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Citations for Sartorius :
251 - 300 of 1075 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Biochemistry 2024Quote: ... and appropriate elution peak fractions from multiple runs were combined and concentrated using a protein concentrator (Sartorius, Vivaspin, 30,000 MWCO) to obtain a final sample of ∼300 μL at ∼30 µM concentration.
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were filtered on a glass fiber-pre-filter combined with a 0.45-µm syringe filter (Sartorius Technologies, Germany). Next ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the antagonistic antibodies were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (PBS ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Biochemistry 2020Quote: ... bengalensis protein hydrolysates (BBPH) were subjected to centrifugal ultrafiltration using 3kDa cut-off membrane filtration unit (Vivaspin20, VS2091, Sartorius AG, Goettingen, Germany) to separate the low molecular peptide fraction (< 3kDa) ...
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Microbiology 2023Quote: ... Fc-tagged RBD proteins (20 μg/mL) were captured on the protein A biosensor followed by 30 s of baseline run in 1x kinetics buffer (Sartorius Cat#: 18-1105). The biosensors were blocked with a control Fc protein (Jackson ImmunoResearch Cat# ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). The recombinant proteins were further purified by ion exchange and gel filtration chromatography with ÄKTAprime plus system ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). For gel filtration ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Immunology 2024Quote: ... Supernatants were quantitated for antibody concentrations by bio-layer interferometry (BLI) on an Octet Red 96 (Sartorius) using protein A biosensors and a human IgG1 isotype control antibody as a standard ...
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bioRxiv - Immunology 2024Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Immunology 2024Quote: ... Antibodies were diluted to a final concentration of 20 ug/mL before being immobilized on ProteinA tips (Sartorius) for 600 s ...
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bioRxiv - Immunology 2021Quote: Affinity measurements for VHH-huFc antibodies were performed using biolayer interferometry (BLI) using an Octet 384 Red system (Sartorius). Measurements were conducted in 10 mM phosphate (pH 7.4) ...
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bioRxiv - Bioengineering 2022Quote: ... The five fermentation experiments to produce a monoclonal antibody were all conducted in controlled automated miniaturized bioreactor (ambr250) systems (Sartorius AG, Germany) for 14 days ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was prepared fresh and was filtered with a 0.2 μm syringe filter (Sartorius) before each experiment.
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bioRxiv - Microbiology 2023Quote: ... The pellet was discarded and the supernatant was filtered through a 0.45 μm pore membrane (Sartorius) twice in order to obtain cell-free supernatant ...
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bioRxiv - Biophysics 2023Quote: ... Lysate was filtered (5µm filter, Sartorius) prior to binding to Streptactin Superflow high-capacity resin (IBA Lifesciences ...
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bioRxiv - Cell Biology 2022Quote: ... medium was changed to remove Ri and the plate was placed in Incucyte S3 live imaging system (Sartorius) and imaged every 2 hours for 50 hours using 10x objective taking 16 frames per well ...
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bioRxiv - Molecular Biology 2023Quote: ... was added at 1:1000 dilution and live cell imaging was performed on the Incucyte S3 System (Sartorius). Apoptosis was calculated using the S3 Live Cell Analysis Instrument (Sartorius ...
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bioRxiv - Cancer Biology 2023Quote: ... media was changed and live-cell timelapse microscopy of treated cells was performed in an Incucyte SX5 (Sartorius). Images were taken every 30 minutes in the phase ...
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bioRxiv - Cell Biology 2019Quote: ... APC was concentrated using Vivaspin concentrators (Sartorius), ultra-centrifuged (280.000 x g ...
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bioRxiv - Microbiology 2020Quote: ... Nitrocellulose membrane was purchased from Sartorius (Germany). Fiberglass pads ...
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bioRxiv - Cancer Biology 2022Quote: ... growth was tracked on Incucyte S3 (Sartorius) and CellTiterGlo (CTG ...
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bioRxiv - Genetics 2024Quote: ... Supernatant was transferred to Flexboy bag (Sartorius) and subjected to tangential flow filtration (TFF ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was transferred to Flexboy bag (Sartorius) and subjected to tangential flow filtration (TFF ...
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bioRxiv - Bioengineering 2022Quote: Acidity was measured with a pHmetrer (Sartorius). Dissolved solids were measured with a brixometer (Arcano ...
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bioRxiv - Plant Biology 2020Quote: ... The root growth was measured using ImageJ (NIH, USA) and fresh weight was measured using a microbalance (Sartorius, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was determined as percent confluence from phase images and was analyzed by IncuCyte image analysis software (Sartorius). For cell death assays ...
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bioRxiv - Microbiology 2020Quote: ... with buffer B Purity was checked via SDS-PAGE with Coomassie Staining and concentration was performed using Vivaspin Turbo apparatus (Sartorius) with a 5 kDa molecular weight cut-off.
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bioRxiv - Cell Biology 2019Quote: ... KAP3A was concentrated using a Vivaspin concentrator (Sartorius), ultra-centrifuged (280.000 x g ...
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bioRxiv - Molecular Biology 2022Quote: ... Buffer exchange was accomplished using Vivaspin concentrators (Sartorius). Protein purity was assessed by SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Biophysics 2022Quote: ... was loaded to a streptavidin biosensor tip (Sartorius) for 120 s ...
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bioRxiv - Cancer Biology 2022Quote: ... The plate was then inserted inside IncuCyte (Sartorius) instrument and the co-culture was followed for 3 days acquiring images every hour ...
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bioRxiv - Biophysics 2023Quote: ... The plate was imaged with the Incucyte (Sartorius) taking 9 images per well every 4 hours and phase masking algorithms were applied to determine cell confluence using the Incucyte software v.S3 2019A.
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bioRxiv - Molecular Biology 2023Quote: Relative proliferation was measured using an Incucyte (Sartorius) instrument ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis was quantified using the IncuCyte platform (Sartorius). OLN-93 cells were seeded (7000 cells/well ...
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bioRxiv - Biophysics 2023Quote: This was performed using an Octet Red385 (Sartorius). Streptavidin coated biosensors were loaded with biotinylated TREK2 at 50 nM in 200mM KCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferation was measured using the Incucyte platform (Sartorius). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Data analysis was performed using ForeCyt software (Sartorius) by gating successively on cells ...
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bioRxiv - Microbiology 2023Quote: ... was diluted with the 10X Kinetic Buffer (Sartorius) to 0.5 µg/mL and immobilized to Octet® HIS1K Biosensors ...