Labshake search
Citations for Sartorius :
101 - 138 of 138 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... two-fold serial dilutions of the biotinylated avi-tagged CH505 TF chSOSIPv4.1 and Man5GlcNAc2-enriched CH505 M5.G458Y chSOSIPv4.1 Env trimers were immobilized on hydrated Streptavidin (SA) Tips (Octet® Sartorius) for 120 seconds ...
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bioRxiv - Bioengineering 2023Quote: ... to CD123 WT ECD and variants (analytes in solution) was performed using Streptavidin capture biosensor (SA) (Sartorius, PN: 18-5019). CaptureSelect™ Biotin Anti-LC-kappa (Murine ...
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bioRxiv - Bioengineering 2023Quote: ... IL-2Rα and IL-2Rβ were immobilized to streptavidin-coated tips for analysis on an Octet Red96 BLI instrument (Sartorius). Less than 5 signal units (nm ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μM of small-molecule targets with biotin conjugated were used as ligands to be immobilized to the Octet Streptavidin biosensors (Sartorius). Titration experiments were performed in 25C ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Neuroscience 2023Quote: ... Octet® SAX high precision streptavidin biosensors and Octet® 384TW tilted-bottom plates were purchased from Sartorius (Göttingen, Germany). Peptides were synthesized by GenScript (Piscataway ...
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bioRxiv - Microbiology 2024Quote: ... 5 µg/mL anti-ALFA tag biotinylated nanobody in BLI buffer was immobilized on the surface of streptavidin-coated biosensors (Sartorius) to produce a total response of 1-2 nm ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspC (3 µg/mL) in PBS containing 2% w/v BSA (buffer) was captured onto Octet SA (streptavidin) biosensors (Sartorius) for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA duplex was captured at a concentration of 1.25 µg/ml in BLI Buffer (ELB containing 0.05% Tween-20 and 5 mg/ml BSA) on an Octet® Streptavidin (SA) Biosensor (Sartorius). His-tagged X ...
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bioRxiv - Biochemistry 2024Quote: ... A 20-kDa fragment of HA with a single biotin molecule per chain (Creative PEGWorks, 250 nM) was immobilized onto streptavidin tips (Sartorius). These tips were then incubated with purified ACAN proteins at a series of concentrations for 180 s during the association phase ...
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bioRxiv - Biochemistry 2024Quote: ... was carried out using GST-PHRF1RP and GST-PHRF1RP(P221L) and biotinylated unmodified nucleosomes (25 nM) immobilized on streptavidin biosensors (Sartorius). All samples were prepared in 20 mM MOPS (pH 7.0) ...
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bioRxiv - Bioengineering 2023Quote: ... and homogenized using a bead tissue homogenizer (Mikro-Dismembrator U, Sartorius) (n=4-6 per group) ...
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bioRxiv - Bioengineering 2024Quote: ... and homogenized using a bead tissue homogenizer (Mikro-Dismembrator U, Sartorius) (N=6 per group) ...
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bioRxiv - Biochemistry 2021Quote: A stock solution of sfGFP-ACE2 was diluted to at 10 μg/ml for immobilization onto High Precision Streptavidin (SAX) biosensors (Sartorius, Germany) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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bioRxiv - Biochemistry 2022Quote: ... A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1) was prepared by ligation and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG) at 50 nM concentration for 7 min at 500 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... The 35-mer template with a biotin-TEG at the 5’-overhang was annealed to an 11-mer primer (Supplementary Table 1) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). A chimeric RNA-DNA primer demonstrating the highest affinity to Polα (45 ...
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bioRxiv - Microbiology 2024Quote: ... 500 µl glass beads (diameter 0.10–0.11 mm, Sartorius AG, Göttingen, Germany) were added to 1 mL sample and cells disrupted with a FastPrep®24 homogenizer (M ...
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bioRxiv - Microbiology 2023Quote: ... cells were mechanically lysed by bead beating (Mikro-Dismembrator S from Sartorius) as described previously ...
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bioRxiv - Developmental Biology 2024Quote: ... The bead solution was filtered by a 0.22 µm syringe filter (Sartorius) into 500 µL of 500 mM MES buffer (pH 6.0) ...
