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Citations for Sartorius :
201 - 250 of 409 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The filtered supernatant was ultrafiltered and concentrated using tangential concentrator and PES membrane cassette (pore size, 100kDa, Sartorius) and ultracentrifuged at 150,000g for 1.5hr at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... into 20 mM Tris / 500 mM NaCl / pH 8.3 using Sartocon ECO (30 kDa MWCO, 0.70 m²) PES membranes (Sartorius). Buffer exchanged supernatants were filtered using 0.8 + 0.45 µm Sartopore 2 filters (Sartorius ...
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bioRxiv - Microbiology 2021Quote: ... 100 ml of collected water was filtered through 0.22 μm mixed cellulose esters membrane filters (Sartorius, Göttingen, Germany) to capture bacteria ...
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bioRxiv - Synthetic Biology 2023Quote: ... and track-etched polycarbonate membranes (25 mm diameter, 0.2 μm pore size) (Sartorius 23007 or Whatman Nuclepore 10417006) were placed on the filter surface ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed solution was finally concentrated by centrifugation through a Vivaspin Turbo 4 membrane filter (MWCO 10,000; Sartorius) at 3,500 × g for 30-45 min at 4°C.
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bioRxiv - Bioengineering 2024Quote: ... The lysate was clarified by centrifugation at 10,000 g for 45 minutes and filtering through a 0.2 μm membrane filter (Cat # 17823, Sartorius). Mucinase was purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biophysics 2024Quote: ... The sample was then concentrated to 13.5 mg/ml using Vivaspin 500 concentrators with 50,000 molecular weight cutoff (MWCO) PES membranes (Sartorius) on a tabletop centrifuge at 4 °C.
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0 was concentrated to 10 mg.mL-1 using 10 kDa molecular weight cut-off spin filter (Vivaspin® Turbo 15; Sartorius, UK) and screened using commercially available screens ...
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bioRxiv - Bioengineering 2024Quote: ... The solubilised COD was measured in the liquid phase after centrifugation at 1200 relative centrifugal force (RCF) and subsequent vacuum filtration through a 0.2 µm cellulose-acetate filter (Sartorius AG, Göttingen, Germany). The COD was determined using the Spectroquant COD kit (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 10-kDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... the iodixanol was removed from the AAV vector containing fraction by 3 rounds of diafiltration using Vivaspin 20 ultrafiltration devices (100’000 MWCO, PES membrane, Sartorius) and 1x phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtrate was then concentrated using a tangential flow filter with a 100 kDa membrane (Viva-flow 50R, Sartorius), followed by ultrafiltration using 100 kDa Amicon Ultra-15 units (Milex ...
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bioRxiv - Cell Biology 2023Quote: ... the obtained labile iron solution was subjected to sterilization by filtering through a 0.22 μm cellulose acetate syringe filter membrane (Sartorius).
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bioRxiv - Microbiology 2024Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was transferred to a Vivaspin® 6 centrifuge unit with a 100 kDa MWCO PES membrane (Sartorius).
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bioRxiv - Molecular Biology 2021Quote: ... concentrated in a Vivaspin 20 MWCO 10 filter (Sartorius) to 4.8 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with a Vivaspin 6 (10 kDa MWCO) (Sartorius), flash-frozen by liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... a VivaSpin centrifugation filter (10 kDa cut-off, Sartorius), followed by an Amicon ®Ultra centrifugation filter (3 kDa cut-off ...
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bioRxiv - Biochemistry 2023Quote: ... and concentrated by Vivaspin Turbo (Sartorius, 10 kDa MWCO) centrifugation devices at 4,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... samples were filtered using a 10 KDa filter (Sartorius Vivaspin® 2 Centrifugal Concentrator Polyethersulfone ...
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation for 40 minutes at 3000 g and the phage-containing supernatant was filtered twice (Sartorius MinisartTM 0.2 µm) to obtain a phage stock ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was collected through centrifugation at 15000 × g and filtered using Minisart Hydrophilic 0.45-µm filters (Sartorius Stedim Biotech, Göttingen, Germany). The crude enzyme solution was purified using Ni-affinity chromatography (cOmplete His-Tag ...
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bioRxiv - Physiology 2021Quote: ... cells were collected by vacuum filtration on pre-weighed and dried membrane filters (0.2 μm, regenerated cellulose, 47 mm, Sartorius, France). The filters were then washed with 0.9% NaCl and dried at 60 °C under partial vacuum for at least 24 h until a constant weight was achieved ...
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bioRxiv - Immunology 2020Quote: ... HLA-E refolds were filtered through 1.0μm cellular nitrate membranes to remove aggregates prior to concentration by the VivaFlow 50R system with a 10kDa molecular weight cut-off (Sartorius) and subsequent concentration in 10kDa cut-off VivaSpin Turbo Ultrafiltration centrifugal devices (Sartorius) ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Cell Biology 2023Quote: ... Cell-free supernatants were concentrated to 1 mL by ultrafiltration through a membrane with a molecular weight cut-off of 30 kDa (VivaSpin 20, 30,000 MWCO PES (Sartorius, VS2022)) at 4 °C and 4,000 ˗ g ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The supernatant collected from these samples were filter sterilised using 0.20 -micron membrane filter (Minisart, Sartorius Stedim Biotech GmbH, Germany) to obtain cell-free spent media ...
