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Citations for MP Biomedicals :
351 - 394 of 394 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... A 0.5 mL aliquot of the cell suspension was transferred to a FastPrep Lysing Matrix B (MP Biomedicals, Santa Ana, CA) tube containing 0.5 mL of PBS (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transduced with lentiviral particles at MOI of 10 in a 150 μL of cell culture media supplemented with 10 μg/mL of protamine sulfate (#02194729, MP Biomedicals) and saved as ReN-CAG-BirA ...
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bioRxiv - Systems Biology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using LSM Lymphocyte Separation Medium (MP Biomedicals, Santa Ana, CA). PBMCs were enriched for total monocytes using the Pan Monocyte Isolation Kit (Miltenyi Biotech ...
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bioRxiv - Microbiology 2022Quote: ... 600 μl of Trizol reagent was added and cells were then mechanically disrupted using a MP Biomedicals FastPrep Instrument and Lysing Matrix B tubes (MP Biomedicals). Black carbon particles were removed by centrifugation at 12 ...
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bioRxiv - Microbiology 2021Quote: ... pPol-I firefly Luc (NP gene; 100ng) and SV40 Renilla (50ng) reporter into HEK293T cells using polyethylenimine reagent (PEI, MP Biomedicals) at a ratio of 1:5 (DNA:PEI) ...
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bioRxiv - Microbiology 2021Quote: ... 1 x protease inhibitor (HALT cocktail, ThermoScientific) after the lysate was prepared following cell lysis via bead-beating (Fastprep Pro, MP Biomedicals). Immunoprecipitations were performed on 2-3 biological replicates.
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples of human healthy donors using Ficoll-Paque density centrifugation (MP biomedicals), frozen and then stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... resuspended at 5×108 bacteria /mL and lysed using a FastPrep-24 Tissue and Cell Homogenizer in Lysing Matrix B tubes (MP Biomedicals) with a setting of 4.0 m/s for 20s ...
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bioRxiv - Immunology 2022Quote: ... Transduction was carried out two times for 24 hours and transduced cells were selected with appropriate antibiotics (5 μg/ml blasticidin, MP Biomedicals, Santa Ana ...
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bioRxiv - Molecular Biology 2022Quote: ... were added to the pellet and bead-beaten at 6m/s for 30 seconds using an MP Fast Prep-24 Tissue and Cell Homogenizer (MP Biomedicals).
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bioRxiv - Microbiology 2022Quote: ... Harvested cells suspended in 100 μl of lysis buffer (74) were mechanically disrupted in FastPrep Lysis Matrix A tubes (MP Biomedicals) at 30 Hz for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and lysis was carried out by 6 x 1min maximum speed cycles on a FastPrep-24™ cell homogeniser (MP Biomedicals), with the samples being returned to ice for 5min between each cycle ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μM ZnSO4 and 2 mM PMSF and cells were lysed by glass bead lysis in the FastPrep-24 (MP Biomedicals). Pho8 activity was monitored by incubation of 200 μl lysate with 50 μl buffer 250 mM Tris–HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were disrupted by bead beating for 40 seconds at 6.5m/s in a FastPrep-24 instrument (MP Biomedicals, United states). The supernatant was transferred to an Eppendorf tube and chloroform (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... the larvae/embryos were mixed with 100 μL PBS in 1.5 mL tubes and homogenized with 3 cycles of 15 s at 6,500 rpm with rest intervals of 60 s by FastPrepTM 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and the supernatants were used as the virus suspension for RT-qPCR analysis or further investigation (passaging of viruses by being injected to new zebrafish embryos ...
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bioRxiv - Cell Biology 2023Quote: ... 200 µl of acid-washed beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Microbiology 2023Quote: ... One mL of the culture was centrifuged at 10,000 × g for 10 minutes and the cell pellet was used for DNA extraction using the FastDNA SPIN Kit (MP Biomedicals).
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bioRxiv - Cell Biology 2023Quote: ... 300 µl acid-washed glass beads were added and cells were mechanically lysed twice with a FastPrep device (6m/sec for 40 seconds; MP Biomedicals) with a 5-minute incubation on ice in between ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl acid-washed glass beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transfected or lentivirally transduced cells were selected and maintained in the same medium supplemented with 0.5 to 1 µg/mL puromycin (MP Biomedicals 194539) or 4 µg/mL blasticidin (US Biological Life Sciences B2104-30) ...
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bioRxiv - Microbiology 2023Quote: ... Only the initial cell lysis step was carried out in a FastPrep-24 instrument in lysing matrix A tubes (both MP Biomedicals) under different settings for the supernatant and cellular fraction samples ...
