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Citations for IBA Life Sciences :
501 - 550 of 580 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... Strep-Tactin-AP conjugate (Iba Lifesciences) (dilution 1:3,000 in 3 w/v % BSA/TBS-T) ...
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bioRxiv - Immunology 2021Quote: Purified E1E2 + AR4A-StrepII complexes (1.0 μg ml™1 in TBS with 0.1% DDM) were coated for 2 h at room temperature on 96-well Strep-Tactin XT coated microplates (IBA LifeSciences). Plates were washed twice with TBS/0.05% Tween20 before incubating with serially diluted mAbs and CD81-LEL-hFc (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The bound proteins were removed by boiling in sample buffer and subjected to immunoblotting analysis using mouse monoclonal anti-6xHis (Yao-Hong Biotech. Inc., TW) and Strep-tagII (IBA Lifesciences, Germany) primary antibodies and the goat anti-mouse IgG-HRP-conjugated secondary antibody (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-StrepII-HRP (IBA Lifesciences) antibodies.
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bioRxiv - Microbiology 2020Quote: ... or Strep-Tactin (IBA Lifesciences, Goettingen, Germany) columns ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of Nth1-Strep was performed using Strep-Tactin II Superflow (IBA lifesciences). Clb2-Cdk1 was purified from yeast cells overexpressing Clb2-TAP as described previously (52,53).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were eluted with SDS-PAGE sample buffer and bound Nth1-Strep was detected by Western blotting using StrepMAB-Classic antibody (IBA Lifesciences) and HRP-conjugated anti-mouse IgG antibody (Labas ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was incubated with Strep-Tactin Sepharose (IBA Lifesciences) with gentle shaking for 2 hours at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG-solubilized lysates were added to 5 μg of TBS-prewashed MagStrep XT magnetic beads (IBA Lifesciences) for 1 h with mild shaking ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...
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bioRxiv - Biophysics 2020Quote: ... and loaded onto a column pre-packed with StrepTactin resin (IBA Lifesciences). The eluents were concentrated and incubated with 3C protease at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and lysates were incubated with 100 μl Streptactin-sepharose (IBA Lifesciences, Göttingen, Germany) for 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... 800 μL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4ºC ...
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bioRxiv - Plant Biology 2020Quote: The supernatant was filtered through a 0.45 µm filter and applied to a 5 ml Strep-Tactin Superflow HC column (IBA Lifesciences), equilibrated in Buffer W (100 mM Tris-HCL ...
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bioRxiv - Microbiology 2020Quote: We detected ComEC using an antiserum (IBA Lifesciences) against a C-terminal twin-strep tag fusion to ComEC in the Pmtl-(comK comS ...
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bioRxiv - Biophysics 2020Quote: ... 500 nM fluorescently labeled (IBA Lifesciences) or biotinylated oligonucleotides (Biomers ...
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bioRxiv - Biochemistry 2020Quote: ... pH 8.0 and loaded into a Streptactin Superflow cartridge (IBA Lifesciences 2-1238-001). Cartridge was washed with 100 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble membrane fraction was diluted 2-fold with buffer B and applied to a column packed with Strep-Tactin beads (IBA Lifesciences) by gravity flow (6–10 s/drop ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was loaded on a strep-tactin (IBA Lifesciences, Germany) column equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
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Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoproteinbioRxiv - Biophysics 2020Quote: ... The transparent supernatant was passed over a Strep-Tactin column (IBA Lifesciences) and bound protein was eluted with PBS buffer + 2.5 mM desthiobiotin ...
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bioRxiv - Cancer Biology 2020Quote: ... Insoluble materials were discarded and clear lysates were added to Strep-Tactin sepharose (400 μL 50% slurry; IBA Lifesciences #2-1201-010) loaded columns (Bio-Rad Laboratories #732-6008 ...
