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Citations for Sartorius :
251 - 300 of 783 citations for T cell leukemia lymphoma protein 1B TCL1B Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Microbiology 2020Quote: ... The protein was loaded on a HiLoad 16/600 Superdex 200 pg column pre-equilibrated with storage buffer and the fractions containing pure Rel protein were collected and concentrated in a Vivaspin turbo 15 (Sartorius). The purity of protein preparations and the absence of RNA were assessed by SDS-PAGE and on a 1% agarose gel (in 1 × TAE buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Immunology 2021Quote: ... Purified S proteins were exchanged to storage buffer and then filtered through 0.22 um filter (Sartorius Stedim Biotech, Göttingen, Germany). The concentration of purified protein was determined by ELISA ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Immunology 2022Quote: KD measurements of the Pertuzumab and Trastuzumab IgMs to their Her2 antigen (Cat: 10004-HCCH, SinoBiological) were performed by first immobilizing them on Protein L (Cat: 18-5085, Sartorius) and CaptureSelect™ Biotin Anti-IgM Conjugate (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were filtered on a glass fiber-pre-filter combined with a 0.45-µm syringe filter (Sartorius Technologies, Germany). Next ...
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bioRxiv - Biochemistry 2024Quote: ... and appropriate elution peak fractions from multiple runs were combined and concentrated using a protein concentrator (Sartorius, Vivaspin, 30,000 MWCO) to obtain a final sample of ∼300 μL at ∼30 µM concentration.
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bioRxiv - Molecular Biology 2024Quote: ... The resulting clarified supernatant was loaded onto Vivaspin protein concentrator columns with a 30 kDa molecular weight cut-off (Sartorius) and centrifuged at 4,000 x g and 4 °C until the sample was concentrated by a factor of 100 ...
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Molecular Biology 2020Quote: Live imaging of mammalian cells was performed using the IncuCyte S3 live cell analysis system (Sartorius) housed within a 37°C ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... Images of GFP-positive cells were obtained with Incucyte® S3 Live-Cell Analysis software (Sartorius).
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bioRxiv - Microbiology 2022Quote: ... Time-lapse live-cell imaging was performed using Incucyte® S3 live-cell imaging system (Sartorius) housed in a humidified incubator kept at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cell proliferation was tracked in real-time using the Incucyte® Live-Cell Analysis System (Sartorius) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... cells were imaged for 24 h using the IncuCyte® S3 Live-Cell Analysis System (Sartorius) at 20 min intervals ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell growth was monitored for 48h after treatment using Incucyte® Live-Cell Analysis System (Sartorius) equipped with a FITC filter allowing the detection of cleaved caspase 3/7 leading to the cleavage of DEVD peptide and the release of green fluorescence ...
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by quantification of GFP-expressing infected cells using a live cell microscope (Incucyte SX5; Sartorius) to obtain the number of focus forming units (FFU ...
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bioRxiv - Microbiology 2023Quote: ... Cultivation was performed in cell culture plates (24 well for suspension cells, Sartorius AG, Göttingen, Germany) in an anaerobic tent (Coy Laboratory ...
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bioRxiv - Cancer Biology 2023Quote: ... and live cell imaging was captured using the IncucyteⓇ S3 Live-Cell Analysis System (Sartorius, Germany). Images were taken every 6 hours at 10x objective ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were imaged and analyzed in an Incucyte S3 live-cell analysis system (Sartorius, Göttingen, Germany), using the adherent cell-by-cell module to assess phase contrast and green and red fluorescence intensities.
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bioRxiv - Cancer Biology 2024Quote: ... cell confluency was determined using an IncuCyte® SX1 live-cell imaging system (Sartorius, Göttingen, Germany) every 4 hours over a 72-hour period to monitor cell growth continuity ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell confluence was monitored over time using imaging with the Incucyte Live-Cell Analysis System (Sartorius). Doublings per day were calculated by fitting the exponential growth equation to proliferation curves using GraphPad Prism 10.
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bioRxiv - Immunology 2024Quote: ... The adherent target cells were then analyzed using the IncuCyte SC5 Live-Cell Analysis System (Sartorius). Plates were scanned using a 10x objective using phase contrast channel ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG) and passed through a spin-out column of Bio-Gel P-6 equilibrated with the protein storage buffer (0.1 M Hepes-HCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... native Tau was purified from the cell extract by sequential anion exchange and phosphocellulose chromatography as previously described.69 The Tau protein eluate was dialyzed against water and concentrated with Vivaspin® (15R, 2,000 MWCO, Sartorius, UK). Protein concentrations were determined by a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2021Quote: ... Live cell imaging and IncuCyte (Sartorius) proliferation assays were performed as previously described (Hastings et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... and Incucyte live cell imaging (Sartorius). Phosphatidylserine exposure was analyzed on a flow cytometer after staining cells with Fluorescent conjugated Annexin V (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... the Incucyte cell culture microscope (Sartorius) was used ...
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bioRxiv - Immunology 2021Quote: ... Infections were monitored in live cells via GFP expression using the Incucyte live-cell analysis system (Sartorius) for 24-72h ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... cells were treated with Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling (Sartorius, 4717) at time of experiment seeding for a final concentration of 1:750 ...
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bioRxiv - Cell Biology 2024Quote: ... and the cells were subsequently imaged over a timecourse using an Incucyte S3 Live-Cell imager (Sartorius). Pyroptosis was measured based on a cell-by-cell classification analysis that quantified the percentage of green positive cells out of the total number of cells imaged.
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bioRxiv - Bioengineering 2023Quote: ... The serum-free suspended cells were cultured in microbioreactor (Ambr® 15 cell culture bioreactor; Sartorius, Germany) to determine the specific productivity (Qp ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell growth was analyzed for percent plate coverage with IncuCyte S3 Live Cell Analysis System (Sartorius). Pictures of each well were acquired every 3 hours for 7 days.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then seeded in 96-well plates in cell growth media containing Annexin V Dye (Sartorius). Cells were imaged by real-time fluorescence microscopy (IncuCyte ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell migration was monitored using time-lapse microscopy (IncuCyte Live Cell Analysis Systems, 4x objective lens, Sartorius) with an interval of 3 h during 21 h ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the antagonistic antibodies were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... bengalensis protein hydrolysates (BBPH) were subjected to centrifugal ultrafiltration using 3kDa cut-off membrane filtration unit (Vivaspin20, VS2091, Sartorius AG, Goettingen, Germany) to separate the low molecular peptide fraction (< 3kDa) ...
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).