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Citations for Sartorius :
301 - 350 of 378 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).
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bioRxiv - Biophysics 2022Quote: BLI analyses were performed at 25°C at an acquisition rate of 5 Hz averaged by 20 using an Octet© BLItz System (FortéBio/Sartorius) with assays were designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Cell Biology 2022Quote: ... individual frames of cells growing under normal culture conditions were acquired every 5 minutes for 24 hours using an IncuCyte ZOOM live-cell analysis system (Sartorius) equipped with a 20 x objective.
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Immunology 2020Quote: ... The EV pellet was resuspended in 2 ml PBS and stored at −80 prior to further concentration using a 5 kDa MW cut-off vivaspin (Sartorius). The protein content of EVs was quantified using Qubit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated-NIH-CoVnb-112 was diluted into assay buffer at 5 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing FKBP12 F36V were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of FKBP12 F36V was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... for a total of 15-20 minutes with trituration every 5 minutes using 200 μl low bind tips (Sartorius, 790101F). Papain was inactivated using an Ovomucoid inhibitor (Worthington Papain Dissociation Kit ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Immunology 2023Quote: ... The cocultures were incubated for 48 hours in a humidified incubator (37 °C, 5% CO2) equipped with an Incucyte (Sartorius) for continuous monitoring of cell proliferation and target cell killing ...
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated NPNA peptides were diluted to 5 μg/mL in kinetics buffer (KB) and immobilized onto streptavidin BLI biosensors (Sartorius); His-tagged rsCSP was diluted to ~1 μg/mL in KB and loaded onto Ni-NTA biosensors ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 104 cells were seeded in each well of an Incucyte® ImageLock 96-well plate (Sartorius, Catalog #: 4379) in 100 μL/well of either complete RPMI or DMEM ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing biotinylated primer bound to monovalent streptavidin were subsequently annealed to a 5’ biotinylated DNA template before binding to streptavidin biosensors (Sartorius). 200 nM of DNA substrates were added in the loading step ...
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bioRxiv - Immunology 2023Quote: ... Mouse antibodies (7.5 μg/mL anti-OVA and 5 μg/mL anti-RBD) were captured on pre-conditioned anti-mouse IgG Fc-capture biosensors (AMC, Sartorius) for 600 s ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The supernatant (EV-depleted HES) was removed and concentrated using a VivaSpin 20 centrifugal concentrator with a 5 kDa molecular weight cutoff (Sartorius). Purified EVs were washed twice with PBS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 x 104 WM852 or 5 x 104 WM793 cells were seeded in a 96-well ImageLock plate (Sartorius, 4379) and allowed to form a monolayer for 24 h prior to the assay ...
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bioRxiv - Microbiology 2024Quote: ... intracellular parasites were isolated by syringe passage with 23-gauge blunt needles (SAI Infusion Technologies) and filtration through a 5 μm membrane filter (Sartorius).
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bioRxiv - Biochemistry 2024Quote: ... The DNA duplex was captured at a concentration of 1.25 µg/ml in BLI Buffer (ELB containing 0.05% Tween-20 and 5 mg/ml BSA) on an Octet® Streptavidin (SA) Biosensor (Sartorius). His-tagged X ...
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bioRxiv - Cell Biology 2024Quote: Blotted membranes were incubated for 1 hr at room temperature in blocking buffer (5% defatted milk/phosphate buffered saline (Sartorius), 0,1% Tween 20 (Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... and then a volume of 1.8 – 4.5 l of filtrate from the pre-filtration was filtered under mild vacuum (<100 mm Hg) through a 0.2 μm isopore polycarbonate filter (Sartorius, Göttingen, Germany) to collect microbial biomass ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... EVs were concentrated to 75 ml by tangential flow filtration (TFF) using two 100 kD Vivaflow 50R cassettes (Sartorius, Goettingen, Germany) run in parallel on a Cole-Parmer Masterflex L/S peristaltic pump operated at 100 rpm ...
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bioRxiv - Synthetic Biology 2022Quote: ... cosmids were concentrated and buffer-exchanged against Phosphate Buffered Saline (PBS) by tangential flow filtration (MWCO 100 kDA; Sartorius Vivaflow 200). Packaged cosmid concentration was analyzed by E ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was filtered through a 0.22μm PVDF membrane filter and concentrated to a volume of 500μl at 3320 xg using 100 kDa molecular weight cut off Vivaspin centrifugal ultrafiltration columns (Sartorius, Stonehouse, UK). The resulting filtrate was stored at −80°C until processed for EV isolation.
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
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bioRxiv - Cell Biology 2023Quote: ... The formulations were then diafiltrated against a 10 × volume of PBS (pH 7.4) through a tangential-flow filtration membrane with a 100 kDa molecular weight cut-off (Sartorius Stedim Biotech), concentrated to the desired concentrations ...
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bioRxiv - Bioengineering 2024Quote: ... then the sample was processed at a constant flux of 100 L/m2/h and collected in a sterile singleuse liquid storage bag (Flexsafe® 2D, Sartorius).
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bioRxiv - Microbiology 2024Quote: ... VLPs in the filtered supernatants were concentrated and smaller metabolites removed by ultrafiltration using a Centrisart ultrafiltration devices with a filter cutoff at 100 kDa (Sartorius, UK). To remove free DNA/RNA molecules ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Plant Biology 2021Quote: ... the pH of the hydroponic culture media of the 5 treatments was measured using a pH meter (PB-10, Sartorius, Germany).
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bioRxiv - Plant Biology 2020Quote: ... Material for analyses was obtained from seeds pre-cooled for 15 min in 5-mL Teflon capsules (Sartorius GmbH, Göttingen, Germany), containing a 10 mm-diameter agate bead ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Microbiology 2021Quote: ... using a centrifugation tube with an insert containing a molecular weight cut-off filter (5 kDa, Sartorius Stedim Biotech, Goettingen, Germany). Each HMW fraction was purified by flushing with 20 ml of water ...
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Immunology 2023Quote: ... the mNeonGreen signals from infected MRC-5 cells were monitored for 1 week using the Incucyte™ Live-Cell analysis system (Sartorius). Additionally ...
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bioRxiv - Bioengineering 2024Quote: On-chip measured affinity of antigen-Ab pairs was confirmed with biolayer interferometry (Supplementary Fig. 5) using an Octet RED96e (Sartorius, Germany). Samples were dispensed into 96-well microtiter plates (655209 Greiner ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2mL Centrifugal filter (Millipore #UFC210024) ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2 ml Centrifugal filter (Millipore ...