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Citations for Sartorius :
201 - 250 of 984 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... followed by protein concentration using vivaspin centrifugal concentrators columns (MWCO 3.0 kD, Sartorius GmbH, Göttingen, Germany). Protein contents were analyzed according to Bradford using Rotiquant (Roth ...
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bioRxiv - Biochemistry 2023Quote: ... C-terminally biotinylated SKP1-FBXO22 fusion proteins (WT or C326A) were immobilized onto SA biosensors (Sartorius) to a response level of 1 nm ...
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bioRxiv - Microbiology 2024Quote: ... Protein-containing fractions were pooled and concentrated in a Vivaspin 20 centrifugal concentrator (50000 MWCO; Sartorius). Purified complexes were analyzed by spectrophotometry (Nanodrop ...
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were used to collect air on 37 mm sterile gelatin filters (3 µm pore size; Sartorius, Cat. No. 12602-37-ALK) containing a backing filter to ensure the gelatin filter remained intact during sampling ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were run on Superdex S200 size-exclusion column and concentrated with Vivaspin devices (Sartorius).
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Biochemistry 2022Quote: ... protein solutions were concentrated to about 12 mg ml-1 using a centricon centrifugal filter unit (Sartorius Stedim). The protein concentration was estimated by measuring the absorbance at 280 nm.
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and concentrated to 10 mg/ml using 10 kDa MWCO spin concentrators (Sartorius). Protein purity and identity were confirmed by detection of a single band by Coomassie-stained SDS-PAGE and by LC-MS/MS to confirm the expected sequence.
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Immunology 2023Quote: ... and 37.7H IgG were diluted to concentrations of 10 µg/mL and loaded onto Protein A sensors (Sartorius) to a binding signal of 1.0 nM ...
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bioRxiv - Bioengineering 2023Quote: ... was washed three times in DI water and concentrated to a final volume of 1 mL using 3 kDa MWCO ultrafiltration tubes (Sartorius Stedim Biotech, Germany). The tubes were spun at 4000 rpm for 40 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Protein samples were prepared using filter-aided sample preparation (FASP) digestion (Wisniewski et al., 2009) (Vicacon500, Sartorius, VN01H02 10kDA). The FASP filter were washed with 200 µl 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the protein-RNA complex were concentrated to 400-700 uM with Vivaspin 5-mL centrifugal devices with 10,000 MWCO (Sartorius). Before the measurements a 10% molar excess of RNA was added to saturate the protein as well as 10% v/v D2O ...
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bioRxiv - Microbiology 2023Quote: All eluted proteins were subsequently buffer exchanged to PBS or TBS by using Vivaspin20 ultrafiltration units (Sartorius, Gӧttingen, Germany) with MWCO 50 kDa (Env ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were spotted in duplicate onto a nitrocellulose 64 pad coated UniSart glass slide (Sartorius Stedim Biotech, Goettingen, Germany) or 24 pad coated AVID glass slide (Grace Bio-Labs ...
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Microbiology 2020Quote: ... The protein was loaded on a HiLoad 16/600 Superdex 200 pg column pre-equilibrated with storage buffer and the fractions containing pure Rel protein were collected and concentrated in a Vivaspin turbo 15 (Sartorius). The purity of protein preparations and the absence of RNA were assessed by SDS-PAGE and on a 1% agarose gel (in 1 × TAE buffer ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Immunology 2021Quote: ... Purified S proteins were exchanged to storage buffer and then filtered through 0.22 um filter (Sartorius Stedim Biotech, Göttingen, Germany). The concentration of purified protein was determined by ELISA ...
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bioRxiv - Immunology 2022Quote: KD measurements of the Pertuzumab and Trastuzumab IgMs to their Her2 antigen (Cat: 10004-HCCH, SinoBiological) were performed by first immobilizing them on Protein L (Cat: 18-5085, Sartorius) and CaptureSelect™ Biotin Anti-IgM Conjugate (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the antagonistic antibodies were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (PBS ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Biochemistry 2020Quote: ... bengalensis protein hydrolysates (BBPH) were subjected to centrifugal ultrafiltration using 3kDa cut-off membrane filtration unit (Vivaspin20, VS2091, Sartorius AG, Goettingen, Germany) to separate the low molecular peptide fraction (< 3kDa) ...
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Microbiology 2023Quote: ... Fc-tagged RBD proteins (20 μg/mL) were captured on the protein A biosensor followed by 30 s of baseline run in 1x kinetics buffer (Sartorius Cat#: 18-1105). The biosensors were blocked with a control Fc protein (Jackson ImmunoResearch Cat# ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). The recombinant proteins were further purified by ion exchange and gel filtration chromatography with ÄKTAprime plus system ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). For gel filtration ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Immunology 2024Quote: ... Supernatants were quantitated for antibody concentrations by bio-layer interferometry (BLI) on an Octet Red 96 (Sartorius) using protein A biosensors and a human IgG1 isotype control antibody as a standard ...