Labshake search
Citations for MP Biomedicals :
301 - 350 of 394 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... 0.6 g of glass beads were added and the cells were lysed in a FastPrep-24 (MP Biomedicals). Lysates were sonicated using a BioruptorPlus (Diagenode ...
-
bioRxiv - Neuroscience 2021Quote: ... 5-10 bacterial colonies per iPSC cell line tested were picked and grown in LB Medium (MP Biomedicals) for DNA extraction with Quick Plasmid MiniPrep Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2021Quote: Frozen cell pellets were suspended in PBS and lysed by mechanical agitation in a FastPrep 24 (MP Biomedicals) at 6.5 m/sec for 1 min ...
-
bioRxiv - Genomics 2019Quote: Fixed cells were washed in 1X PBS then resuspended in PBS containing 0.1% NP-40 (MP Biomedicals 0219859680), 0.5 mg/mL RNase A (Roche 10109169001 ...
-
bioRxiv - Microbiology 2022Quote: ... Cell lysis was accomplished by two steps of bead beating (FastPrep-24, MP Biomedicals, Santa Ana, CA, USA) for 30 s at 6.0 m s−1 ...
-
bioRxiv - Microbiology 2022Quote: ... and cells were lysed using the FastPrep®-24 Classic instrument with a COOLPREP™ adapter (MP Biomedicals). Bead beating was conducted at 6 ms−1 for 40 s for 2 cycles ...
-
bioRxiv - Immunology 2022Quote: Cells were isolated from peripheral blood by density gradient centrifugation over lymphocyte separation media (MP Biomedicals, #0850494-CF), washed in PBS and counted ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 500 µl 155 mM ammonium formiate using a FastPrep (MP biomedicals). Lipid were extracted corresponding to 400 µg protein by two-step extraction as described previously (Ejsing et al ...
-
bioRxiv - Cell Biology 2023Quote: ... Sheep Red blood cells (10% suspension) and rabbit anti-Sheep antibodies (IgG fraction) were purchased from MP biomedicals.
-
bioRxiv - Microbiology 2023Quote: ... Cells were disrupted by mechanical lysis in a FastPrep-24 instrument (MP Biomedicals, Santa Ana, CA, United States) in Lysing Matrix B tubes and RNA was purified with the Direct-zol RNA miniprep kit (Zymo Research ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in Lysis buffer and were disrupted with silica beads by bead-beating machine (MP Biomedicals™ FastPrep-24™ 5G Instrument ...
-
bioRxiv - Microbiology 2024Quote: ... resuspended cells were transferred to FastPrep Lysing Matrix B in a 2 ml tube (MP Biomedicals, no. 116911100) and lysis was achieved as described previously39 ...
-
bioRxiv - Molecular Biology 2020Quote: ... HCT116 ST cells were cultured in the presence of BrdU:BrdC (final concentration of 7.5 mM BrdU (MP Biomedicals 100166) and 2.5 mM BrdC (Sigma B5002) ...
-
bioRxiv - Microbiology 2021Quote: ... and the resuspended cells transferred to a FastPrep Lysing Matrix B in 2 ml tube (MP Biomedicals cat # 116911100). Samples were lysed using FastPrep bead beater for 40 seconds at 6 m/s ...
-
bioRxiv - Microbiology 2020Quote: ... virus was grown in MDCK cells at an MOI of 0.001 in DMEM supplemented with 0.3% bovine serum albumin (BSA, MP Biomedicals), 4.5 g/L D-glucose ...
-
bioRxiv - Microbiology 2022Quote: ... the pellets were resuspended in 3 mL DMEM/F-12 and the cell suspension was layered on a 1.040–1.089 g/mL discontinuous Percoll (MP Biomedicals) gradient ...
-
bioRxiv - Microbiology 2021Quote: ... and the resuspended cells transferred to a FastPrep Lysing Matrix B in 2 ml tube (MP Biomedicals cat # 116911100). Samples were lysed using FastPrep bead beater for 40 seconds at 6 m/s ...
-
bioRxiv - Biochemistry 2021Quote: ... The cell pellet was washed 1X time with PBS and then suspended in 1 ml RNAPro solution (MP Biomedicals). The cells were then added to a 2 mL tube containing 0.1 mm Lysing Matrix B beads (MP Biomedicals ...
-
bioRxiv - Biochemistry 2021Quote: ... The cells were then added to a 2 mL tube containing 0.1 mm Lysing Matrix B beads (MP Biomedicals) and were lysed using a Fastprep-24 bead beater(MP Biomedicals) ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were mechanically lysed three times for 30 sec with 0.1 mm silica beads (Lysing Matrix B, MP Biomedicals). The samples were then spun ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed by bead beating (3 cycles, 40s each, 6.0 m/s) using a FastPrep homogenizer (MP Biomedicals). Material was centrifuged at 13,000×g for 2 min to remove unlysed cells and insoluble debris ...
-
bioRxiv - Microbiology 2023Quote: ... The cell resuspension was lysed with 3 V of zirconia beads in a FastPrep-24TM 5G homogenizer (MP Biomedicals) for two cycles of 40 seconds at 6.5 m/sec with 1-minute rest in between ...
-
bioRxiv - Microbiology 2024Quote: ... and resuspended cells were disrupted using Lysing Matrix B tubes in a FastPrep-24 homogenizer (MP Biomedicals, Irvine, CA) at 6 M/s ...
-
bioRxiv - Microbiology 2019Quote: ... DNA was extracted from purified colonies using the phenol chloroform extraction method with the following modifications: glass beads (0.4mm) were used to lyse cells in a FastPrep-24 homogenizer (MP Biomedicals) with two cycles of 20 seconds at 4 m/s ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in TBSI and broken using 0.1 mm silica spheres (Lysing Matrix B) and FastPrep Homogenizer (MP Biomedicals) in 12 cycles of 30 s ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were lysed with 1 mL of acid-treated glass beads in a bead beater (FastPrep-5; MP Biomedicals) at level 7.5 for 15 s ...
