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM dNTP) and 1 μl of AMV RT (Promega) was added and the sample was incubated at 42 °C for 1 hour ...
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bioRxiv - Physiology 2020Quote: ... Reverse transcription (RT) was performed with AMV-reverse transcriptase (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Biophysics 2023Quote: ... Reverse transcription was performed with M-MLV RT (Promega, USA) according to the manufacturer protocol with the primer Pow-TBE-3’ ...
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bioRxiv - Plant Biology 2022Quote: ... DNA digestion was performed using the RQ1 RNase-free DNase set (Cat No.: M6101, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed using 150 µl of Luciferase cell culture lysis buffer (Promega) and luminescence was assessed using 96-wells white plate ...
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bioRxiv - Cancer Biology 2019Quote: ... mice received 150 mg/kg D-Luciferin (Beetle luciferin, E1605; Promega, Charbonnières, France) intraperitoneally ...
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bioRxiv - Microbiology 2021Quote: ... injection of 150 mg/kg D-luciferin (Beetle Luciferin Potassium Salt, Promega, USA). Images were analyzed using LivingImage v4.3.1 software (PerkinElmer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 150 nM of LactC2-eGFP and 0.07 U μL−1 RQ1 DNase (Promega). Liposomes were then transferred to the tube with deposited dried precursor films ...
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bioRxiv - Immunology 2023Quote: ... The supernatant without beads was digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µL 50 mM ammonium bicarbonate followed by incubation at 37°C for 5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant without beads was incubated with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µl 50 mM ammonium bicarbonate at 37°C for 5 h ...
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bioRxiv - Cancer Biology 2024Quote: ... following intraperitoneal injections of 150 mg/kg D-luciferin (Promega, Alexandria, NSW, Australia) and under anaesthesia with isoflurane ...
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bioRxiv - Genomics 2020Quote: Total RNA was reverse transcribed to cDNA using random primers (Promega) and M-MLV Reverse Transcriptase RNase H (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... and reverse transcribed with GoScript Reverse Transcriptase with random primers (Promega). Real-time quantitative PCR was done using FAM-MGB-labeled Col6a3 (Mm00711678_m1 ...
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bioRxiv - Plant Biology 2020Quote: ... using M-MLV reverse transcriptase and oligo(dT)15 primer (Promega). Two ng of cDNA was used as template in qPCRs ...
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bioRxiv - Bioengineering 2020Quote: ... with specific primers (Suppl. Table 1) from pNL1.1 (Promega, Fitchburg, WI) and inserted between the SalI and BglII sites using NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was transcribed into cDNA using an oligo dTn primer (Promega) per reaction ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was transcribed into cDNA using an oligo dTn primer (Promega) per reaction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... an anchor-oligo(dT) primer (Table S1) and random hexamers (Promega). The reverse anchor and forward nested degenerated PGM primers derived from the oyster Crassostrea gigas and human Pgm-1 sequences were then used to perform the upstream amplification of cDNA fragments (see Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of 100 ng/µL random hexamer primers (Promega: C1181) was added to each sample ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 nM dT-primer and 4 U RNase Inhibitor (Promega, N2611). Reverse transcription was performed at 42°C for 90 min after filling up to 10 μl with RT buffer mix for a final concentration of 1x superscript II buffer (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA (500 ng) was reverse transcribed using random primers (Promega) and AMV Reverse Transcriptase (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) primer and reverse transcriptase ImProm-II (Promega, https://www.promega.com). Three biological replicates for each type of sample were used for sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) 18 primers and the GoScript™ Reverse Transcriptase (Promega). qRT-PCR was performed using gene-specific primers (Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNasin Plus RNase inhibitor and random primers (all from Promega). SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... 10X Primer extension buffer and 2 U AMV Reverse Transcriptase (Promega) were added to the annealing mixture and incubated for 1 hour at 42 °C ...
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bioRxiv - Plant Biology 2024Quote: ... according to the instructions: stock oligo(dT)15 primer (C1101, Promega) concentration was 100 ng/μl ...
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bioRxiv - Microbiology 2020Quote: ... a PCR reaction using the PCR Master Mix (Promega) and the primers rplU_Fwd and rplU_Rev (Table 2 ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed using GoTaq PCR Master Mix (Promega) with primers mo37 and ag489 ...
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bioRxiv - Biophysics 2022Quote: ... Two PCR reactions using GoTaq G2 PCR mix (Promega) and a pUC19 template were set up ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using PCR cleanup kit (Promega). Sanger sequencing was performed at Psomagen ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR reactions were set up in triplicates with GoTaq® qPCR Master Mix (Promega, United States) and run in a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... and set at room temperature for 30 min prior to reading with a GloMax luminometer (Promega). Integration time was set at 1 second 54,55.
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Microbiology 2022Quote: ... the second set of plates was analyzed by adding CellTiter-Glo 2.0 Reagent (Promega, G9242/3) and measuring luminescence on a GloMax Discover System (Promega) ...
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bioRxiv - Genetics 2019Quote: ... and cDNA synthesis (M-MLV RT kit, Promega Corporation, cat. #M170A) were performed according to manufacturer’s specifications [21] ...
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription was performed with an RT cDNA Synthesis Kit (Promega). Real-time PCR was performed with SYBR green SuperMix using an iCycler real-time PCR detection system (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences19 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generatioplan of the 18S and E SgRNA standards have been outlined previously.20 Both PCR products were serially diluted to produce standard curves in the range of 5 × 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and E SgRNA standards have been outlined previously.20 Both PCR products were serially diluted to produce standard curves in the range of 5 × 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences19 ...