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Citations for Sartorius :
351 - 378 of 378 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the buffer was exchanged to 10 mM Tris pH 7.4 using Vivaspin 500 columns with 30000 Dalton cut off (Sartorius Stedim biotech). The samples were centrifuged (12000 g ...
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bioRxiv - Microbiology 2024Quote: ... G3PDH containing fractions as judged by SDS-PAGE and activity measurements were pooled and imidazole was removed using a centrifugal concentrator (10 kDa cutoff, Sartorius, Germany). Protein aliquots were frozen in liquid nitrogen and stored at −80 °C for further use ...
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bioRxiv - Microbiology 2023Quote: ... A sample of 6 liters of air per min was collected during the <10 min exposure on the 47 mm gelatin filter (Sartorius, Germany). Post exposure ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteolytic digestion was carried out according to the FASP protocol32 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) using 30 ug of protein resulting from each fraction ...
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bioRxiv - Microbiology 2024Quote: ... cultured in DMEM (10% FBS and 3% glutamine) were grown to 80-90% confluency in Incucyte® ImageLock 96-well plates (Sartorius) and 700-800 µm scratch was made in cells using an Incucyte® 96-Well Woundmaker ...
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bioRxiv - Microbiology 2024Quote: ... Microbial biomass was collected from 10 litres of water filtered through five 47 mm diameter 0.2–µm polyethersulfone membrane filters (Type 15407, Sartorius Stedim Biotech) using a sterile glass filter holder (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Biochemistry 2020Quote: All decellularization fractions were digested using the filter aided sample preparation (FASP) protocol as previously described32 using 10 kDa molecular weight cutoff filters (Sartorius, Vivacon #VN01H02). Digests were performed for 16 hours in 25 mM ABC pH 8.0 using trypsin at a 1:100 enzyme:protein ratio at 37°C in an oven ...
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500, Sartorius AG, Göttingen, Germany), the samples were then brought to comparable protein concentrations and submitted to the Proteomics Facility of the University of Konstanz for protein mass fingerprinting ...
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bioRxiv - Immunology 2022Quote: ... The formulations were then diafiltrated against 100 x volume of Phosphate Buffered Saline (PBS) through a tangential-flow filtration (TFF) membrane with 10 kD molecular weight cut-offs (Sartorius Stedim Biotech), concentrated to a required concentration ...
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bioRxiv - Microbiology 2020Quote: ... filtered at 0.22 μm and concentrated to 1 mL using a Vivaspin turbo ultrafiltration units (10 kDa MWCO, Sartorius AG, Goettingen, Germany). Samples were further concentrated to 50 μL after five successive washes using Tris 50 mM ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Biophysics 2022Quote: ... The peak fractions were collected and concentrated to <500 µL using a VivaSpin concentrator with 10 kDa cut-off (Sartorius Stedim Biotech). The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: A volume of 25 µl of single-cell suspension was dispensed into each well using an 8-channel electronic Picus® pipette (10–300 µl, #735361, Sartorius, Germany). To evaluate cell viability ...
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bioRxiv - Neuroscience 2024Quote: Purified human A53T aSyn or mouse aSyn was concentrated to 5 mg/mL (347 µM) using a 10 kDa molecular weight cutoff (MWCO) spin filter (Sartorius, Columbus, OH) operated at 4,500 × g ...
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bioRxiv - Neuroscience 2024Quote: Purified mouse aSyn was concentrated to 5 mg/mL (347 µM) using a 10 kDa molecular weight cutoff (MWCO) spin filter (Sartorius, Columbus, OH) operated at 4,500 × g ...
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bioRxiv - Cancer Biology 2024Quote: ... Melanoma cells used for in-vitro experiments and Jurkat E6.1 cells were maintained with RPMI-1640 medium with 10% FBS and 1 mM sodium pyruvate (Sartorius, #03-042-1B). A549 cell line was maintained in DMEM with 10% FBS ...
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... Thirty micrograms of protein resulting from each fraction was subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol 20 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethylphosphine) ...
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bioRxiv - Microbiology 2021Quote: ... dispersed in deionized water with different pH were ultrasonically treated for 10 min at a frequency of 24 kHz (Sartorius labsonic M, Germany). The difference in the hydrodynamic diameter of the nanocomposites was investigated using a Zetasizer Nano-ZS-90 Dynamic Light Scattering (DLS ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteolytic digestion of sample extracts was carried out according to the FASP protocol33 using 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) and loading 10 ug of protein resulting from each fraction ...
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bioRxiv - Biochemistry 2023Quote: Samples were subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol (Wisniewski et al. 2009) with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethyl)phosphine) ...
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bioRxiv - Immunology 2022Quote: ... or monitored in short time periods using a 10 x magnification in an Incucyte®S3 Live-Cell Analysis System (Sartorius, Göttingen, Germany). Image analysis was conducted with the machine learning software ilastik [80] using pixel and object classification to distinguish background from cell nuclei and to determine IRF3-GFP and H2B-mCherry colocalization ...
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bioRxiv - Cancer Biology 2024Quote: ... Thirty μg of protein resulting from each fraction was subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol39 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5mM tris(2-carboxyethyl)phosphine) ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through of the column was concentrated (Vivaspin®, mwco 10 or 30 kDa for single SBD or tandem mutants, Sartorius; ~ 5 mg/ml), dialyzed in buffer A supplemented with 50 % glycerol ...
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bioRxiv - Molecular Biology 2022Quote: ... were mixed together as well as eluates from samples (Pho92-HTP x 2 reps + Pho92-HTP ime4Δ/Δ x 2 reps) before concentration with Vivacon® 500 filtration cartridges (10 kDa MWCO; Sartorius, Cat# VN01H02) to a final volume of 120 μL ...
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bioRxiv - Bioengineering 2023Quote: ... Batch reactors were incubated for 10 days at a mesophilic temperature of 35°C in a Certomat® BS1 incubator (Sartorius Stedim Biotech, Germany) under continuous agitation (145 rpm) ...