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Citations for Sartorius :
401 - 450 of 484 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the proteins were identified by SDS-PAGE and concentrated using a Vivaspin Turbo 15 centrifuge filter with a 10 kDa molecular weight cutoff (Sartorius). Glycerol was added to a final concentration of 25% ...
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bioRxiv - Microbiology 2021Quote: ... 120- and 180-minutes post aerosolization by passing air at 6L/min for 30 seconds from the drum through a 47mm gelatin filter (Sartorius). Filters were dissolved in 10 mL of DMEM containing 10% FBS and stored at -80°C ...
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bioRxiv - Biochemistry 2020Quote: ... The buffer was exchanged into 100 μL of 50 mM ammonium acetate (Scientific Laboratory Supplies, U.K.) with 10K molecular-weight cut off filters (Vivaspin 500 polyethersulfone, 10kDa, Sartorius, Germany), and samples were then reduced with 4.2 μL of 5 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2021Quote: ... Purified S proteins were exchanged to storage buffer and then filtered through 0.22 um filter (Sartorius Stedim Biotech, Göttingen, Germany). The concentration of purified protein was determined by ELISA ...
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Pathology 2021Quote: ... the guts were washed with PBS to be placed and keep them stretched in 0.45 µm cellulose nitrate filters (Sartorius Biolab). Half of the bees in each cage and group were studied by light microscopy while the rest were processed for TEM ...
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bioRxiv - Microbiology 2020Quote: ... The virus containing medium was concentrated down to approximately 30 mL using a pump-powered filter (100-kDa cut-off; Vivaflow, Sartorius) and then dialysed into an excess of buffer (10 mM Tris pH 8.0-150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... buffer was exchanged to PBS (same volume of the initial serum) using 10 kDa MWCO Vivaspin6 filters (Sartorius, Gӧttingen, Germany) and the resulting preparations were used to measure BG505/T332N neutralization as described above.
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bioRxiv - Microbiology 2023Quote: ... Final separation was achieved following concentrating the Heparin elution to approximately 8 mg/mL using a 30 kDa MWCO Vivaspin filter (Sartorius), by applying the sample to a Superdex 200 10/300 GL (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Flow through of the 0.22 µm filters was subsequently concentrated using tangential flow filtration with a 100 kDa cassette (Sartorius Stedim). The concentrated samples were stored at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining vesicle pellets were resuspended in 2 mL sterile 1× PBS and sterile filtered with 0.20 µm syringe filters (Carl Roth, Sartorius VS0271).
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Neuroscience 2023Quote: ... Between 5 and 10 mL of plasma was diluted in PBS in a one-to-one ratio and filtered through a 0.45 μm pore size syringe filters (Stedim Minisart®, Sartorius). The plasma was then injected in HiTrap protein G HP (Cytiva) ...
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bioRxiv - Systems Biology 2023Quote: Extracellular concentrations of glucose and acetate were quantified in 180 µL of filtered broth (0.2 μm syringe filter, Sartorius, Germany) by 1D 1H-NMR on a Bruker Avance 500 MHz spectrometer equipped with a 5-mm z-gradient BBI probe (Bruker ...
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bioRxiv - Systems Biology 2023Quote: ... Cell dry weight was determined by vacuum filtration of 1 mL of samples on pre-weighed 0.45 µm filter membranes (Sartorius Biolab), followed by 15 min microwaving at 325 W and placement in a desiccator for at least 3 days.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and spiked solutions were degassed in an ultrasonic bath (Soner 210H, Rocker Scientific, Taiwan) for 15 min after being filtered through a 0.22 µm syringe filter (Sartorius, Germany). Dionex Chromeleon software was used to calculate data acquisition ...
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bioRxiv - Genomics 2024Quote: GFP was imaged using a BZ-X Filter FITC (Keyence OP-87764) on a BZ-X810 microscope (Keyence) or with a Incucyte S3 (Sartorius). Images for measurement were taken at 10× magnification ...
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bioRxiv - Biochemistry 2024Quote: Samples subjected to HMW fractionation were ultrafiltrated using 300 kDa cut-off filters (Vivaspin® 500 Polyethersulfone, 300 kDa (Sartorius)) pre-conditioned to PBS ...
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bioRxiv - Microbiology 2024Quote: ... and concentrated by centrifugation at 1500 x g at 4 °C using ultrafiltration devices with a filter cut-off at 100 kDa (Centrisart, Sartorius) [37] ...
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bioRxiv - Microbiology 2024Quote: ... A sample of 6 L of air per min was collected during the 10 min exposure on the 47 mm gelatin filter (Sartorius). Post-exposure ...
