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Citations for IBA Life Sciences :
1 - 50 of 150 citations for Monoisononyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... A 1 ml Strep-Tactin XT 4Flow high-capacity cartridge (IBA Lifesciences 2-5027-001) was washed with 14 column volumes of wash buffer (50 mM Hepes pH 7.6 ...
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bioRxiv - Immunology 2021Quote: ... before staining cells with DY-549-labeled strep-tactin (1:100 dilution, IBA lifesciences, Cat #2-1565-050) or Allphycocyanin-labeled streptavidin (1:200 dilution ...
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bioRxiv - Immunology 2021Quote: ... and further purified using a 2 mL StrepTactin sepharose column (IBA Lifesciences Cat# 2-1201-002) and a Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat#2-1002-001) ...
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bioRxiv - Biochemistry 2023Quote: ... or 2 mL of Strep-Tactin® Sepharose® resin (IBA Lifesciences GmbH, #2-1201-002) for strep-II tagged proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
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bioRxiv - Genetics 2023Quote: ... 1 ml StrepTactin (IBA Lifesciences, Göttingen, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was mixed with 2 mL Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed extensively with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was mixed with 2 ml Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed with wash buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... streptactin DY-488 (IBA Lifesciences; Cat. # 2-1562-050; 1:500) was added to secondary antibody solution ...
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bioRxiv - Microbiology 2022Quote: ... StrepMAB-Classic (1:500, mouse monoclonal, IBA lifesciences, 2-1507-001), anti-SARS-CoV-2 Spike (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were plated on 7H10 plates containing kanamycin and 100 ng/ml anhydrotetracycline (ATc, IBA Life sciences) for the induction of the S ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 1 ml Strep-Tactin resin (IBA Lifesciences) for 2 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... The Streptag pulldown was completed in the same manner using 100 μL of MagStrep "type3" XT bead suspension (IBA Lifesciences, 2-4090-002), except elution was achieved by boiling with sample buffer at 95 °C for 2 minutes.
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bioRxiv - Biochemistry 2024Quote: ... 5 min) and incubated on ice for 30 min with 100 nM scFv and StrepTactin-XT DY647 conjugate (IBA Lifesciences, 2-1568-050) at a 1:200 dilution in 1% BSA in PBS ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with StrepMAB-Classic monoclonal antibody conjugated to HRP (0.05 µg/mL, #2-1509-001, Iba Lifesciences) for 2 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated with 1 mL Streptactin sepharose beads (IBA Life Sciences) for 1 h ...
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bioRxiv - Microbiology 2020Quote: Purified StrepII-tagged sE2 monomer (1.0 µg/mL in TBS) was coated for 2 hour at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences). Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity flow columns (2 mL) were prepared with 400 μL 50% Strep-Tactin® XT Superflow High Capacity suspension (IBA Lifesciences). Protein purification was performed according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated for 1 h at room temperature in blocking solution with mouse StrepMAB antibody (IBA Lifesciences, 1:750, 2-1507-001) for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8 ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was loaded on a 1-mL Strep-Tactin column (IBA Lifesciences). Purification was performed as specified by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was performed with Strep-Tactin Sepharose slurry (1 mL/L) (IBA Lifesciences), using 1xTris buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... and eluted with Strep-Tactin elution buffer (100 mM Tris-Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 2.5 mM desthiobiotin) (IBA Lifesciences). Elution fractions were analyzed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... and eluted with Strep-Tactin elution buffer (100 mM Tris-Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 2.5 mM desthiobiotin) (IBA Lifesciences). Eluate was concentrated with Amicon Ultra centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Microbiology 2022Quote: ... incubated with anti-StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 μg/ml, IBA Lifesciences #2-1509-001) for 1 hour ...
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: StrepMAB-Immo (anti-Twin-Strep-tag®; IBA Lifesciences; 2-1517-001; 1:4000), FUS (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... 3C-digested proteins were loaded into StrepTactin XT 4Flow® 1 mL gravity flow columns (Iba Lifesciences) and collected in the flow-through for quantification ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cleared extract was passed through 1 mL Strep - Tactin XT Superflow (IBA Lifesciences GmbH, Göttingen, Germany) column pre-equilibrated with 250C buffer (25 mM HEPES - KOH pH 7.6 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
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bioRxiv - Biophysics 2021Quote: ... and 100 µM ApU (IBA Lifesciences GmbH) were added to the solution and incubated for additional 10 min at 30°C ...
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bioRxiv - Biophysics 2023Quote: ... and 100 μM ApU (IBA Lifesciences GmbH) were added to the solution and incubated for 10 min at 30°C ...
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bioRxiv - Biophysics 2023Quote: ... and 100 µM ApU (IBA Lifesciences GmbH) were added to the solution and incubated for additional 10 min at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... and 100 μM ApU (IBA Lifesciences GmbH) to the solution and incubated for 10 min at 30°C ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Strep-Tactin XT resin (1 mL) was prepared following the manufacturer’s instructions for gravity flow purification (Iba Life Sciences). After loading the cleared lysate ...
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bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was subjected to affinity chromatography using 1 mL buffer equilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences-GmbH) and flow through was collected ...
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bioRxiv - Microbiology 2021Quote: ... Samples stained with trimeric SARS-CoV-2 RBD were incubated with primary antibody strepmabHRP (2-1509-001, IBA lifesciences) with specificity towards the TwinStrep-tag at the C-terminus of the trimeric protein for 120 hours in PBSGT with 0.1% saponin ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs from HLA-A*0201 Donors were either stimulated with 1 µg/mL CMV pp65 antigen peptide NLVPMVATV (HLA-A*0201) (IBA Lifesciences) for 1 h or not ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: Expression of each mutant in the membrane samples was evaluated through western blotting using 10 ng mL-1 Strep-Tactin conjugated with horseradish peroxidase (IBA Lifesciences) and using SYPRO Ruby (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 mM desthiobiotin (IBA lifesciences, 2-1000-002). The eluted fractions containing highest concentration of the expressed protein were pooled and dialyzed against the storage buffer containing 10mM Tris/HCl ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solution was then centrifuged 3 times at 20,000 × g at 4 °C for 10 min periods and the supernatant was used for purification using fast protein liquid chromatography (FPLC) and 1 mL Strep-Tactin Superflow high capacity column (IBA Lifesciences, Germany). After equilibration with 5 column volume (CV ...
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bioRxiv - Microbiology 2022Quote: ... and reacted with 100 μL of Strep-Tactin Sepharose beads (IBA Lifesciences) for 4 h at 4°C ...