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Citations for Sartorius :
351 - 400 of 484 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... samples were clarified using a syringe with a filter cascade (5 µm, 1.2 µm, 0.8 µm, 0.45 µM, Sartorius AG). The cell pellets were not further analysed ...
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bioRxiv - Immunology 2024Quote: ... then concentrated by filtration though a Vivaflow 200 PES filter with a 10,000 molecular weight cut-off (Sartorius). Titers of MNoV stocks were determined by plaque assay on BV2 cells (75 ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed solution was finally concentrated by centrifugation through a Vivaspin Turbo 4 membrane filter (MWCO 10,000; Sartorius) at 3,500 × g for 30-45 min at 4°C.
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were obtained by centrifugation (8,000 g, 10 min) and filtration (0.22 μm filters; Sartorius, Göttingen, Germany). Stx production was tested using Vero cell cytotoxicity assay (supplement) ...
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bioRxiv - Molecular Biology 2022Quote: ... 80 rpm followed by overnight incubation at room temperature before filtering with a 0.2 μm syringe filter (Sartorius). To determine the phage titers ...
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bioRxiv - Microbiology 2022Quote: Aqueous samples were collected and filtered through a 0.45-μm syringe filter (Sartorius, Cat. No. 14-555-278) immediately after spiking in nitrogen and every 15 minutes for the subsequent hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted fractions were collected and concentrated with a VIVAspin 3,000-molecular weight cut-off filter column (Sartorius) and applied to a Superdex75 10/300 GL gel-filtration column (Cytiva ...
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bioRxiv - Bioengineering 2024Quote: ... The lysate was clarified by centrifugation at 10,000 g for 45 minutes and filtering through a 0.2 μm membrane filter (Cat # 17823, Sartorius). Mucinase was purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Bioengineering 2024Quote: ... Agent V treated conditioned media was clarified to remove cell debris by pumping through a depth filter (Sartorius). Pre-filter pressure was continuously monitored and maintained per the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant (cytosol fraction) was removed and concentrated with Vivaspin 500 Centrifugal Concentrator filters (cutoff, 3 kDa) (Sartorius) as described by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). A second SEC was performed on an Äkta prime plus FPLC (Cytiva) ...
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bioRxiv - Genomics 2024Quote: ... The buffer containing the free nuclei was then filtered through a 20μm Minisart nylon filter (Sartorius, Goettingen, Germany) into a 1.5ml Eppendorf and kept on ice while the other samples were processed ...
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bioRxiv - Cell Biology 2022Quote: ... viral particles were isolated by filtering the medium containing them through a 0.45μm filter (16537 from Sartorius stedim biotech). Then ...
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bioRxiv - Genomics 2020Quote: ... Approximately 20 aliquots (100 μl each) from this cell suspension were spotted onto 0.45-μm filters (Sartorius, Göttingen, Germany). These filters were placed onto agar medium H plates and incubated at 30 °C for five days ...
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bioRxiv - Cell Biology 2021Quote: ... Viral supernatants were collected within the 48-hour to 96-hour window and filtered with a 0.45 μm filter (Sartorius).
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bioRxiv - Molecular Biology 2021Quote: ... Triton X-100 and incubation at RT for 60-120 mins and solutions sterile filtered using 0.45 µm Sartopore 2 filters (Sartorius). Triton X-100 inactivated solutions were batch-wise loaded onto a MAb PGT145 immunoaffinity column for capture of ConM and ConS Env trimers ...
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bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500 ...
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bioRxiv - Biochemistry 2022Quote: The NSAR/OSBS and dipeptide epimerase variants were exchanged into D2O using a Vivaspin Turbo 15 centrifuge filter (Sartorius). A 10 mL aliquot of protein was concentrated to 1 mL ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant containing lentiviral particles was collected after 48 h and filtered through a 0.45 μm sterile filter (Sartorius Stedim). Lentivirus supernatants were concentrated with PEG-it™ (SBI ...
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bioRxiv - Neuroscience 2021Quote: ... The filtered supernatant was poured into the optimized VIVASPIN filter unit (No. VS2041, Sartorius Stedim Lab Ltd., Stonehouse, UK) and was centrifuged at 3,000 rpm until reaching less than 1 ml repeatedly for 20–30 min at room temperature (KN-70 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were filtered through a 0.45 μm vacuum filter and concentrated to 70 mL using a VIVAFLOW 100,000 MWCO Hydrosart (Sartorius, Germany). Concentrated supernatants were then subjected to ultracentrifugation at 160,000 x g for 2 hours at 4°C using a Beckman Ti 45 rotor (Beckman ...
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bioRxiv - Neuroscience 2023Quote: ... nasal lavage fluid harvested from the mouse model was filtered through a 0.2-µm syringe filter (Sartorius, Goettingen, Germany) to remove aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 by repeated ultracentrifugation using a Vivaspin 500 filter device with a cutoff of 30 kDa (Sartorius) at 4 °C and afterwards concentrated ...