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 8000x g for 10 minutes and sterile filtered twice with 0.2 µm pore size Minisart Syringe Filter (Sartorius, Germany). This process was repeated after an additional incubation with the A ...
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bioRxiv - Microbiology 2024Quote: ... the tubes were centrifuged at 4600x g for 10 minutes and the supernatants were double sterile filtered with 0.2 µm pore size Minisart Syringe Filter (Sartorius, Germany), before storage in aliquots at −80 °C until use.
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bioRxiv - Microbiology 2024Quote: ... intracellular parasites were isolated by syringe passage with 23-gauge blunt needles (SAI Infusion Technologies) and filtration through a 5 μm membrane filter (Sartorius).
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bioRxiv - Neuroscience 2024Quote: ... at 50°C for a final concentration at 20mg/mL and filtered through sterile syringe filter (Minisart 0.2µm PES, S6534-FM6UK, Sartorius Stedim Biotech). Hsf1flox/flox:CAGCreERTM transgenic mice were intraperitoneally injected with the tamoxifen at 150 mg/kg/day for five consecutive days ...
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bioRxiv - Microbiology 2020Quote: ... and then a volume of 1.8 – 4.5 l of filtrate from the pre-filtration was filtered under mild vacuum (<100 mm Hg) through a 0.2 μm isopore polycarbonate filter (Sartorius, Göttingen, Germany) to collect microbial biomass ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Microbiology 2020Quote: ... then 100 mL of the appropriately diluted sample was filtered through a cellulose nitrate membrane filter with a pore size of 0.45 μm (Sartorius, Goettingen, Germany) using a vacuum pump ...
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bioRxiv - Immunology 2021Quote: Supernatants of Expi cultures and monoclonal hybridoma populations were collected, concentrated (Amicon, UFC810008) and filtered through a 0.2 μm filter (Sartorius, 16534-K). Affinities were then measured on an Octet RED96e machine (FortéBio ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Microbiology 2021Quote: ... using a centrifugation tube with an insert containing a molecular weight cut-off filter (5 kDa, Sartorius Stedim Biotech, Goettingen, Germany). Each HMW fraction was purified by flushing with 20 ml of water ...
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bioRxiv - Plant Biology 2021Quote: ... The amount of the peat moss biomass retained on the filter was weighed on a scale (E12000 S, Sartorius, Göttingen, Germany).
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Cell Biology 2021Quote: ... Urine samples considered for IFCM analyses were pre-cleared by filtration using 0.22 µm polyethersulfone syringe filters (Minisart, Sartorius, Göttingen, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... The supernatants were sterilized with the addition of gentamicin (Thermo) and passed through a 0.22-μm filter (Sartorius AG, Göttingen, Germany). The filtered supernatant was applied to a HisTrap HP nickel column (Cytiva ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... A total 75 ml culture supernatant was concentrated and exchanged with buffer W using 10 kD cutoff centrifugal filters (Sartorius, Germany). A 50 µl of Strep-Tactin®XT agarose beads were added to the concentrated sample in a final volume of 1 ml containing 1x protease inhibitor cocktail and incubated overnight at 4 ℃ ...
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Molecular Biology 2023Quote: ... The media was then filtered through a 0.45 µm filter (STARLAB, Hamburg, Germany) and concentrated using a 10 KDa Vivaflow tangential flow filtration (TFF) membrane (Sartorius, Göttingen, Germany) to a volume of 10-15 ml ...
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bioRxiv - Biochemistry 2024Quote: ... adding 3 ml of ice-cold Tris buffer and by filtrating the PLs through 0.45 μm pore-size membrane filters (Sartorius Stedim Biotech). Filters were washed twice with 3 ml of Tris buffer and allowed to dry ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Microbiology 2023Quote: ... A sample of 6 liters of air per min was collected during the <10 min exposure on the 47 mm gelatin filter (Sartorius, Germany). Post exposure ...
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bioRxiv - Plant Biology 2024Quote: ... Fractions were pooled and concentrated to a final concentration of 2 mg/ml using a centrifugal filter device (Sartorius, Göttingen, Germany) with a molecular weight cut-off of 50 kDa.
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bioRxiv - Microbiology 2023Quote: ... were used to collect air on 37 mm sterile gelatin filters (3 µm pore size; Sartorius, Cat. No. 12602-37-ALK) containing a backing filter to ensure the gelatin filter remained intact during sampling ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteolytic digestion was carried out according to the FASP protocol32 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) using 30 ug of protein resulting from each fraction ...