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bioRxiv - Microbiology 2024Quote: ... intracellular parasites were isolated by syringe passage with 23-gauge blunt needles (SAI Infusion Technologies) and filtration through a 5 μm membrane filter (Sartorius).
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bioRxiv - Molecular Biology 2024Quote: ... the cells were pelleted by centrifugation at 5250 × g for 30 minutes and the supernatant was collected and filtered through a 0.22 μm PES membrane (Sartorius®). The BsAbs were purified from the supernatant utilising a 5 mL HisTrap™ excel column (Cytiva™) ...
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bioRxiv - Biochemistry 2024Quote: ... The aptamer was additionally purified using centrifugation in 5x-excess of the buffer using Vivaspin 500 polyestersulfone membranes with 3 kDa cutoff (Sartorius Stedium Lab Ltd ...
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bioRxiv - Cell Biology 2024Quote: Blotted membranes were incubated for 1 hr at room temperature in blocking buffer (5% defatted milk/phosphate buffered saline (Sartorius), 0,1% Tween 20 (Sigma)) ...
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bioRxiv - Cell Biology 2022Quote: ... monocytes were resuspended in PBS and labelled with Incucyte Cytolight rapid green or red reagent for live cell cytoplasmic labelling (0.750 µM, Sartorius, Cat # 4705 and Cat # 4706) for 20 min at 37°C ...
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bioRxiv - Systems Biology 2021Quote: ... filtered through a 10 kDa MWCO spin filter (Vivaspin500; Sartorius), and quantified by NanoDrop OneC.
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bioRxiv - Biophysics 2021Quote: ... using Vivaspin 2 columns (10 kDa molecular weight cutoff, Sartorius). The resulting ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated FBS (Sartorius, Göttingen, Niedersachsen, Germany), penicillin 100 U/mL ...
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bioRxiv - Systems Biology 2023Quote: ... filtered through a 10 kDa MWCO spin filter (Vivaspin500; Sartorius), and quantified via Nanodrop OneC at A205 nm.
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bioRxiv - Microbiology 2020Quote: ... then 100 mL of the appropriately diluted sample was filtered through a cellulose nitrate membrane filter with a pore size of 0.45 μm (Sartorius, Goettingen, Germany) using a vacuum pump ...
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bioRxiv - Plant Biology 2020Quote: ... overlain by a 0.8 mm thick diffusive gel disc and a protective membrane disc (polyethersulfone, 0.45 μm pore size, 0.15 mm thick, Sartorius Stedim, Göttingen, DE), encased by a plastic sampler housing (see Zhang & Davison (1995 ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated with a Vivaspin 20 centrifugal concentrator with a 30,000 Da molecular weight cut-off polyethersulfone membrane (Vivaspin 30k concentrator) (Sartorius, Göttingen, Germany) and injected onto a HiLoad Superdex 75 prep grade size-exclusion column (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... CM ultrafiltration and concentration was carried out by tangential flow filtration (TFF) using a Vivaflow 50 R 10,000 MWCO Hydrosart membrane (Sartorius, Göttingen, Germany) and a Masterflex L/S Easy Load machine (Antylia Scientific ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were concentrated using a Vivaflow® 200 crossflow cassette with a 10000 MWCO PES membrane (Sartorius, Göttingen, Germany; Cat.# VF20P0) and loaded onto a 5 mL GE Healthcare HisTrap™ FF Crude prepacked column (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... adding 3 ml of ice-cold Tris buffer and by filtrating the PLs through 0.45 μm pore-size membrane filters (Sartorius Stedim Biotech). Filters were washed twice with 3 ml of Tris buffer and allowed to dry ...
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bioRxiv - Cell Biology 2023Quote: ... The formulations were then diafiltrated against a 10 × volume of PBS (pH 7.4) through a tangential-flow filtration membrane with a 100 kDa molecular weight cut-off (Sartorius Stedim Biotech), concentrated to the desired concentrations ...
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). The purified and concentrated protein was delivered to GeneScript-Nanjing to raise the anti-rabbit polyclonal antibody.
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bioRxiv - Synthetic Biology 2020Quote: ... filtered through a 10 kDa MWCO spin filter (Vivaspin 2; Sartorius), and quantified by Nanodrop OneC ...
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bioRxiv - Immunology 2021Quote: ... concentrated 10-fold using 100,000 MWCO Vivaspin centrifugal concentrators (Sartorius, Germany) and used for neutralisation assays ...
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). Concentrations of the purified proteins were measured using BCA protein assay kit (Beyotime ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using a Vivaspin Turbo Ultrafiltration unit (10 kDa MWCO; Sartorius) and buffer-exchanged in 10 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... biotinylated RBD (10 μg/mL) was immobilised on streptavidin biosensors (Sartorius) for 300 s ...
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bioRxiv - Microbiology 2024Quote: ... Pooled fractions were concentrated using 10 kDa cutoff centrifugal filters (Sartorius) and dialyzed overnight in storage buffer (20mMTris–HCl pH8.0 ...