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Citations for Promega :
451 - 500 of 2159 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Eluted proteins were digested with sequencing-grade trypsin (Promega). Peptide mixtures were separated using Evosep One (Evosep Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) using 2 μg Plasmid DNA per 50 μL expression reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were detected with an anti-mouse secondary antibody conjugated to HRP (Promega) at 1:5000 dilution in 5% fat-free milk ...
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bioRxiv - Biochemistry 2022Quote: ... but the secondary antibodies were goat anti-mouse HRP conjugated antibody (Promega Cat #W4021) and goat anti-rabbit HRP conjugated antibody (Promega Cat # W4011) ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies were visualized by using anti-rabbit immunoglobulin G-alkaline phosphatase secondary antibodies (Promega) and the CDP-star detection system (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL of GoTaq® Probe qPCR Master Mix (Promega) and 0.5 μL of the appropriate TaqMan Assay (20X ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μl of RNasin Plus RNase Inhibitor (Promega, N2611) and incubated for 2 hours at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Trypsin (Promega; 5 ng/mL in 20 mM ammonium bicarbonate) was added to the gel pieces and proteolysis was allowed to proceed overnight at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... RNase inhibitor at 5% of the reaction volume (RNasin, Promega), and T4 DNA ligase (4,000 U per 50 µL reaction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 U/μl GoTaq Flexi DNA Polymerase (Promega, WI, USA), and 1 μl of DNA template ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 μL 2x buffer for T4 DNA Ligase (Promega) were mixed and incubated at 55°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.05 μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2 μl 5X colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of 5 ng/μl sequencing grade trypsin (Promega), 50mM AHC ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 with 0.3 µg/ul Acetylated BSA (Promega). All RNAs used were labelled with 5’ FAM (IDT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5 μL/well of CellTiter-Glo 2.0 reagent (Promega G9241) was added to the wells of the 384-well plate and allowed to equilibrate for 10 minutes before luminescence intensity was recorded on a Tecan SPARK plate reader (luminescence module ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT and 5 μg/μl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Genetics 2022Quote: ... 5-mins after the addition of furimazine NanoLuc substrate (Promega). A range of expression ratios of the NanoLuc and HaloTag containing constructs within the cells was achieved by varying the proportion of each plasmid during transfection ...
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bioRxiv - Plant Biology 2022Quote: ... and 5 μL of RNasin Plus RNase Inhibitor (Promega, N2611) and incubated for 2 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The Nano-Glo® 5× reagent (furimazine substrate + buffer; Promega) was prepared and added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were then treated with 5 units of DNase (Promega) and incubated for an additional 30 min at 37°C before being loaded on a 15% urea-polyacrylamide gel ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL 5 ng/μL sequencing grade trypsin (Promega; V5111) prepared in 50 mM ABC supplemented with 1 mM CaCl2 were added to the gel pieces and incubated 30 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... A solution containing 5 μg of sequencing grade trypsin (Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... a mix was prepared consisting of 5 % PEG-8000 (Promega), 100 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL GoTaq Colorless Master Mix (Promega Corporation, Madison, Wisconsin) including 200 μM of each dNTP and 1 unit Taq DNA polymerase ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μl of M-MLV Reverse Transcriptase Buffer (Promega #M531A), 10 mM NTPs mixture and 200 U of MMLV (Promega #M170A ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µl/well NanoBRET™ Nano-Glo® Substrate (Promega) and Extracellular NanoLuc® Inhibitor (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Green GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Colorless GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
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bioRxiv - Genetics 2020Quote: ... Secondary antibodies were from Promega (catalog nos ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate Antibody (Promega-W4021). Immuno-blots were developed using enhanced chemiluminescence (Clarity -Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate antibody (Promega -W4011), Anti-Mouse IgG (H+L) ...
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bioRxiv - Genetics 2019Quote: ... HRP-conjugated secondary antibodies (Promega) were diluted 1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... DENV2 structural proteins were cloned into expression vector from Promega in frame with a C-terminal Flag tag ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Proteins were digested using MS grade trypsin (Promega Trypsin Gold) in a ratio of 1:50 for 12 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were digested with 6 ng/µl trypsin (Promega, UK) overnight at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were digested using sepharose bead-immobilized porcine trypsin (Promega) at a ratio of 50 ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted protein was first treated with PNGase-F (Promega) to remove the N-linked glycosylation and then analyzed by mass spectrometry to determine the N-terminal sequence ...
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bioRxiv - Biochemistry 2019Quote: ... Tryptic digestion: the proteins were digested with trypsin (Promega, USA) using filter-aided sample preparation methods[25] ...
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bioRxiv - Immunology 2021Quote: ... Proteins in gels were digested with sequencing-grade trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega).
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bioRxiv - Microbiology 2021Quote: ... the protein lysates were digested with trypsin (Promega, Madison, WI) at 37°C overnight and the digested samples were desalted with 1cc C18 Sep-Pak solid phase extraction cartridges (Milford ...
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bioRxiv - Microbiology 2019Quote: ... Protein digestion was performed with 1.5 µg of Trypsin (Promega) overnight at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins were enzymatically digested using sequencing grade modified trypsin (Promega) overnight at 37ºC ...