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Citations for Sartorius :
151 - 200 of 206 citations for Creatinine Serum Kit 4 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: 700 cancer and 1400 stellate cells were seeded in culture medium containing 1% FBS into the apical compartment of an Incucyte Clearview 96-well plate (Sartorius, 4582) at a 1:2 ratio ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... 50 µl media with 2 x compound concentration was added and plates were subsequently placed and monitored in an IncuCyte® (Sartorius) instrument ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 µl of single-cell suspension was dispensed on ready-to-use plates with an 8-channel electronic Picus® pipette (10 to 300 µl, #735361, Sartorius).
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bioRxiv - Developmental Biology 2024Quote: ... and 100,000 cells were resuspended in growth factor-free CNCC early maintenance media and seeded in an IncuCyte ImageLock 96-well plate (Sartorius 4379) in triplicate and incubated for 48 hours at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... was diluted to 50ug/mL in DPBS to coat the top and bottom sides of the inserts of an Incucyte Clearview 96-well plate (Sartorius: 4582). After 1h ...
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bioRxiv - Cell Biology 2023Quote: The XTT cell proliferation kit (BioInd, SARTORIUS) was performed according to the manufactural protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...
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bioRxiv - Microbiology 2019Quote: ... The eluted complex was then concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius). A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The resulting peptide solutions were centrifuged at 13,000 rpm for 15 min at 4 °C on tubes equipped with 2000 MW filtrate tubes (Sartorius, USA). Then ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was recorded for up to 7 days every 4 hours by the IncuCyte S3 Live-Cell Analysis System (Sartorius) with a phase-contrast microscope at 10X magnification ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing pure Fab16 were collected and concentrated to approximately 14 mg/ml using a VivaSpin 4 concentrator (10 kDa MWCO, Sartorius). Fab16 was crystallized by vapor diffusion at 4 °C by dispensing 200 nl of protein and 200 nl of crystallization buffer containing 1.5 M malic acid (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... cells and media were collected and this mixture was centrifuged at 3700xg at 4°C to remove debris and then concentrated by ultrafiltration using Vivaflow 200 (Sartorius). The purified AAV particles were collected after running the viral concentrate through an iodixanol column (Opti-Prep density gradient medium ...
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bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Cell Biology 2023Quote: MuSCs were freshly isolated and seeded on 0.2% gelatin-coated 96 well plates at a density of 2,000 cells per well in growing conditions and incubated for 4 days in an IncuCyte SX5 Live-Cell Analysis System (Sartorius) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein was eluted using similar buffer with 300 mM imidazole and concentrated by centrifugation with 4 kDa (AlgF) or 30 kDa (AlgJ and AlgX) cutoff Vivaspin Turbo centrifugal concentrators (Sartorius). His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare ...
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bioRxiv - Bioengineering 2024Quote: ... The plate was centrifuged for 5 minutes at 100g and then placed in the IncuCyte S3 Live-Cell Analysis System (Sartorius, G_ttingen, Germany) and stored at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Cancer Biology 2022Quote: ... Medium containing DMSO or increasing concentration of test compound was added to each well and spheroid growth was monitored for 4 days post-treatment using the IncuCyte® Spheroid Analysis system (Sartorius). Images from 4 days post-treatment and day 0 were analyzed using the IncuCyte® Spheroid Analysis software module ...
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bioRxiv - Microbiology 2021Quote: ... containing 50 μg/mL of propidium iodide (PI) and plates were then loaded in an IncuCyte S3 high-content imaging system (Essen Bioscience/Sartorius, Ann Arbor, MI). Longitudinal image acquisition and processing for virus-induced cytopathic effect (CPE ...
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bioRxiv - Cancer Biology 2022Quote: ... and 150 transparent (NTC-sgRNA) MCF7-dcas-9KRAB cells were seeded per well in a 96 wells ImageLock plate (Sartorius – cat no 4379) both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060) ...
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bioRxiv - Microbiology 2019Quote: ... viruses were concentrated by centrifugation at 4 °C using vivaspin 20 concentrators with a molecular weight cutoff of 100K (Sartorius, Göttingen, Germany). Thereafter ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was concentrated by centrifugation for 1 hour at 3220 g at 4°C in a 5kDa Cutoff Vivaspin tube (Sartorius, Cat # VS2012). ACM was aliquoted into low protein binding tubes (∼100ul per aliquot) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Afterwards flies were removed from the vial and allowed to air dry on the porcelain plates before they were weighed (Sartorius Quintix 124-1S microbalance) to the nearest 0.0001 grams and sexed ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was centrifuged at 100g for 1 minute and then placed in The IncuCyte® S3 Live-Cell Analysis System (Sartorius, Catalog No 4647), stored at 37°C ...
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bioRxiv - Systems Biology 2019Quote: ... and plates were immediately transferred to an IncuCyte Zoom dual color live content imaging system (Model 4459, Essen BioScience/Sartorius, Ann Arbor, MI, USA) housed within a Thermo tissue culture incubator (37°C ...
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bioRxiv - Plant Biology 2019Quote: ... These filtrates were ultra-filtered at 4°C through 20-ml Vivaspin micro-concentrators (10,000 molecular weight cut off, Sartorius Stedim, Biotech GmbH, Germany). Retained protein suspensions were used for protein content and enzyme assays ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was monitored via time-lapse image acquisition (2 images per well) every 4 hours for up to 96 hours using the IncuCyte(R) ZOOM software (Sartorius, vers. 2016B, 2018A). The initial acquisition was used as 0-hour timepoint ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Cancer Biology 2023Quote: Real-time calcein intensity was monitored using fluorescence live-cell imaging combined with a phase-contrast setup using the 4×objective (Sartorius AG, Gottingen, Germany). Images were acquired hourly and analyzed using Incucyte software and ImageJ.
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bioRxiv - Cancer Biology 2023Quote: ... The XTT-based cell proliferation kit (Sartorius, Beit Haemek, Israel) was used to quantitate viability according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The samples were weight with scales from KERN ALS 220-4 (KERN & SOHN GmbH, Balingen-Frommern, Germany) and Sartorius Cubis®-series (Sartorius AG, Göttingen, Germany). During the extraction process ...
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bioRxiv - Physiology 2023Quote: ... Bundles were lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for >4 h and weighed on a microbalance (Sartorius Cubis Microbalance, Gottingen, Germany). The rate of mH2O2 emission was calculated using a standard curve under the same assay conditions and then normalized to fibre bundle dry weight.
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bioRxiv - Cell Biology 2023Quote: ... of either parental or mNG-modified cells were plated per well in a 6-well plate in triplicate and placed in an Incucyte system (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Four images per well (total =12 per sample ...
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Neuroscience 2024Quote: ... then labeled with the pHrodo Red Cell Labeling Kit for Incucyte (4649; Sartorius, Bohemia, NY) with a final concentration of 125 ng/mL dye following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines tested negative for mycoplasma contamination using the Microsart AMP Mycoplasma Kit (Sartorius).
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bioRxiv - Physiology 2024Quote: ... Detection was done with the EZ-ECL Chemiluminescence Detection kit for HRP (20-500-120, Sartorius).
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...