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bioRxiv - Microbiology 2021Quote: ... A 5 μg/ml concentration of biotinylated stable O1/Manisa/TUR/69 FMD VLP (61) (kindly provided by Alison Burman) was immobilised on streptavidin-coated biosensors (Sartorius UK Limited) for 900 s ...
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bioRxiv - Biochemistry 2022Quote: ... In our BLI experiments, ACE2 Fc-fusions are tethered to either Streptavidin biosensors (WT-ACE2, CVD293) (Sartorius®, Item no.: 18-5019) or ProtA biosensors (Sartorius® ...
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bioRxiv - Biochemistry 2024Quote: Binding kinetics of ZHER2-CK2α were measured by biolayer interferometry (BLI) on an OctetRed384 using in-house made HER2-Fc-AviTag immobilized on streptavidin biosensors (Sartorius, 18-5021). Kinetic measurements were performed in PBS with 0.1% bovine serum albumin and 0.05% Tween 20.
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bioRxiv - Immunology 2022Quote: Biotinylated OspCA (3 μg/mL) in buffer (PBS containing 2% w/v BSA) was captured onto streptavidin biosensors (#18-5019, Sartorius, Goettingen, Germany) for 5 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The biotinylated SYK-tSH2 (50 μg/mL, C-terminal AviTag, N-terminal his tag cleaved) was loaded onto Octet Super Streptavidin Sensors (Sartorius 18-5057) in 50 μL 1XBLI kinetic buffer (Octet Kinetics Buffer 10 X ...
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bioRxiv - Immunology 2022Quote: ... GAGs had biotin attached at their reducing end with a previously described approach (35) before immobilisation to High Precision Streptavidin (SAX) biosensors (Sartorius AG, Goettingen, Germany). To achieve this SAX biosensors were hydrated for 10 mins in assay buffer (10 mM Hepes ...
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bioRxiv - Immunology 2022Quote: ... GAGs were biotinylated at their reducing end using an approach described elsewhere (44) and immobilised onto High Precision Streptavidin (SAX) biosensors (Sartorius AG, Goettingen, Germany). To immobilise ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads detecting cytokines from human were analyzed on an IQue Screener Plus (Sartorius). All assays were analyzed using quintuplicate determinations.
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bioRxiv - Cell Biology 2023Quote: ... Beads were separated using clarifying filters (2000 x g, 2 minutes; Vivaclear Mini, Sartorius).
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bioRxiv - Molecular Biology 2024Quote: ... Beads were removed from eluates using Vivaclear 0.8 µm PES centrifugal microfilters (Sartorius, VK01P042) and eluates were concentrated via Vivaspin 500 centrifugal concentrators (Merck ...
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bioRxiv - Immunology 2024Quote: ... beads were resuspended in FACS buffer and acquired on flow cytometer iQue®3 (Sartorius). Population gating and analysis of fluorescence intensity were performed with iQue Forecyt software ...
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bioRxiv - Immunology 2024Quote: ... beads were resuspended in FACS buffer and acquired on flow cytometer iQue®3 (Sartorius). Population gating and analysis of fluorescence intensity were performed with iQue Forecyt software ...
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bioRxiv - Cell Biology 2021Quote: ... to remove any residual beads and subsequently concentrated on pre-washed 100kDa cut off filters (Sartorius). Sample volume were equalized to 50 uL (5% of the samples were used as pulldown control for Westen blots analysis ...
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bioRxiv - Microbiology 2022Quote: ... Cells were disrupted by beat beating for 2 minutes using 0.4-0.6 mm glass beads (Sartorius). Beads were pelleted by centrifugation (21,000 × g ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... Fragmented gDNA was obtained by beat-beating the intact gDNA for 12 minutes using 2 mm diameter glass beads in a Mikro-Dismembrator U (Sartorius) at 2000 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... The wells were washed again with PBST-BSA and the beads resuspended in 90 µl with PBST-BSA and analyzed on an iQue screener (Sartorius). Signals reported are the median fluorescence intensities.
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bioRxiv - Microbiology 2020Quote: ... 1% (w/v) polyvinylpyrrolidone and 2% (w/v) sodium dodecyl sulfate after which 200 mg glass beads (0.11 mm Sartorius, Gottingen, Germany) were added and the cells were lysed for 5 min at 2000 rpm in a FastPrep VR-96 instrument (MP Biomedicals ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...