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Citations for Sartorius :
1 - 50 of 409 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... A CIMac pDNA 0.3 mL weak anion-exchange analytical column (Sartorius, Germany) was used with a Shimadzu
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bioRxiv - Synthetic Biology 2022Quote: ... pure Q-GRFT was prepared by flow-through membrane chromatography on a salt-tolerant anion exchange capsule (Sartobind STIC PA nano, Sartorius, Goettingen, Germany) according to manufacturer instructions.
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bioRxiv - Bioengineering 2021Quote: ... Virus was partially purified using depth filtration and anion exchange chromatography with syringes and small-scale filters (C0SP 23 cm2 [Merck] and Sartobind Q Nano [Sartorius]). Buffer compositions and other parameters were as previously described but scaled proportionately to the product and filter sizes (5) ...
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bioRxiv - Bioengineering 2024Quote: ... then filter sterilized via 0.2 µm membrane (Sartorius) filtration ...
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bioRxiv - Microbiology 2024Quote: ... filtered through a 5 µm membrane (Sartorius AG, Göttingen, Germany) to discard oocysts and empty shells ...
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bioRxiv - Microbiology 2024Quote: ... filtered through a 5 µm membrane (Sartorius AG, Göttingen, Germany) to discard oocysts and empty shells ...
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bioRxiv - Molecular Biology 2023Quote: ... The media was then filtered through a 0.45 µm filter (STARLAB, Hamburg, Germany) and concentrated using a 10 KDa Vivaflow tangential flow filtration (TFF) membrane (Sartorius, Göttingen, Germany) to a volume of 10-15 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and centrifugation (10 min, 6,000 x g) the supernatant was membrane filtered (Minisart RC 15, 0.45 µm, Sartorius AG, Göttingen, Germany) and used for analysis ...
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bioRxiv - Microbiology 2024Quote: ... Microbial biomass was collected from 10 litres of water filtered through five 47 mm diameter 0.2–µm polyethersulfone membrane filters (Type 15407, Sartorius Stedim Biotech) using a sterile glass filter holder (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 kDa MWCO PES membranes (Sartorius); supplemented by NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Microbiology 2023Quote: ... the virus-containing medium was filtered through a 0.45 µm membrane filter (Sartorius®) after 2.5 days ...
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bioRxiv - Molecular Biology 2022Quote: ... Buffer exchange was accomplished using Vivaspin concentrators (Sartorius). Protein purity was assessed by SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Microbiology 2024Quote: ... and buffer exchange to PBS using VivaSpin columns (Sartorius) was carried out ...
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bioRxiv - Microbiology 2023Quote: ... and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Neuroscience 2023Quote: ... The solution (pH 7.0-7.5) was sterile filtered with a hydrophobic PTFE membrane filter (pore size: 0.2 µm, Sartorius) before injection via a sterile 50 or 60 mm length 31 gauge sharp-tip steel cannula (Plastics One) ...
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bioRxiv - Bioengineering 2021Quote: Analyses were performed using MODDE version 13 (Sartorius). The main analysis combined the two experiments using a dummy variable denoting each experiment (EXP) ...
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bioRxiv - Genomics 2022Quote: ... diluted to OD600 = 0.5 and mixed in a 1:1 ratio and spotted onto 0.22 µm nitrocellulose membrane (Sartorius) placed on top of LBA (20 µL) ...
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bioRxiv - Cell Biology 2024Quote: ... pollen contained in 5 ml suspension was vacuum-filtrated onto a round polyamide membrane filter (diameter 5 cm, pore size 0.45 µm; Sartorius) and transferred onto 3.5 ml solid (0.25 % phytagel [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2023Quote: ... Equal quantities of both bacteria were mixed and spotted onto a cellulose acetate membrane filter (0.2 µm, 25 mm, Huberlab, Sartorius) placed on BHI agar with 500 µM DAP ...
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bioRxiv - Biochemistry 2020Quote: ... Imidazole was removed by buffer exchange using a Vivaspin centrifugal filter (Sartorius) with a 10 kDa cut-off ...
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bioRxiv - Microbiology 2024Quote: ... samples were clarified using a syringe with a filter cascade (5 µm, 1.2 µm, 0.8 µm, 0.45 µM, Sartorius AG). The cell pellets were not further analysed ...
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bioRxiv - Systems Biology 2023Quote: ... Cell dry weight was determined by vacuum filtration of 1 mL of samples on pre-weighed 0.45 µm filter membranes (Sartorius Biolab), followed by 15 min microwaving at 325 W and placement in a desiccator for at least 3 days.
