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Citations for Sartorius :
251 - 300 of 378 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted by centrifugation (16,000g, 5 min) and the supernatant passed through a 0.22 μm nitrocellulose filter (Sartorius). Proteins were precipitated with 10% trichloroacetic acid (TCA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were placed in the Incucyte SX5 and maintained at 37°C and 5% CO2 (Sartorius, Göttingen, Germany) for detection and automated analysis of cell growth/proliferation ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... samples were clarified using a syringe with a filter cascade (5 µm, 1.2 µm, 0.8 µm, 0.45 µM, Sartorius AG). The cell pellets were not further analysed ...
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bioRxiv - Bioengineering 2024Quote: ... the shake flask culture was centrifuged for 5 min at 110 xg (Model 6K15, Sartorius AG, Göttingen, Germany). The supernatant was discarded and the cell pellet was resuspended in an appropriate volume of medium to generate a stock cell solution with an estimated concentration of 6.6 × 106 cells mL−1 for A and of 2.2 × 107 cells mL−1 for B ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Filtered HES was concentrated using a VivaSpin 20 centrifugal concentrator with a 5 kDa molecular weight cutoff (Sartorius). EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). A second SEC was performed on an Äkta prime plus FPLC (Cytiva) ...
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bioRxiv - Genomics 2020Quote: ... Approximately 20 aliquots (100 μl each) from this cell suspension were spotted onto 0.45-μm filters (Sartorius, Göttingen, Germany). These filters were placed onto agar medium H plates and incubated at 30 °C for five days ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl pH 7.5 by ultrafiltration using 100 kDa molecular weight cut-off (MWCO) spin concentrator tubes (Vivaspin, Sartorius). Protein concentration was then determined using absorbance at 280 nm (ε280 = 14,565 mol−1cm−1 ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.4) and added to 100-kDa molecular mass cutoff Vivaspin 20 centrifugal concentrators (100,000 MWCO, Sartorius AG, Germany). The mixture was centrifuged twice at 2,500 × g for 25 to 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Purified V-ATPase in buffer containing LMNG was concentrated to 1.9 mg/mL with a 100 kDa MWCO VivaSpin 6 centrifugal concentrator at 2000 ×g for 15 min (Sartorius), followed by a VivaSpin 500 concentrator at 12,000 ×g for 2 min.
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bioRxiv - Microbiology 2021Quote: ... 10E7 secreted infectious particles per sample were used) were concentrated using Vivaspin units with 100 MWA cut-of (Sartorius) by centrifugation at 3 000 g for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Then pre-cleared media was concentrated using Vivaflow® 50R cassette with 100 kDa molecular cut-off (VF05H4, Sartorius). Concentrated media was centrifugated at 100000×g for 4 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated EVs were subsequently concentrated to a volume of 100 ul using a 2 ml 100,000 kDa Vivaspin column (Sartorius).
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bioRxiv - Synthetic Biology 2022Quote: Purified NT2RepCT (suspended in 20 mM Tris-HCl pH 8.0 buffer) was concentrated to approximately 100 mg/mL using a Vivaspin centrifugal concentrator (Sartorius) with 10 kDa molecular-weight cutoff.
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bioRxiv - Biochemistry 2024Quote: ... RC-LH1 monomer and dimer bands were buffer exchanged into 50 mM Tris at pH 7.5 with 100 mM NaCl and 0.03% w/v β-DDM by spin concentration with 50,000 MWCO centrifugal concentrators (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: ... The filtrate was then concentrated using a tangential flow filter with a 100 kDa membrane (Viva-flow 50R, Sartorius), followed by ultrafiltration using 100 kDa Amicon Ultra-15 units (Milex ...
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bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further concentrated to ∼1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 1000 ×g.
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bioRxiv - Biochemistry 2024Quote: ... the protein was transferred to a Vivaspin® 6 centrifuge unit with a 100 kDa MWCO PES membrane (Sartorius).
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the protein-RNA complex were concentrated to 400-700 uM with Vivaspin 5-mL centrifugal devices with 10,000 MWCO (Sartorius). Before the measurements a 10% molar excess of RNA was added to saturate the protein as well as 10% v/v D2O ...