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bioRxiv - Cell Biology 2023Quote: ... samples were denatured in UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and centrifuged on a 30 kDa cut-off filter device (PALL or Sartorius) and washed thrice with UA buffer and twice with 50 mM ammoniumbicarbonate (ABC) ...
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bioRxiv - Immunology 2023Quote: ... proteins were buffer-exchanged into TN75 (75 mM NaCl, 20 mM Tris HCl pH 8.0) buffer by ultrafiltration with Vivaspin20 filters (Sartorius) of MWCO 100 kDa ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were sonicated at 50% amplitude for 60 s using a sonicating probe (MSE) and the supernatants were passed through a 0.45 μm syringe filter (Sartorius). StrepII-tagged proteins were captured using 1 mL StrepTrap HP column (Cytiva ...
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bioRxiv - Immunology 2022Quote: ... Eluates were subsequently concentrated and buffer exchanged to phosphate-buffered saline (PBS) or TN75 buffer (75 mM NaCl and 20 mM Tris HCl, pH 8.0) using VivaSpin20 filters (Sartorius). Protein concentrations were determined by the Nanodrop.
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bioRxiv - Immunology 2024Quote: ... and ‘lo’ (less than 100 kDa) fractions using 100 kDa filters for 50 ml and 1.7 ml tubes (Vivaspin 20 and 500, Sartorius). Bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... the samples were centrifuged at 17,000 g for 3 min and 150 µl supernatant of each sample was subsequently filtered through a 0.8 µm filter (Sartorius). This filtrate was treated with a mix of Benzonase (50 U ...
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bioRxiv - Cell Biology 2023Quote: ... the obtained labile iron solution was subjected to sterilization by filtering through a 0.22 μm cellulose acetate syringe filter membrane (Sartorius).
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bioRxiv - Cell Biology 2024Quote: ... pollen contained in 5 ml suspension was vacuum-filtrated onto a round polyamide membrane filter (diameter 5 cm, pore size 0.45 µm; Sartorius) and transferred onto 3.5 ml solid (0.25 % phytagel [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2023Quote: ... cell-free media was prepared for the LC-MS run by dilution in 1:1 in acetonitrile at a final volume of 1 ml and passed through 0.2 μm filters (Sartorius). Analysis of TPA ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtrate was then concentrated using a tangential flow filter with a 100 kDa membrane (Viva-flow 50R, Sartorius), followed by ultrafiltration using 100 kDa Amicon Ultra-15 units (Milex ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were resuspended in 200 μL 8 M urea (pH 8.0) and loaded in 30 kD Microcon filter tubes (Sartorius) and centrifuged at 12,000 g for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Microbiology 2023Quote: ... Equal quantities of both bacteria were mixed and spotted onto a cellulose acetate membrane filter (0.2 µm, 25 mm, Huberlab, Sartorius) placed on BHI agar with 500 µM DAP ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... pooled and concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Vivaspin® Turbo 15; Sartorius, UK) prior to being applied to a S75 column pre-equilibrated in 25 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... and the supernatant obtained after centrifugation was filtered using Minisart cellulose acetate syringe filters with a 1.2 µm pore size (Sartorius). Then ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing soluble His-SUMO-GLT25D1/COLGALT1 protein was filtered through a MiniSart GF 0.8 μm filter (Sartorius), loaded onto a 5 mL NiSepharose Excel column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... These enrichment cultures were passed through a 0.22 µm filter to remove the remaining cells (Minisart High Flow PES, Sartorius), and the filtrate was used with the superhost in an agar overlay for viral plaque isolation as described above ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were transferred to new tubes and filtered using Minisart cellulose acetate syringe filters with a 1.2 µm pore size (Sartorius). The filtrate was transferred to ultra-clean 2 mL tubes and 280 µL were collected for nucleic acid extraction using the QIAamp Viral RNA mini kit (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Bioengineering 2021Quote: ... Virus was partially purified using depth filtration and anion exchange chromatography with syringes and small-scale filters (C0SP 23 cm2 [Merck] and Sartobind Q Nano [Sartorius]). Buffer compositions and other parameters were as previously described but scaled proportionately to the product and filter sizes (5) ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were analyzed for pure proteins by 12% SDS-PAGE and fractions containing pure enzymes were pooled and concentrated using Vivaspin concentrators with 30 kDa cut-off filter (Sartorius). Protein concentrations were determined using the corresponding extinction coefficient at 280 nm (ExPASy ...
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bioRxiv - Physiology 2021Quote: ... cells were collected by vacuum filtration on pre-weighed and dried membrane filters (0.2 μm, regenerated cellulose, 47 mm, Sartorius, France). The filters were then washed with 0.9% NaCl and dried at 60 °C under partial vacuum for at least 24 h until a constant weight was achieved ...