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Citations for Sartorius :
151 - 200 of 984 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated 10x using Vivaspin™ protein concentrator spin columns (Sartorius, VS15T02) and stored at 4°C.
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bioRxiv - Plant Biology 2021Quote: ... and loaded onto an ultrafiltration column (Vivaspin 3 or 10 kDa MWCO, PES, Sartorius, Palaiseau, France) for concentration and buffer exchange at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixed media were subsequently concentrated through spin columns with a cutoff of 3 kDa (Vivaspin, Sartorius). Mixed proteins were separated on a 4-20% Mini-PROTEAN TGX precast gel (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... they were concentrated using Vivaspin centrifugal concentrators with a cut-off of 3 kDa (Sartorius, VS2091) and stored at -20°C ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants containing the fibrils were recovered and concentrated on vivaspin® 3 KDa (Sartorius, VS04T91) at 3,000 g ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius) and washed each thrice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were concentrated using a Vivaspin6 10 kDa filter unit (Sartorius). The protein concentration was measured via absorption at 280 nm using a Nanodrop spectrophotometer (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 2-3 mg/ml using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... but visually identifiable amount (Supplementary Figure 3) by weighing powder on a microbalance (Sartorius BP 210 D). One hour before the release ...
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bioRxiv - Microbiology 2022Quote: ... Homogenates were centrifuged for 3 min at 17,000g and filtered using a 0.8 μm PES filter (Sartorius). Filtrates were treated with micrococcal nuclease (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... followed by centrifugation of denatured proteins on a filter device (Sartorius, Vivacon 500). After several washing steps with 8 M urea and ammonium bicarbonate ...
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bioRxiv - Biochemistry 2021Quote: ... The immobilization of ligand proteins on amine reactive second-generation (AR2G) biosensors (Sartorius) was done following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted to 100 nM in Kinetics buffer (Pall FortéBio/Sartorius). Buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... and aqueous solutions containing protein components were filtered using Minisart syringe filters (Sartorius). 0.5x1mm (I.DxO.D. ...
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bioRxiv - Synthetic Biology 2021Quote: ... The required amount of antibody was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
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bioRxiv - Microbiology 2020Quote: ... The homogenates were centrifuged for 3 min at 17,000 g and filtered with 0,8 μm PES filters (Sartorius). Filtrates were treated with benzonase (Novagen ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were filtered using Minisart 0.45 μm filter (Sartorius Stedim Biotech, Goettingen, Germany). Part of the extract was set aside prior to immunoprecipitation and used for SDS-PAGE as described above ...
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bioRxiv - Bioengineering 2023Quote: ... The AFC-eluates were buffer exchanged with 10kDa Vivaspin protein concentrator (Sartorius Stedim, Germany) using Sorval Lynx XTR centrifuge (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Proteins solutions were concentrated to at least 600 µM with Vivaspin centrifugal concentrators (Sartorius) and stored at -80 °C.
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bioRxiv - Plant Biology 2023Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Neuroscience 2024Quote: ... and Ulla proteins were concentrated to 35 ∼ 50 mg/ml using a VIVASPIN20 (Sartorius) concentrator and stored at −80 °C until use.
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bioRxiv - Microbiology 2019Quote: ... and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius). The protein was detected by SDS-PAGE (Supplementary Fig ...
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bioRxiv - Neuroscience 2020Quote: ... the iodixanol was removed from the AAV vector containing fraction by 3 rounds of diafiltration using Vivaspin 20 ultrafiltration devices (100’000 MWCO, PES membrane, Sartorius) and 1x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in the presence of a green mortality probe (1/3000, Incucyte® Caspase-3/7 Green Dye - Sartorius, Germany). Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Biochemistry 2022Quote: ... Protein complexes were concentrated to 25 µL in 30 kDa centrifugal concentrator (Vivaspin 500 Sartorius) and mixed with equal volume of 2 x native gel sample buffer (62.5 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius, Göttingen, Germany). Proteins were purified to homogeneity using a size-exclusion chromatography column (HiLoad® Superdex ...
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bioRxiv - Physiology 2023Quote: EV suspensions containing 50 µg protein were digested using FASP digestion (Vacacon500, Sartorius, VN01H02 10kDA), and sample volumes were adjusted to 100 µL with 8M urea ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We further weighted 3-week old plants with a precision balance (Sartorius AC 210 P with accuracy of 0.1 mg) to quantify variation in plant biomass ...
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bioRxiv - Biochemistry 2020Quote: ... were exchanged into phosphate buffer (pH 7.4) using disposable Vivaspin® 500 centrifugal concentrators with an MWCO of 3 kDa (Sartorius) to remove Cu2+ or H202 ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Biophysics 2021Quote: ... and 0.01 mg/mL 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were added before concentration in Vivaspin® 100,000 kDa molecular weight cut-off (MWCO) centrifugal concentrators (Sartorius) to a volume of ∼500 μL ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were additionally purified through a Superdex 200 26/60 size exclusion column (Pharmacia Biotech) and concentrated using a Vivaspin Turbo 3 kDa concentrator (Sartorius). Protein concentrations were measured using a UV/Vis spectrophotometer (GE Healthcare ...
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bioRxiv - Synthetic Biology 2022Quote: ... passivated (with 20 μL of 50 mM ABC in 1% sodium deoxycholate or SDC) Vivacon 500 3 kDa molecular weight cutoff (MWCO) filtration units from Sartorius Stedim Biotech ...
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bioRxiv - Synthetic Biology 2023Quote: ... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Plant Biology 2021Quote: ... and concentrated to the indicated total protein concentration (Vivaspin 20, 30 000 MW cut-off, Sartorius). For depletion assay ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g at 4 °C until a concentration factor of 20 was achieved ...
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bioRxiv - Immunology 2022Quote: ... Protein-containing fractions were pooled and rebuffered to PBS using VIVAspin columns (5,000 MWCO PES; Sartorius). Protein yield was determined with a BCA protein assay (Pierce™ ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were concentrated with Vivaspin 20-mL centrifugal devices with 5,000 or 10,000 MWCO (Sartorius) and buffer-exchanged into NMR buffer over PD-10 gel-filtration columns (GE-healthcare).