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bioRxiv - Biophysics 2023Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Cell Biology 2024Quote: ... pollen contained in 5 ml suspension was vacuum-filtrated onto a round polyamide membrane filter (diameter 5 cm, pore size 0.45 µm; Sartorius) and transferred onto 3.5 ml solid (0.25 % phytagel [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was incubated at 37°C and 5% CO2 and imaged using an Incucyte Live-Cell imager (Sartorius). The red channel used an acquisition time of 400 milliseconds for all replicates and samples ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Microbiology 2024Quote: ... VHH-His (or sACE2-His) (5 µg/ml) diluted into Sartorius’s PBS working buffer were immobilized onto Ni-NTA biosensors (Sartorius), which were pre-equilibrated in the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Cancer Biology 2024Quote: ... and ABTR#2 cells were infected at an MOI of 3-5 with the IncuCyte Nuclight Red (#4476 Sartorius) or IncuCyte Cell Cycle Green/Red Lentivirus Reagent (#4779 ...
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bioRxiv - Synthetic Biology 2024Quote: ... strains were cultivated by fed-batch fermentation in a 5 L bioreactor (BIOSTAT® B-DCU, Sartorius, Goettingen, Germany). All fermentations were carried out in duplicate ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS (Ca+/Mg+) and the scratch was performed with the Incucyte® Cell Migration Kit (Sartorius). The cells were washed carefully twice with PBS (Ca+/Mg+ ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed with PBS (Ca+/Mg+) and the scratch was performed with the Incucyte® Cell Migration Kit (Sartorius). The cells were washed carefully twice with PBS (Ca+/Mg+ ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Biophysics 2021Quote: ... 0.1 mM EDTA [pH 7.4]) and added to the 100-kDa molecular mass cutoff Vivaspin 20 centrifugal concentrators (100,000 MWCO, Sartorius AG, Germany). The mixture was centrifuged twice at 2,500 × g for 25 to 30 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: FITC: AcmJRL was diluted in Na2CO3-buffer (100 mM, pH 9.3) and concentrated (Vivaspin, Sartorius Stedim Biotech GmbH, 10,000 MWCO) to yield a final protein concentration of 78 μM (1.8 mg in 1.5 mL) ...
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bioRxiv - Microbiology 2022Quote: ... was concentrated to 7 mL using either 100 kDa cutoff centrifugal filters (Amicon) or a tangential flow filtration device with a 100 kDa cutoff filter (Vivaflow 50R, Sartorius). Concentrated Bt CM was then ultracentrifuged at 135,000 x g for 2 hours to pellet crude Bt OMVs ...
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bioRxiv - Synthetic Biology 2020Quote: ... 20 min) followed by cross-flow filtration of the supernatant using a 100 kDa filtration cassette (Hydrosart, Sartorius AG, Germany). EPS was precipitated by slowly pouring the concentrated fermentation supernatant into two volumes of isopropanol ...
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated with increasing concentrations (12.5-100 μM) of TLR agonists in complete medium in the presence of IncuCyte® Cytotox Red (Sartorius), following the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: The sterile filtered culture/fecal supernatant was concentrated using centrifugal filters Centrisart with a filter cut-off at 100 kDA (Sartorius) by centrifugation centrifuged at 1,500 x g at 4°C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Microbiology 2023Quote: ... Flow through of the 0.22 µm filters was subsequently concentrated using tangential flow filtration with a 100 kDa cassette (Sartorius Stedim). The concentrated samples were stored at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: The sterile filtered fecal supernatant was concentrated using Centrisart centrifugal filters with a filter cut-off at 100 kDA (Sartorius) by centrifugation at 1,500 x g at 4 °C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Microbiology 2024Quote: The sterile filtered fecal supernatant was concentrated using centrifugal filters Centrisart with a filter cut-off at 100 kDA (Sartorius) by centrifugation centrifuged at 1,500 x g at 4°C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Microbiology 2024Quote: ... and concentrated by centrifugation at 1500 x g at 4 °C using ultrafiltration devices with a filter cut-off at 100 kDa (Centrisart, Sartorius) [37] ...