Labshake search
Citations for Sartorius :
201 - 250 of 378 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... approximately 4 mg of pulverized tissue was weighed using an ultra-micro balance (CP2P Microbalance, Sartorius GmbH, Goettingen, Germany), packaged into tin capsules ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed once in PBS and kept in 500μL PBS at 4°C in the dark until analysis with an Intellicyt iQue3 Screener (Sartorius). For viability analysis ...
-
bioRxiv - Cell Biology 2023Quote: ... Phase contrast images were acquired every 4 hours over a period of 48 hours using an Incucyte machine (Sartorius). The percentage of cell confluence was calculated using the attached Incucyte®software (Sartorius).
-
bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
-
bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
-
bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtered with Minisart RC4 (pore size 0.20 µm, diameter 4 mm, Sartorius stedim biotech, Göttingen, Germany) and injected into a high-performance liquid chromatography system (LC-10AD ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
-
bioRxiv - Immunology 2024Quote: ... the DAN-G single knockout cells was co-cultured with TCR-T cells at a 4:1 T cell:tumor cell ratio and imaged every 4 hours at 10X magnification using an Incucyte S3 machine (Sartorius).
-
bioRxiv - Biophysics 2024Quote: ... 4 μM of 6xHis-tagged ASF1A1-172 was immobilized on nickel-charged tris-nitriloacetic acid (Tris-NTA) biosensor (Sartorius) for the 90 seconds ...
-
bioRxiv - Biochemistry 2020Quote: Digestion of 10His-SUMO1T95R proteins was performed on 10 kDa-cutoff spin filters (Sartorius) according to a published protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Peak fractions were pooled and concentrated with Vivaspin 20 concentrators (10 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (Cytiva) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 10 mL of virus supernatant was filtered through a 0.45 um filter (Sartorius, 16533) and supplemented with 8µg/ml polybrene (Millipore ...
-
bioRxiv - Microbiology 2022Quote: ... concentrated on a Vivaflow 200 concentration system with a 10 kDa-cutoff membrane (Sartorius). The pH of the concentrated supernatant was adjusted to 8.0 with 100 mM Tris HCl and avidin was added to a final concentration of 1 µg/mL ...
-
bioRxiv - Molecular Biology 2023Quote: Vivacon 500 columns with a 10 kDA Molecular weight cutoff membrane (Sartorius, Göttingen, Germany) were equilibrated with lysis buffer and centrifuged (14,000× g ...
-
bioRxiv - Immunology 2022Quote: ... concentrated via ultra-filtration using Vivaspin 20 centrifugal columns (10 kDa cut-off, Sartorius), and loaded on the SEC column (Superdex 200 26/ 60 ...
-
bioRxiv - Neuroscience 2024Quote: ... and 10 μM ROCKi in Hanks’ Balanced Salt Solution (HBSS) (Sartorius; 02-015-1A)] ...
-
bioRxiv - Microbiology 2024Quote: ... the recovered protein was concentrated using a 10-kDa spin concentrator (Sartorius, Stonehouse, UK). Final protein concentration was determined using a Pierce BCA protein assay (Pierce ...
-
bioRxiv - Biochemistry 2024Quote: ... After fraction concentration on 10-kDa cut-off centrifugal concentrator (nitrocellulose, Vivaspin from Sartorius), contaminants were separated by size exclusion chromatography on a Superdex 200 Increase 10/300 GL (GE Healthcare ...
-
bioRxiv - Cell Biology 2024Quote: ... pooled and concentrated using Vivaspin Turbo at 10 kDa cutoff (Sartorius Stedim Biotech, Göttingen) to a final concentration of 0.4 mg/mL ...
-
bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...
-
bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
-
bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
-
bioRxiv - Neuroscience 2020Quote: ... cells and media were collected and this mixture was centrifuged at 3700xg at 4°C to remove debris and then concentrated by ultrafiltration using Vivaflow 200 (Sartorius). The purified AAV particles were collected after running the viral concentrate through an iodixanol column (Opti-Prep density gradient medium ...
-
bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
-
bioRxiv - Cell Biology 2023Quote: MuSCs were freshly isolated and seeded on 0.2% gelatin-coated 96 well plates at a density of 2,000 cells per well in growing conditions and incubated for 4 days in an IncuCyte SX5 Live-Cell Analysis System (Sartorius) at 37°C and 5% CO2 ...
