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Citations for Sartorius :
151 - 200 of 783 citations for T cell leukemia lymphoma protein 1B TCL1B Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were concentrated with Vivaspin 20-mL centrifugal devices with 5,000 or 10,000 MWCO (Sartorius) and buffer-exchanged into NMR buffer over PD-10 gel-filtration columns (GE-healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Protein was pooled and concentrated with a Vivaspin 30 kDa molecular weight cut-off centricon (Sartorius) and loaded onto a buffer A equilibrated S6 Increase 3.2/300 (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Protein-containing fractions were pooled and concentrated in a Vivaspin 20 centrifugal concentrator (50000 MWCO; Sartorius). Purified complexes were analyzed by spectrophotometry (Nanodrop ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... followed by protein concentration using vivaspin centrifugal concentrators columns (MWCO 3.0 kD, Sartorius GmbH, Göttingen, Germany). Protein contents were analyzed according to Bradford using Rotiquant (Roth ...
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bioRxiv - Biochemistry 2023Quote: ... C-terminally biotinylated SKP1-FBXO22 fusion proteins (WT or C326A) were immobilized onto SA biosensors (Sartorius) to a response level of 1 nm ...
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bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated on a 3-kDa cut-off centrifugal concentrator (nitrocellulose, Vivaspin from Sartorius), and the buffer was exchanged for 25 mM Tris/HCl pH 7.6 ...
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bioRxiv - Cell Biology 2024Quote: ... This recombinant protein was biotinylated for further assessment using the avidin-utilizing Octet R2 (Sartorius, Germany).
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bioRxiv - Cancer Biology 2021Quote: Cell death and cell confluence were determined using live-cell imaging in the IncuCyte Zoom and S3 (Sartorius). For cell confluence 50×103 cells were seeded in Matrigel-coated 6-well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation assays and live-cell imaging were performed using the Incucyte SX5 Live-Cell Analysis System (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to single-cell cloning on the CellCelector (Sartorius) into 96-well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... where 500-1,000 cells were added per well and cell growth monitored using an Incucyte S3 Live-Cell Analysis Instrument (Sartorius). For experiments performed with dox-inducible shRNA expression clones ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was buffer exchanged into PBS using a Vivacon 500 concentrator (Cat: VS0122, Sartorius Stedim). For the generation of human ACE2-hFc and SARS-CoV-2 RBD-mFc recombinant proteins ...
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bioRxiv - Immunology 2021Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were run on Superdex S200 size-exclusion column and concentrated with Vivaspin devices (Sartorius).
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Molecular Biology 2021Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Bioengineering 2022Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Immunology 2022Quote: The Octet quantification assay for protein expression level was performed on an Octet 96 Red system (Sartorius). Harvested cell media were centrifuged and cell supernatants were prepared in a 96-well plate with S-2P or S-6P standards diluted in media from 20 μg/mL to 0.3125 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... protein was eluted and concentrated to 500 μl using a Vivaspin6 10,000 MVCO centrifugal concentrator (Sartorius, Germany). The concentrated sample was subjected to size exclusion chromatography using an Äkta FPLC UPC-900 liquid chromatography system (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture of peptides was separated from large proteins by centrifugation on Vivaspin 500 concentrators (Sartorius, Germany) with a PES membrane resistant to high concentrations of salts and detergents (10 kDa molecular weight cut-off) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins were separated on denaturing SDS-PAGE and blotted onto nitrocellulose membrane filters (Sartorius). The membranes were blocked and incubated with the primary antibodies overnight at 4°C with gentle agitation ...
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bioRxiv - Cancer Biology 2024Quote: ... All glioma lines were stably transduced with a lentivirus expressing nuclear red-fluorescent protein (NucLight Red, Sartorius) or with lentivirus expressing green-fluorescent protein and luciferase for imaging purposes.
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bioRxiv - Biophysics 2024Quote: ... The labeled protein was concentrated to the desired concentration using Vivaspin Turbo 4 10000 MWCO concentrators (Sartorius). The protein concentration was determined with the formula to account for the absorption of fluorescein at 280 nm (https://www.aatbio.com/resources/correction-factor/fluorescein).
