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Citations for IBA Life Sciences :
101 - 150 of 150 citations for Monoisononyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Immunology 2021Quote: Purified E1E2 + AR4A-StrepII complexes (1.0 μg ml™1 in TBS with 0.1% DDM) were coated for 2 h at room temperature on 96-well Strep-Tactin XT coated microplates (IBA LifeSciences). Plates were washed twice with TBS/0.05% Tween20 before incubating with serially diluted mAbs and CD81-LEL-hFc (R&D systems ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was then induced with anhydrotetracycline (final concentration 200 ng/mL; IBA Life Sciences, Germany) and carried out for 16 h at 20 °C and 1050 rpm shaking ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble membrane fraction was diluted 2-fold with buffer B and applied to a column packed with Strep-Tactin beads (IBA Lifesciences) by gravity flow (6–10 s/drop ...
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bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was pelleted by ultracentrifugation before placing lysate over a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Insoluble materials were discarded and clear lysates were added to Strep-Tactin sepharose (400 μL 50% slurry; IBA Lifesciences #2-1201-010) loaded columns (Bio-Rad Laboratories #732-6008 ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Molecular Biology 2023Quote: ... we added an additional purification step and incubated the Flag-eluted samples with Strep-TactinXT 4Flow beads (IBA lifesciences, 2-5030-010) for 2 hours ...
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bioRxiv - Immunology 2019Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
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bioRxiv - Immunology 2023Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences #2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001 ...
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bioRxiv - Biochemistry 2023Quote: ... After equilibration of the Strep-tactin XT Superflow resin (IBA lifesciences, 10 mL per liter of cell supernatant) in 0.1 M Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Strep-Tactin-DY649 (IBA LifeSciences, IF 1:2000), mouse anti-CIMPR (2G11 ...
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bioRxiv - Biochemistry 2021Quote: ... with half immunoprecipitated for FLAG-ATF6 with 15 μL G1 anti-DYKDDDK resin and the other half affinity purified for nsp3.1-ST using 15 μL Strep-Tactin XT Superflow High Capacity resin (IBA Life Sciences, 2-4030-002) and rocked at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) containing 2.5 mM desthiobiotin (IBA Lifesciences). Eluted protein was concentrated and run on a Superose 6 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:250, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:150, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic DY-549 (IBA LifeSciences, FC 1:500), Strep-Tactin-DY649 (IBA LifeSciences ...
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bioRxiv - Plant Biology 2020Quote: The supernatant was filtered through a 0.45 µm filter and applied to a 5 ml Strep-Tactin Superflow HC column (IBA Lifesciences), equilibrated in Buffer W (100 mM Tris-HCL ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered through a 5.0 µm syringe filter followed by 0.45 µm syringe filters and loaded on to 3 mL gravity flow column packed with StrepTactin Sepharose resin (IBA Lifesciences). The column was washed with wash buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... TWIN-Strep tagged proteins were then purified by incubating whole cell lysates from the transiently-transfected cell lines (0.5 ml) with 50 μl of MagStrep “type3” XT beads (IBA Life Sciences) for two-four hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Biochemistry 2019Quote: The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble material was removed by centrifugation (12,500g, 15 min) and the supernatant was incubated with 300 mL Strep-Tactin® Superflow® resin (IBA lifesciences) while rotating slowly for 2h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences). Subsequently ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM DTT) and 50 μl 1x Buffer BXT (IBA Lifesciences) dispensed into a single 96-well KF microtiter plate ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA) and the kinase was eluted in 1x Biotin elution buffer BXT (IBA lifesciences). Plk1 was isolated from bacteria ...
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bioRxiv - Plant Biology 2023Quote: ... and supplemented with 75 μl·ml-1 Strep-Tactin Macroprep (50% slurry) (IBA Lifesciences GmbH, Göttingen, Germany). Samples were incubated in a rotary wheel at 4°C for one hour ...
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bioRxiv - Molecular Biology 2020Quote: ... The elute was then incubated for 1 h at 4 °C with Strep-Tactin beads (IBA Lifesciences) before application to a gravity column ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were floated in a solution of 1:50000 Strep-Tactin HRP conjugate (IBA Lifesciences, Germany) with 0.1% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl and 1 mM EDTA and eluted with 3X volume of BXT buffer (IBA Life Sciences). Samples were run on 4-20% TGX gels (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted fractions were combined and incubated for 1 h at 4°C with Strep-Tactin agarose beads (IBA Lifesciences). Beads were applied to a gravity column ...
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bioRxiv - Molecular Biology 2022Quote: ... The column was washed until baseline and the protein was eluted with PBS containing 1 mM DTT and 2.5 mM desthiobiotin (IBA Lifesciences). Fractions were pooled based on the chromatogram and SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... were mixed in PBS in total volume of 50 μL for 1 h at RT and subsequently precipitated with Strep-Tactin beads (Iba Lifesciences). The mixtures were washed twice with PBS ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... were formed as described previously (Sidorenkov et al., 1998) using scaffold 1 (Table 6) and the RNA sequence: AUCGAGAGG (IBA life sciences). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl MagStrep type 3 XT bead slurry, IBA life sciences). Following 1 h of binding at room temperature on a rotator ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ZnF mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ARID mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Immunology 2021Quote: SEC purified HIV-1 Env-Soc trimers were tested for antigenicity using ELISA involving Microplates pre-coated with Strep-Tactin (IBA Life Sciences GmbH). Microplates were coated with 1 µg/ml SEC-purified gp140-Soc trimers in a volume of 100 µl/well of coating buffer (25 mM Tris-HCl ...