-
bioRxiv - Microbiology 2023Quote: ... Protein was eluted using similar buffer with 300 mM imidazole and concentrated by centrifugation with 4 kDa (AlgF) or 30 kDa (AlgJ and AlgX) cutoff Vivaspin Turbo centrifugal concentrators (Sartorius). His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare ...
-
bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... Then the samples were moved back and forth every 4 h for a period of 8 h between the blue light source and the IncuCyte reader (Sartorius) to allow the acquisition of the phase and the red channels ...
-
bioRxiv - Microbiology 2024Quote: ... and concentrated by centrifugation at 1500 x g at 4 °C using ultrafiltration devices with a filter cut-off at 100 kDa (Centrisart, Sartorius) [37] ...
-
bioRxiv - Biophysics 2024Quote: ... Proteins were further purified by size exclusion chromatography using Superdex-75 or −200 (Cytiva) at 4°C and concentrated by Vivaspin (Sartorius) concentrators at 4°C (Kar9-Bim1 ...
-
bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 30 mg/ml using Vivaspin 20 concentrators (10 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C.
-
bioRxiv - Biochemistry 2022Quote: ... The solution was concentrated to 70 µL in a 10 kDa cutoff Vivaspin concentrator (Sartorius) and the protein was buffer exchanged with 20 mM HEPES pH 7.4 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... prior to concentration (approximately 10-fold) using a Vivaspin 20 MWCO 100kDa centrifugal concentrator (Sartorius) according to manufacturer’s recommendations.
-
bioRxiv - Cell Biology 2023Quote: ... centrifuged (2000rpm for 10 min) and then concentrated using a Vivacell 100 centrifugal concentrator (Sartorius). MAYV titration was performed as described in Mota & Costa 2020 ...
-
bioRxiv - Cell Biology 2024Quote: ... The eluate was concentrated 10-fold to 50 μL using Vivaspin 500 centrifugal concentrator (Sartorius) with molecular weight cut-off at 10 kDa.
-
bioRxiv - Neuroscience 2024Quote: ... Hybridoma supernatants were screened 10 day later in a flow cytometry assay (iQue, Intellicyt; Sartorius) for binding to CHCHD2 protein captured on beads (3D-Carboxy ...
-
bioRxiv - Plant Biology 2024Quote: ... protein samples were concentrated using Vivaspin centrifugal concentrator with a 10 kDa cut-off (Sartorius). Protein concentrations were determined using a Nanodrop Spectrophotometer (ND1000 ...
-
bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
-
bioRxiv - Cancer Biology 2022Quote: ... Medium containing DMSO or increasing concentration of test compound was added to each well and spheroid growth was monitored for 4 days post-treatment using the IncuCyte® Spheroid Analysis system (Sartorius). Images from 4 days post-treatment and day 0 were analyzed using the IncuCyte® Spheroid Analysis software module ...
-
bioRxiv - Bioengineering 2024Quote: ... Cell debris was removed via centrifugation (5000g, 30 min, 4°C) and filtration of the supernatant via Filtropur 0.2 µm filters (Sartorius, Göttingen, Germany). The soluble protein fraction was then loaded on a HisTrap HP column (GE Healthcare ...
-
bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
-
bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
-
bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
-
bioRxiv - Cell Biology 2024Quote: ... Plates were imaged within a 4-hour interval in a multi-spheroid mode using an Incucyte S3 high-content screening system (Sartorius, Bohemia, NY). Average cyst areas (μm2 ...
-
bioRxiv - Neuroscience 2024Quote: ... 3-4 organoids from each hPSCs line were transferred to a 60 mm culture dish containing 4 mL of pre-warmed Earle’s Balanced Salt Solution (EBSS) (Sartorius; 02-010-1A). The organoids were manually dissected into small pieces and transferred into a new 60 mm dish containing 2.5 mL of Enzymatic dissociation solution [composed of 27.3 U/mL papain solution (Worthington ...
-
bioRxiv - Immunology 2024Quote: ... Assay cells were fixed with 4% paraformaldehyde (PFA) before microsphere uptake readouts (“phagoscore”) were quantified using the iQue Screener Plus (Sartorius, Göttingen, Germany).
-
bioRxiv - Cell Biology 2021Quote: ... fresh medium was supplemented with 1:10 000 IncuCyte® Cytotox Green Reagent (Sartorius, Göttingen, Germany) to determine dead cells ...