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Citations for Sartorius :
101 - 150 of 1075 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then concentrated to 0.5 ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius, MWCO 50 kDa).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Neuroscience 2023Quote: Bio-Layer Interferometry (BLI) for compound/protein direct binding was performed using the Octet RED384 system (Sartorius BioAnalytical Instruments) and biotinylated SYK-tSH2 protein (SYKA-c022 cleaved/biotinylated ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Biochemistry 2024Quote: ... Human TLR3-FC (Acro Biosystems) was diluted to 0.01 mg/mL in kinetics buffer and loaded onto Protein A tips (Sartorius). The cell assay constructs were diluted to 10 μM in kinetics buffer and their association was measured for 300 s ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing soluble His-SUMO-GLT25D1/COLGALT1 protein was filtered through a MiniSart GF 0.8 μm filter (Sartorius), loaded onto a 5 mL NiSepharose Excel column (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... we used IncuCyte Caspase-3/7 Green Reagent for apoptosis (Sartorius). Crystal violet staining assay was used to assess the relative number of cells as previously described [36] ...
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bioRxiv - Immunology 2022Quote: ... and quantified on the iQue 3 VBR using Forecyt software (Sartorius). Phagocytic scores were calculated by multiplying the percentage of bead positive cells by the bead fluorescence GMFI of bead positive cells and dividing by 100,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... immediately followed by the addition of caspase 3/7 dye (Sartorius) at 0.5uM ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting clarified supernatant was loaded onto Vivaspin protein concentrator columns with a 30 kDa molecular weight cut-off (Sartorius) and centrifuged at 4,000 x g and 4 °C until the sample was concentrated by a factor of 100 ...
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bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
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bioRxiv - Synthetic Biology 2022Quote: The binding kinetics of antibodies to RBD was measured by BLI on an Octet-R2 (Sartorius). The his-tagged RBD variants were loaded onto Ni-NTA biosensors from 10 ug\ml solution in kinetic buffer (PBS ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated using a Vivaspin concentrator (Sartorius) with a half lower MWCO than the size of the protein being purified ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Microbiology 2019Quote: ... Each mixture containing 100μg proteins was loaded onto a 10 kDa cut-off spin column (Vivacon 500, Sartorius AG, Goettingen, Germany). The lysate was adjusted to 8M urea by centrifuging to remove SDS and low-molecular-weight material ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG) and passed through a spin-out column of Bio-Gel P-6 equilibrated with the protein storage buffer (0.1 M Hepes-HCl ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then loaded into a Vivaspin 3 KDa MWCO (Sartorius) and washed thrice with 200 µL of MS-grade H2O ...
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bioRxiv - Cell Biology 2022Quote: ... ECs and SMMCs were cultured in BioAMF-3 (01-196-1A, Sartorius).
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bioRxiv - Neuroscience 2023Quote: ... the IncuCyte Caspase-3/7 Red apoptosis reagent (1.5 µM; #4704, Sartorius) was supplemented to the cultures ...
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... 3 L of water were pre-filtered through the 5 μm filter (Sartorius). The flow-through was further filtered using the 0.2 μm filter (Sterivex) ...
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bioRxiv - Developmental Biology 2020Quote: ... and sterile gelatin filters (3 μm pores and 80 mm diameter, Sartorius, Germany)[7,14] ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Immunology 2021Quote: ... Protein production at pilot scale for nonclinical trial material was archived in 500L Bioreactor (BIOSTAT STR®500, Sartorius Stedim Biotech, Göttingen, Germany) using a fed-batch process using PowerCHO-2 medium as basal medium ...
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bioRxiv - Biochemistry 2021Quote: ... All protein samples were concentrated using Vivaspin centrifugal concentrators (Sartorius). Denaturing gel electrophoresis was performed using NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Then proteins were transferred into ultrafiltration tubes (10kDa, Sartorius AG) and washed three times with 50 mM NH4HCO3 at 14000g for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Immunology 2024Quote: ... Experiments were performed using Octet R2 Protein Analysis System (Sartorius) and affinity was calculated using Octet Analysis Studio Software ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, cat #4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by ultrafiltration at 3-bar with a 100 kDa filter (Sartorius, Goettingen, Germany). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1µg/µl of CpG and 0.9ul of caspase-3/7 dye (Sartorius, 4440) were added to the well for 72hrs incubation in the SpectraMax M5 and Lmax microplate reader ...