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Citations for IBA Life Sciences :
351 - 400 of 580 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The PEI supernatant was then loaded onto a Strep-Tactin XT 4F HC resin (IBA Life Sciences) at a flow rate of 1 mL/min (approximately 1 mL bed volume per 100 mg total protein ...
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bioRxiv - Biochemistry 2022Quote: ... Streptactin XT 4F High-Capacity resin was from IBA Life Sciences. [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Biochemistry 2022Quote: ... The PEI supernatant was then loaded onto a Strep-Tactin XT 4F HC resin (IBA Life Sciences) at a flow rate of 1 mL/min (approximately 1 mL bed volume per 100 mg total protein ...
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bioRxiv - Microbiology 2022Quote: ... The Strep-Tactin XT (IBA lifesciences) at 2 µg/mL in acetate pH 5 was injected for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... and then 1mg of lysate was incubated with prewashed MagStrep “type 3” XT magnetic beads (IBA LifeSciences) overnight in IP buffer (150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed with MagStrep type 3 XT beads (Iba Life Sciences, Gottingen, Germany) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
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bioRxiv - Physiology 2022Quote: ... the thawed medium was loaded onto Strep-Tactin® Superflow® high capacity cartridge (IBA Lifesciences GmbH) pre-equilibrated in the binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... or streptactin resin (IBA Life Sciences) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-COV-2 NTD proteins were purified with Sepharose Strep-Tactin beads (IBA Life Sciences) as previously described [75].
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bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... and 10% Buffer BXT (IBA Lifesciences) and gently rotated for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... StrepMAB-Classic (1:500, mouse monoclonal, IBA lifesciences, 2-1507-001), anti-SARS-CoV-2 Spike (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... or Strep-Tactin®XT 4Flow high capacity resin (IBA life sciences, 2-5030-002). Bound proteins were eluted with 0.2 mg/mL 1xFLAG (DYKDDDK) ...
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bioRxiv - Immunology 2022Quote: ... The digest was rotated with 200 μL of Strep-Tactin Superflow resin (IBA Lifesciences) at room temperature (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were either kept untreated or were incubated with CMV pp65 antigen peptide (IBA Lifesciences) for 1 h at 37°C and washed once with medium ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was purified on strep-tactin resin (IBA Lifesciences, Germany). After extensive wash ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed magnetic Streptactin beads (100 μl, MagStrep type 3 XT, IBA Life Sciences, Gottingen, Germany) were added and incubated overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) containing 2.5 mM desthiobiotin (IBA Lifesciences). Eluted protein was concentrated and run on a Superose 6 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Molecular Biology 2022Quote: ... The column was washed until baseline and the protein was eluted with PBS containing 1 mM DTT and 2.5 mM desthiobiotin (IBA Lifesciences). Fractions were pooled based on the chromatogram and SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was incubated with Strep-Tactin Sepharose beads (IBA Lifesciences) for 1 h and washed with CaMKII lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
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bioRxiv - Biochemistry 2022Quote: ... it was treated for 1.5 h with a StrepTactin horse radish peroxidase conjugate (IBA Lifesciences), diluted 1:75,000 in PBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... was transformed with a pASK-IBA63plus vector (IBA Lifesciences) harbouring a wild type PheDH strep tag fusion construct (Gielen et al.14 ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions were applied to Streptactin superflow resin (IBA LifeSciences) to remove homomeric LRRC8C channels ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates from cells transfected with Sb1 were directly loaded onto 100-300 µl of Streptactin superflow resin (IBA LifeSciences) to purify SBP-tagged sybodies ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Biochemistry 2022Quote: ... the fractions eluting from the HisTrap column containing myosin were loaded on a Strep-Tactin gravity flow column (IBA Lifesciences) equilibrated with 100 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... A CM5 sensor chip (Cytiva) was prepared by EDC/NHS coupling of Strep-Tactin-XT (IBA Lifesciences). Running buffer was 8mM TRIS ...
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bioRxiv - Cell Biology 2022Quote: ... Strep-Tactin resin (IBA Lifesciences) was used for the affinity purification and the complex was eluted with the wash buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was loaded on Strep-Tactin (IBA life sciences) or Ni-NTA (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs from HLA-A*0201 Donors were either stimulated with 1 µg/mL CMV pp65 antigen peptide NLVPMVATV (HLA-A*0201) (IBA Lifesciences) for 1 h or not ...
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bioRxiv - Developmental Biology 2022Quote: ... Elution fractions containing the Tmod proteins were then loaded onto Streptactin XT resin (IBA Life Sciences, Göttingen, Germany) and washed with 20 column volumes of strep buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... according to manufacturer’s protocol and eluted with 1X BXT (IBA Life Sciences). IgGs were purified by gravity column using Protein G resin (Cytiva ...
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bioRxiv - Immunology 2022Quote: ... GPC-I53-50As were purified by gravity column using StrepTactin 4Flow resin (IBA Life Sciences) according to manufacturer’s protocol and eluted with 1X BXT (IBA Life Sciences) ...
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bioRxiv - Plant Biology 2022Quote: ... The previously used Strep-Tactin Superflow hc cartridges were replaced by Strep-Tactin Superflow XT columns (IBA Lifesciences), which makes it possible to replace the Tris pH 7.5 buffer system with MES pH 6.5 ...
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bioRxiv - Microbiology 2022Quote: ... Magnetic Strep-Tactin®XT coated beads (IBA Lifesciences GmbH) were equilibrated by washing 40 μl of bead slurry three times in lyses buffer not containing phosphatase or protease inhibitor ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... incubated with anti-StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 μg/ml, IBA Lifesciences #2-1509-001) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Culture supernatants were harvested 5 days post-transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... Streptactcin XT Spin Columns were purchased from IBA Life Sciences.
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bioRxiv - Immunology 2022Quote: ... pH 8.0 and supplemented with a biotin blocking solution (IBA Lifesciences). RBDs were purified by affinity chromatography on a Strep-Trap HP 5 ml column followed by elution with 50 mM biotin and buffer exchange into PBS ...
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bioRxiv - Microbiology 2022Quote: ... 10µL of Strep-Tactin MagStrep’ type3’ XT beads (IBA Lifesciences) previously equilibrated with buffer 1 was added for 30min at RT ...
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bioRxiv - Microbiology 2022Quote: ... and reacted with 100 μL of Strep-Tactin Sepharose beads (IBA Lifesciences) for 4 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and spike was purified using Strep-Tactin XT beads (IBA Lifesciences), followed by size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2022Quote: Gene 8 was cloned into pASG-IBA105 (IBA Lifesciences, Götingen, Germany) using the direct cloning method described in the StarGate® direct transfer cloning protocol (IBA Lifesciences ...
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bioRxiv - Biochemistry 2022Quote: ... using the direct cloning method described in the StarGate® direct transfer cloning protocol (IBA Lifesciences) with restriction enzyme Esp3I ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:250, IBA Lifesciences) for the unstained samples ...