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bioRxiv - Immunology 2023Quote: ... DMEM (for labeling HeLa cells) or RPMI minus leucine (RPMI without L-leucine, L-glutamine, and sodium pyruvate from MP Biomedicals, supplemented with Glutamax and 1mM sodium pyruvate ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were distributed to two screw-cap microtubes containing 300 µl glass beads and lysed in a FastPrep instrument (MP Biomedicals) at 3 x 45 s 6 m/s ...
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bioRxiv - Immunology 2024Quote: To further label viral RNA, cells were washed twice in Hybridization Wash Buffer (HWB) (2X SSC, 10% formamide (MP Biomedicals FORMD002), 0.2 mg/mL Bovine Seric Albumin (BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Viral infections were carried out over 72 hours and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910).
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bioRxiv - Neuroscience 2021Quote: ... 50,000/well freshly dispersed anterior pituitary cells were plated on poly-L-lysine-coated 8-well multitest slides (MP Biomedicals, Aurora, OH), bathed in Earle’s medium 199 supplemented with 2.2 g/L sodium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... for SA14-14-2 endogenous pools of cysteine and methionine were depleted by treating cells for 2 h with Cys− Met− MEM (MP Biomedicals #1641454) containing 7.5 μg/ml Actinomycin-D (AMD ...
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bioRxiv - Genetics 2022Quote: ... lysis of cells and subsequent isolation of total RNA was carried out using the lysing matrix B/FastPrep® sample preparation system (MP Biomedicals) and the miRNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: The zebrafish larvae collected in Lysing Matrix Y tubes were homogenized with 3 cycles of 15 s at 6,500 RPM with rest intervals of 60 s by FastPrep™ 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... LNCaP and 22Rv1 cells were transduced at a multiplicity of infection (MOI) of 0.5 and selected with 1 µg/ml puromycin (MP Biomedicals Cat #194539) for 7 days ...
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bioRxiv - Cancer Biology 2022Quote: ... This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals, 196055). In total ...
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bioRxiv - Cell Biology 2024Quote: HEK293A or NG2-OSBP cells were seeded at approximately 50% confluence on 20 µg/ml entactin-collagen-laminin coated 8-well glass slides (MP biomedicals ICN6040805) and returned to the incubator ...
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bioRxiv - Microbiology 2021Quote: ... viral-induced CPE and cytotoxicity of the compound were determined by measuring the viable cells using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay (MP Biomedicals, NSW, Australia). Absorbance was measured at 540-650 nm on a plate reader.
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bioRxiv - Microbiology 2021Quote: ... Approximately 400 mg of 0.1 mm zircon/silica beads were added to the tube and subjected to mechanical cell lysis using a FastPrep-24 Classic bead beater (MP Biomedicals, Irvine, CA) at 4.0 m/s for 24 s ...
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bioRxiv - Immunology 2020Quote: ... the collected cells were vortexed for 30 seconds and were transferred to FastPrep® lysing matrix D tubes (MP Biomedicals; Auckland, New Zealand). To achieve cell lysis ...
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bioRxiv - Biochemistry 2022Quote: ... the cell lysates were scraped off the wells and collected into scintillation vials containing 7 mL EcoLite Liquid Scintillation Cocktail (MP Biomedicals: 01882475-CF). [14C] radioactivity in counts-per-minute (CPM ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were grown to semi-confluence in 35-mm Petri dishes containing HEK cell media [Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (MP Biomedicals, Solon, OH, United States), 2.0 mM L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... were added and the cells were lysed for 5 min at 2000 rpm in a FastPrep VR-96 instrument (MP Biomedicals, Santa Ana, CA). The beads were then precipitated by centrifugation for 5 min at 30 130 g and the supernatant was collected ...
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bioRxiv - Cancer Biology 2019Quote: ... Treated cells were fixed in formalin (10% buffered formalin phosphate, Fischer Scientific, Hampton NH) and permeabilized with Triton-X100 (MP Biomedicals, St. Ana CA). A primary conjugated antibody against CD44 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Ice cold 6% (v/v) PCA was added to the cells and the mixture was homogenized using a FASTPREP-24 (MP Biomedicals, Solon, Ohio, USA) and centrifuged at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Three 5 mm glass balls and 300 μL 10 mM MgCl2 were added to each tube and the plant cells were lysed at a speed of 4.0 m/s for 20 seconds in a FastPrep-24™ Instrument (MP Biomedicals, Illkirch-Graffenstaden, France) to release the live bacteria from the leaves ...
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bioRxiv - Immunology 2021Quote: ... Isolated PBMCs were then cryopreserved in a cryovial in cell recovery freezing medium containing 10% Dimethyl Sulfoxide (DMSO) (MP Biomedicals, LLC, Irvine, CA, USA) and 90% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... were added to the colon digesta slurry and cells were disrupted in 3 × 60 second cycles using a FastPrep24 (MP Biomedicals, Santa Ana, CA, USA). Debris were removed by centrifugation at 16.600 x g for 20 minutes and proteins were precipitated overnight in 16% ice-cold TCA ...