-
bioRxiv - Molecular Biology 2019Quote: ... 200 μl of 0.5 mm zirconia/silica beads were added to samples and cells were lysed using the FastPrep-24 (MP Biomedicals) on max speed (6.5 m/s) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 200 μl of 0.5 mm zirconia/silica beads were added to samples and cells were lysed using the FastPrep-24 (MP Biomedicals) on max speed (6.5 m/s) ...
-
bioRxiv - Biochemistry 2019Quote: ... 10 to 25 mL cultures of exponentially growing cells were homogenized by glass bead-beating in a FastPrep (MP Biomedicals). Proteins extracted using either standard lysis buffer (WEB ...
-
bioRxiv - Microbiology 2020Quote: ... then adding 90 µL to each well such that the final concentration of the bile acid after the addition of cells (10 µL) ranged from 0.04 mM to 10 mM for CA (102897, MP Biomedicals), and 0.01 mM to 2.5 mM for DCA (D6750 ...
-
bioRxiv - Genetics 2020Quote: ... 0.3 g glass beads were added and the cells in the samples were lysed using a FastPrep-24 (MP Biomedicals). 100 µL of 3x SDS loading buffer was added ...
-
bioRxiv - Microbiology 2020Quote: ... using the Qiagen AllPrep DNA/RNA Mini Kit according to the manufacturer’s instructions with cell lysis by a bead beating step in Lysing Matrix E tubes (MP Biomedicals) in a FastPrep homogenizer (MP Biomedicals ...
-
bioRxiv - Immunology 2020Quote: ... and peripheral mononuclear cells (PBMCs) were isolated using standard density gradient centrifugation over lymphocyte separation media (MP Biomedicals, Solon, OH) and any contaminating red blood cells were lysed using hypotonic ammonium chloride solution ...
-
bioRxiv - Microbiology 2021Quote: ... Cell lines were routinely assessed for Mycoplasma contamination by PCR using a Myco-Sniff Mycoplasma PCR Detection Kit (MP Biomedicals). All parasite lines used and generated in this study are listed in Table S1.
-
bioRxiv - Genetics 2022Quote: ... Yeast cells were grown in YPD medium (2%) or in SC medium [containing yeast nitrogen base (1.7 g/liter; MP Biomedicals), ammonium sulfate (5 g/liter ...
-
bioRxiv - Immunology 2023Quote: ... Cells were subsequently washed with PBS and resuspended in serum- free RPMI with 3ug/mL Beta-2-microglobulin (MP Biomedicals) and the indicated concentration of peptide for 3 hours at room temperature followed by 3 hours at 37C in 5% CO2 ...
-
bioRxiv - Immunology 2022Quote: ... MDCK cells expressing IAV-NS1 (MDCK-NS1 cells) were infected with IAVdelNS1 in EMEM containing 0.35% bovine serum albumin (BSA, MP Biomedicals), 4 mM L-glutamine ...
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Fast DNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) according to the manufacturers’ protocol with a minor modification in which the gDNA was eluted in 1.0 mM Tris with 0.01 mM EDTA at pH 8 ...
-
bioRxiv - Biochemistry 2023Quote: ... Resuspended cells were placed in tubes containing 0.1-mm silica beads (Lysing matrix B tubes, catalog no. 6911-100; MP Biomedicals) and lysed in a bead beater (Bead Ruptor 24 Elite ...
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Fast DNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) as previously published (61) ...
-
bioRxiv - Microbiology 2024Quote: ... Total RNA are extracted after cell wall mechanical lysis with 0.1 µm beads (Precellys Evolution, Bertin Technologies) in RNApro reagent (MP Biomedicals), and purified by chloroform extraction and ethanol precipitation ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µL of cells were then spotted onto PBS agarose (1%) pads in 10-well multitest microscope slides (MP Biomedicals). Microscopy acquisition was performed using a Zeiss M1 upright microscope with a 100x oil-immersion objective ...
-
bioRxiv - Immunology 2024Quote: Primary peripheral blood mononuclear cells (PBMCs) were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). CD4+ T cells were negatively selected from PBMCs using CD4+ T cell isolation kit (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: ... Primary peripheral blood mononuclear cells (PBMCs) were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec) ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight from frozen glycerol stock in Luria-Bertani (LB) broth and 10 µg/mL tetracycline (MP Biomedicals).
-
bioRxiv - Microbiology 2023Quote: ... the cells were assessed for complement deposition by staining with goat anti-guinea pig C3-FITC (MP Biomedicals; catalog #0855385). After fixing ...
-
bioRxiv - Cell Biology 2020Quote: ... a 50-μL aliquot of cells from sporulation culture was pelleted and resuspended in 1 mg/mL Zymolyase-20T (#320921, MP Biomedicals) dissolved in water and incubated at 30°C for 10 minutes.
-
bioRxiv - Cell Biology 2020Quote: Yeast cells were grown either in rich medium (YPD) or in enriched complete synthetic medium (2x final concentration of CSM, MP Biomedicals) supplemented with 2% glucose or 2% galactose (wt/vol) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Membrane potentials (Vm) were monitored using the perforated whole-cell patch-clamp method with β-escin (MP Biomedicals, Santa Ana, CA) as the perforating agent (6 to 8 μM) ...
-
bioRxiv - Cell Biology 2022Quote: ... The following day the media on the cells were replaced with media containing a combination of manganese chloride (MP Biomedicals, 155334) and Actinonin (Sigma ...