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bioRxiv - Immunology 2024Quote: ... 10% FBS was added to the cells and real-time cell death analysis was performed using IncuCyte S3 Live-Cell Analysis instrument (Sartorius).
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bioRxiv - Cancer Biology 2024Quote: ... and cell counts per image were analyzed using the Incucyte® Cell-By-Cell Analysis Software Module (Sartorius BA-04871). For migration assays ...
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bioRxiv - Cell Biology 2024Quote: ... Cell growth was measured using Incucyte software that determined cell numbers in each well of the plate (Cell-by-Cell, Sartorius). Rates of growth were uniform from 24-40 hrs of culture ...
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bioRxiv - Microbiology 2024Quote: ... cells were imaged in the Incucyte SX5 Live-Cell Analysis System (Sartorius). Cell confluence and the percentage of GFP-positive area were quantified automatically with the Incucyte Analysis software ...
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bioRxiv - Microbiology 2024Quote: ... cells were imaged in the Incucyte SX5 Live-Cell Analysis System (Sartorius). Cell confluence and the percentage of GFP-positive area were quantified automatically with the Incucyte Analysis software ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transferred to the Incucyte S3 Live-Cell Analysis System (Sartorius) where cell count and confluency was determined ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were grown in an IncuCyte Zoom live cell imager (Essen/Sartorius) with images recorded every 4 h for 6 d after addition of the drug ...
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bioRxiv - Immunology 2023Quote: ... and cells were monitored with the IncuCyte live-cell imaging system (Sartorius) for up to 4 days ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated in the Incucyte live cell analysis instrument (Sartorius) and green fluorescence and phase images were captured every 3 hours for 48 hours total ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns (molecular weight cut-off of 30 kDa; Sartorius) and centrifuged at 4,000 x g at 4 °C until the supernatant was 100-fold concentrated ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was filtered with a 0.22 µm filter and concentrated using Vivaspin 15R 10K centrifugal filters (VS15RH01, Sartorius). Protein was then loaded onto a Superdex 200 column and 2 mL fractions were collected ...
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bioRxiv - Biochemistry 2022Quote: ... protein solutions were concentrated to about 12 mg ml-1 using a centricon centrifugal filter unit (Sartorius Stedim). The protein concentration was estimated by measuring the absorbance at 280 nm.
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Microbiology 2021Quote: ... CV30 or CR3022 IgG at 10ug/mL was loaded onto pre-hydrated Protein A biosensors (Pall FortéBio/Sartorius) for 150 s followed by a 60 s baseline ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 30 μg of total protein was loaded into 30-kDa-cutoff Vivacon 500 ultrafiltration spin columns (Sartorius). Samples were digested for 4 h on a filter with 1:50 Lys-C (Wako ...
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bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and concentrated to 10 mg/ml using 10 kDa MWCO spin concentrators (Sartorius). Protein purity and identity were confirmed by detection of a single band by Coomassie-stained SDS-PAGE and by LC-MS/MS to confirm the expected sequence.
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bioRxiv - Immunology 2023Quote: ... and 37.7H IgG were diluted to concentrations of 10 µg/mL and loaded onto Protein A sensors (Sartorius) to a binding signal of 1.0 nM ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Cell Biology 2023Quote: ... Cleaved protein was eluted in fresh cleavage buffer and concentrated to 0.5 mL using a centrifugal concentrator (Sartorius). Protein concentration and quality was determined by NanoDrop® ND-1000 spectrophotometer (Labtech ...
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bioRxiv - Immunology 2024Quote: Antibody affinities were determined by biolayer interferometry using an Octet Red96 (Sartorius). HIS1K biosensors (Sartorius ...
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bioRxiv - Cancer Biology 2020Quote: ... For analysis of cell death with the Incucyte live-cell analysis system (Sartorius), Yoyo3 iodide viability die was used at 1/8000 final concentration and images were acquired ...
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bioRxiv - Immunology 2022Quote: Cell death was analyzed using an IncuCyte Live Cell Imager (Sartorius, Goettingen, Germany) at the Genome Engineering and iPSC Center at Washington University ...
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bioRxiv - Cancer Biology 2022Quote: For cell death analysis using the Essen IncuCyte Live-Cell imaging system (Sartorius), cells were plated at a density of 5,000 cells per well in black 96-well plates (Corning ...