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Citations for IBA Life Sciences :
1 - 50 of 580 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... 1mM EDTA and 2 mM biotin) and eluted with 3X volume of BXT buffer (IBA Life Sciences). Samples were run on 12% TGX gels (Biorad ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl and 1 mM EDTA and eluted with 3X volume of BXT buffer (IBA Life Sciences). Samples were run on 4-20% TGX gels (Biorad ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was incubated with Strep-Tactin®XT 4Flow®resin (IBA Life Sciences) resin for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The cleared lysate was loaded onto a gravity-flow column with Strep-Tactin® Superflow® high capacity resin (IBA Lifesciences GmbH) pre-equilibrated with Buffer W ...
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bioRxiv - Plant Biology 2024Quote: ... and STA3 (soluble starch synthase III; Cre06.g282000.t2.1) were cloned into the Escherichia coli expression vector pASK-IBA7 (IBA Lifesciences GmbH; www.iba-lifesciences.com/). Expression from this vector equips the target protein with an N-terminal Strep-tactin affinity tag (Strep-tag II) ...
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bioRxiv - Plant Biology 2024Quote: ... Oligonucleotides were designed using the Primer D’Singer 1.1 software (IBA Lifesciences GmbH) to amplify the coding sequences including the putative chloroplast targeting peptides (Table 1) ...
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bioRxiv - Immunology 2024Quote: ... and Streptactin-APC (IBA Lifesciences). B cells were simultaneously stained with rGP38-Streptactin-PE and rGP38-Streptactin-APC tetramers for 1 hour on ice ...
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bioRxiv - Immunology 2024Quote: ... B cells were stained with rGP38 (IbAr10200) that had been tetramerized at 25 nM using Streptactin-PE (IBA Lifesciences) and Streptactin-APC (IBA Lifesciences) ...
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bioRxiv - Microbiology 2024Quote: ... Elution was done with 3X bead volume of BXT buffer (IBA Life Sciences) with 1% N-Dodecyl-β-D-maltoside ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was added to Strep-Tactin®XT 4Flow®resin (IBA Life Sciences) and incubated for 1 hour at 4°C with rocking ...
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bioRxiv - Microbiology 2024Quote: ... ReoM-Strep was purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the cell lysate was loaded onto preequilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences GmbH, Germany) and washed with wash buffer (500 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... then the soluble lysate was incubated with 1mL equilibrated Strep-Tactin® Sepharose® resin (IBA Lifesciences) rotating for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... then passed over Strep-Tactin Sepharose resin (IBA Lifesciences) by gravity ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified by Strep-Tactin Sepharose resin (IBA Lifesciences), washed with 5 column volumes of 1X PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and eluted with Strep-Tactin elution buffer (100 mM Tris-Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 2.5 mM desthiobiotin) (IBA Lifesciences). Elution fractions were analyzed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... and eluted with Strep-Tactin elution buffer (100 mM Tris-Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 2.5 mM desthiobiotin) (IBA Lifesciences). Eluate was concentrated with Amicon Ultra centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 mL polypropylene column was packed with 0.5 mL Strep-Tactin XT 4Flow resin (IBA Life Sciences), overlaid with 0.5 mL Strep wash buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... Cell debris were pelleted and clear lysate was loaded on Strep-Tactin® resin (IBA Lifesciences) pre-equilibrated with Strep Buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... GFP-RadA was eluted with Strep Buffer complemented with 2.5 mM desthiobiotin (IBA Lifesciences). The fusion protein was cleaved with ∼ 80 µg/mL TEV protease (home-made ...
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bioRxiv - Microbiology 2024Quote: The plasmid IBA13 (AmpR, IBA Lifesciences) was previously modified to harbour the gene encoding the superfolder Green Fluorescent Protein (sp-GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were floated in a solution of 1:50000 Strep-Tactin HRP conjugate (IBA Lifesciences, Germany) with 0.1% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: Purification was performed according to the manufacturer’s instructions (IBA Lifesciences, Germany). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... and Twin-Strep tagged proteins of interest were purified from crude extracts (soluble fraction) using the Strep-Tactin®XT Superflow® Kit (IBA Lifesciences, Germany). Purification was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Strep-tactin XT resin (IBA Lifesciences) was washed with 50 bed volumes of wash buffer ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ZnF mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... the ZnF was expressed as a fusion construct with an N-terminal Twin-Strep-tag® (IBA Lifesciences, Göttingen) and purified as the other constructs.
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bioRxiv - Evolutionary Biology 2024Quote: ... the proteins were eluted in 1x BXT buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 1mM EDTA; IBA LifeSciences). We then exchanged the buffer to the protein buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adding a Strep-tagII© (IBA Lifesciences GmbH) and thrombin cleavage site inserted in frame upstream of the coding sequence ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Strep-tagged proteins were captured on Strep-Tactin XT Superflow High Capacity (IBA LifeSciences) affinity resin ...
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bioRxiv - Microbiology 2024Quote: ... and passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifesciences) matrix in a gravity flow Omniprep column (BioRad) ...
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bioRxiv - Microbiology 2024Quote: ... Cell debris was removed by centrifugation at 30,000xg and 4°C for 45 min and the resulting crude extract was applied onto a Strep-Tactin®XT 4Flow® column (IBA Lifesciences, Germany) and purified following the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2024Quote: ... equilibrated in 25 mM KPi pH 7.8 and 500 mM NaCl) followed by Strep (Strep-Tactin Superflow high-capacity, IBA Lifesciences, equilibrated in 25 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5mM desthiobiotin (IBA Lifesciences)) ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Spike proteins in culture medium were purified with gravity flow columns using Strep-Tactin XT (IBA Life Sciences), concentrated to 0.5 mg/ml with ultrafiltration tubes (Millipore ...
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bioRxiv - Immunology 2024Quote: ... The concentrated medium was passed over Strep-Tactin Superflow Resin (IBA Life Sciences), washed with 1X PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The complex was purified by pulling down from the Strep-tag in AtRPAP3 with a StrepTactin®XT 4Flow® column (IBA Lifesciences) using the same protocol described for AtRPAP3 ...
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bioRxiv - Biochemistry 2024Quote: ... Pooled fractions containing AtRPAP3 were loaded onto a StrepTactin®XT 4Flow® high-capacity column (IBA Lifesciences) equilibrated in 25 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 min) and incubated on ice for 30 min with 100 nM scFv and StrepTactin-XT DY647 conjugate (IBA Lifesciences, 2-1568-050) at a 1:200 dilution in 1% BSA in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ARID mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... a Twin-Strep-tag (IBA Lifesciences, Göttingen) and ARID49-152 ...
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bioRxiv - Biochemistry 2024Quote: ... StrepTactin resin (IBA LifeSciences) was used to purify the S ectodomains ...
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bioRxiv - Biophysics 2024Quote: ... The synthetic gene sequence for nsp7-11C and nsp7-11N with suitable overhangs were cloned with Type IIS restriction enzymes into either pASK35+ and pASK33+ (IBA life sciences), generating a plasmid with C- and N-terminal His6-tag ...
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bioRxiv - Biochemistry 2024Quote: ... equilibrated in 25 mM KPi pH 7.8 and 500 mM NaCl) followed by Strep (Strep-Tactin Superflow high-capacity, IBA Lifesciences, equilibrated in 25 mM Tris pH 8.0 ...