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bioRxiv - Plant Biology 2020Quote: ... which were incubated for 30 min at 4°C with 50 ul of ferrimagnetic StrepTactin beads (MagStrep Type3 XT beads, IBA LifeSciences) preincubated in 0.1M Tris pH8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The yadA gene was excised from pIBA2-YadA using XbaI and SalI and re-ligated into pASK-IBA2C (IBA Lifesciences) using the same restriction enzymes to create pIBA2C-YadA.
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bioRxiv - Microbiology 2020Quote: A Chloramphenicol resistance cassette derived from pASK IBA4C (IBA Lifesciences) was chromosomally introduced into the YenI locus of the Ye WAC strain to discriminate between the Ye wt and the Ye YadA0 or the T3SS deficient strain (T3S0) ...
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bioRxiv - Microbiology 2020Quote: Purified StrepII-tagged sE2 monomer (1.0 µg/mL in TBS) was coated for 2 hour at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences). Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing sE2 was harvested after six days and clarified using vacuum-filtration (0.2 µ m) and sE2 was purified using Strep-TactinXT columns (IBA Life Sciences) by gravity flow (∼0.5-1.0 ml supernatant/min) ...
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bioRxiv - Plant Biology 2020Quote: ... and the supernatant loaded onto a column containing Strep-Tactin XT resin (IBA Life Sciences) and protein was purified according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
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bioRxiv - Plant Biology 2020Quote: ... Pre-washed Strep-Tactin XT resin (IBA Life Sciences) was then added to the cleared lysate and gently rotated at 4°C for 1 hr ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Microbiology 2020Quote: ... or Strep-Tactin (IBA Lifesciences, Goettingen, Germany) columns according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and purified from supernatant with a Gravity Flow Strep-Tactin Superflow high capacity column (IBA Lifesciences). Purified CD81-LEL was polished by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2020Quote: ... or Strep-tactin Superflow (IBA Lifesciences), depending on the purification tag exploited in the affinity step (indicated in Table S1) ...
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bioRxiv - Biophysics 2020Quote: ... The solubilized sample was mixed with Strep-Tactin® resin (IBA Lifesciences) and washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was mixed with 2 mL Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed extensively with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... after which the complex was passed over Strep-Tactin XT resin (IBA Lifesciences—Goettingen, Germany). Bound complex was eluted using Buffer BXT containing 50 mM biotin (IBA Lifesciences) ...
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bioRxiv - Microbiology 2020Quote: ... Primary CD4+ T cells were harvested by positive selection using a FABian automated enrichment system and CD4 isolation kit (IBA Lifesciences). Isolated T cells were suspended in complete RPMI-1640 medium supplemented with 5 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates were incubated with Strep-Tactin beads (IBA Lifesciences) at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The clarified supernatant was purified in two steps: via a Strep-Tactin XT column (IBA Lifesciences) followed by Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... or Strep-Tactin Sepharose (IBA Lifesciences) as appropriate ...
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bioRxiv - Biochemistry 2020Quote: ... or Strep-Tactin Sepharose (IBA Lifesciences) with gentle shaking for 8 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The S6Pecto protein was expressed in FreeStyle293 cells and isolated on a StrepTrap HP column following the addition of BioLock Biotin Blocking Solution (IBA Lifesciences) to the culture supernatant.
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bioRxiv - Immunology 2020Quote: Viral gp33 peptide (gp33-41, KAVYNFATM) was purchased from IBA lifesciences. 1 μg /mL was used for re-stimulation of BM CD8+ T lymphocytes along with PMA ...
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bioRxiv - Biochemistry 2020Quote: ... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... and a PAGE-purified primer with an internal Cy5 for generating the 60/60 construct was purchased from IBA Life Sciences (Gottingen, Germany). All nucleosomes were assembled on the 601 nucleosome positioning sequence ([44]).
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bioRxiv - Immunology 2020Quote: ... Secreted postfusion gB trimers were purified using Strep-Tactin resin (IBA Life Sciences) before being run over a Superose 6 size-exclusion column (GE Healthcare ...