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
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bioRxiv - Microbiology 2022Quote: ... concentrated on a Vivaflow 200 concentration system with a 10 kDa-cutoff membrane (Sartorius). The pH of the concentrated supernatant was adjusted to 8.0 with 100 mM Tris HCl and avidin was added to a final concentration of 1 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: Vivacon 500 columns with a 10 kDA Molecular weight cutoff membrane (Sartorius, Göttingen, Germany) were equilibrated with lysis buffer and centrifuged (14,000× g ...
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bioRxiv - Microbiology 2023Quote: ... After two buffer exchanges with trypsin buffer on Vivaspin 15R (5 kDa) columns (Sartorius), the samples were digested by trypsin with 1/80 (w/w ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was sliced off using a hot flame-sterilised scalpel and a sterile 25 mm diameter 0.2-µm membrane (Sartorius GmbH, Goettingen, Germany) was fixed between the cap and the test tube ...
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bioRxiv - Biochemistry 2020Quote: ... followed by concentrating and buffer exchange into IMAC binding buffer using Vivaflow 100 system (Sartorius). The resulting protein solution was purified by Histrap column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... collected from each sampling point in the mesocosm experiment was filtered through 0.2 µM pore cellulose acetate membrane filter (Sartorius Stedim Biotech, Göttingen, Germany). Nucleic acid was subsequently extracted from each of the filtered samples using the PowerWater DNA Isolation Kit (MoBio ...
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bioRxiv - Microbiology 2023Quote: ... 30 ml of seawater was sampled using a 50 ml syringe and subsequently filtered through a 0.45 µm filter (Sartorius Minisart, cellulose acetate membrane). Discarding the initial 10 ml of filtrate ...
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bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were centrifuged at 2000 rpm for 10 minutes and passed through a 0.45 µm syringe filter (Sartorius) to remove debris prior to concentration by ultracentrifugation at 25000 rpm for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting sample was subject to buffer exchange in 30 kDa MWCO Vivaspin Turbo centrifugal filters (Sartorius) against 25 mM HEPES/NaOH ...
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bioRxiv - Microbiology 2024Quote: ... the 2-MEA was removed by washing and buffer exchange to PBS using 100kDa Vivaspin6 columns (Sartorius), and left overnight to enable successful reoxidation of the disulfide bonds between antibody chains.
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bioRxiv - Molecular Biology 2021Quote: ... to 0.1 M Glycine / 10 mM Tris / pH 7.5 using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA ...
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bioRxiv - Molecular Biology 2021Quote: ... injection grade (≥ 10 volume changes) using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Buffer exchanged DS preparations were further adjusted to a concentration of ∼1 mg x mL-1 in 20 mM Tris / 150 mM NaCl / pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Microbiology 2024Quote: ... the tubes were centrifuged at 4600x g for 10 minutes and the supernatants were double sterile filtered with 0.2 µm pore size Minisart Syringe Filter (Sartorius, Germany), before storage in aliquots at −80 °C until use.
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 8000x g for 10 minutes and sterile filtered twice with 0.2 µm pore size Minisart Syringe Filter (Sartorius, Germany). This process was repeated after an additional incubation with the A ...
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bioRxiv - Immunology 2021Quote: ... The TFF retentate was next enriched for AAV particles on a cation exchange chromatography column (BIA Separations, Sartorius). The eluate was concentrated ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2022Quote: ... Buffer exchange and concentration was done with Vivaspin 20 ultrafiltration unit having a 1.000.000 molecular weight cut-off (Sartorius). Viral particles were washed and resuspended in PBS and titered using quantitative PCR with primers targeting WPRE element.
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated toxins were purified using buffer exchange columns (Vivacon 500, Sartorius, 3000 Da Molecular Weight Cut-Off) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... performed by ultrafiltration through a 10 kDa cut-off polyether sulfone membrane (Vivacell 250, Sartorius Stedim Biotech GmbH, Germany). The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the data were statistically analyzed using MODDE 13 Pro software (Umetrics, Sartorius, Malmö, Sweden). Four variables were established as independent factors to be investigated ...
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bioRxiv - Neuroscience 2024Quote: Purified mouse aSyn was concentrated to 5 mg/mL (347 µM) using a 10 kDa molecular weight cutoff (MWCO) spin filter (Sartorius, Columbus, OH) operated at 4,500 × g ...
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bioRxiv - Neuroscience 2024Quote: Purified human A53T aSyn or mouse aSyn was concentrated to 5 mg/mL (347 µM) using a 10 kDa molecular weight cutoff (MWCO) spin filter (Sartorius, Columbus, OH) operated at 4,500 × g ...