Labshake search
Citations for Sartorius :
51 - 100 of 536 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
-
bioRxiv - Neuroscience 2024Quote: ... 2% Ultroser G (Pall-Sartorius) and 2% Penicillin-Streptomycin (Life technologies) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
-
bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 2-3 mg/ml using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The final sample was concentrated to 20 mg/ml with a Vivaspin 2 device (2 kDa cutoff, Sartorius).
-
bioRxiv - Microbiology 2023Quote: ... 2 mM DTT) using a 5-KD concentrator (Sartorius). The purified samples were aliquoted ...
-
bioRxiv - Molecular Biology 2022Quote: ... were mixed together as well as eluates from samples (Pho92-HTP x 2 reps + Pho92-HTP ime4Δ/Δ x 2 reps) before concentration with Vivacon® 500 filtration cartridges (10 kDa MWCO; Sartorius, Cat# VN01H02) to a final volume of 120 μL ...
-
bioRxiv - Biophysics 2021Quote: ... using Vivaspin 2 columns (10 kDa molecular weight cutoff, Sartorius). The resulting ...
-
bioRxiv - Synthetic Biology 2020Quote: ... filtered through a 10 kDa MWCO spin filter (Vivaspin 2; Sartorius), and quantified by Nanodrop OneC ...
-
bioRxiv - Molecular Biology 2021Quote: ... depth filtered supernatants were sterile filtered through a Sartopore 2 (Sartorius) filter unit and stored until further processing at 2-8 °C.
-
bioRxiv - Physiology 2022Quote: ... 2 mM DTT) using a Vivaspin-20 concentrator (catalog no. VS2021, Sartorius). Five buffer exchanges with the storage buffer were performed and purified MBP was then aliquoted and stored at −80°C.
-
bioRxiv - Microbiology 2022Quote: ... and further concentrated to ∼100 µL using vivaspin 2 ultrafiltration units (Sartorius). All units had polyether sulphone filters with a molecular weight cut-off of 10 kDa ...
-
bioRxiv - Cancer Biology 2022Quote: ... Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
-
bioRxiv - Bioengineering 2019Quote: ... and disrupted with a micro-dismembrator (Sartorius, 2×30 s at 3,000 rpm). Subsequently ...
-
bioRxiv - Molecular Biology 2021Quote: ... injection grade and passed through a 0.45 + 0.2 µm Sartopore 2 300 (Sartorius) filter for sterile filtration ...
-
bioRxiv - Molecular Biology 2021Quote: ... Buffer exchanged supernatants were filtered using 0.8 + 0.45 µm Sartopore 2 filters (Sartorius) particulate contaminant removal and further sterile filtered using 0.2 µm Sartopore 2 filters (Sartorius) ...
-
bioRxiv - Neuroscience 2022Quote: ... then plates were imaged every 2 hours using an Incucyte Zoom system (Sartorius) to quantify the number of fluorescent cells per field of view.
-
bioRxiv - Cancer Biology 2022Quote: ... then imaged using the IncuCyte Live Cell Analysis System using the cell-by-cell module (Sartorius). For apoptosis assays ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were monitored in the Incucyte Live Cell Imager (Sartorius) for 24 h and analyzed for average cluster size over time ...
-
bioRxiv - Biochemistry 2022Quote: ... concentrated to 7.1 mg/ml with a 100-kDa MWCO Vivaspin 2 concentrator (Sartorius), frozen in liquid nitrogen ...
-
bioRxiv - Biochemistry 2021Quote: ... ultrafiltration was performed using Vivaspin 15 and Vivaspin 2 Hydrosart devices (Sartorius Stedim Biotech) of 2-kDa molecular-mass cutoff ...
-
bioRxiv - Systems Biology 2021Quote: ... A 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
-
bioRxiv - Molecular Biology 2021Quote: ... particulate contaminant removal and further sterile filtered using 0.2 µm Sartopore 2 filters (Sartorius), then divided into four parts for further processing ...
-
bioRxiv - Microbiology 2023Quote: ... filtered through a slow filter paper (#293; 1–2 µm Sartorius, Thermo Fisher Scientific), and then used to determine electrical conductivity (EC) ...
-
bioRxiv - Bioengineering 2023Quote: ... After sterile filtration through 0.45|0.2-micron PES Sartopore 2 filter (Sartorius Stedim, Germany) the formulated bulk sample was collected in 3 L Flexboy 2D bag (Sartorius Stedim ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads were separated using clarifying filters (2000 x g, 2 minutes; Vivaclear Mini, Sartorius).
-
bioRxiv - Cancer Biology 2021Quote: Cell death and cell confluence were determined using live-cell imaging in the IncuCyte Zoom and S3 (Sartorius). For cell confluence 50×103 cells were seeded in Matrigel-coated 6-well plates ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation assays and live-cell imaging were performed using the Incucyte SX5 Live-Cell Analysis System (Sartorius).
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to single-cell cloning on the CellCelector (Sartorius) into 96-well plates ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were imaged using an Incucyte Zoom Live Cell Imager (Sartorius) over time and analyzed using the IncuCyte Analysis Software (Sartorius) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Two peaks were collected and concentrated on a 2 mL Vivaspin 10 MWCO (Sartorius, UK). The molecular sizes of peak 1 (300 kDa ...
-
bioRxiv - Microbiology 2019Quote: ... 2 mM MgSO4) and concentration of the sample using Vivaspin6 5 kDa MWCO concentrators (Sartorius).
-
bioRxiv - Molecular Biology 2022Quote: ... 42 and 63 days were concentrated using 2 ml Vivaspin concentrator columns (Sartorius, Göttingen, Germany) by centrifuging at 1,000g until the final volume was 100 μl and subsequently centrifuged into the column cap at 4,000g for 2 min ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant from this second spin step was subsequently passed through 2 0.22µm filters (Sartorius) and made up to 500 ml with 50 mM ammonium acetate buffer at pH 5 before loading onto a 30 mL SP Sepharose column (Cytiva) ...
-
bioRxiv - Immunology 2024Quote: ... 10% FBS was added to the cells and real-time cell death analysis was performed using IncuCyte S3 Live-Cell Analysis instrument (Sartorius).
-
bioRxiv - Cancer Biology 2019Quote: ... the cell confluency was measured using an IncuCyte Live-Cell Analysis (Sartorius). Confluence growth inhibition was calculated as the relative confluency compared to untreated cells.
-
bioRxiv - Cancer Biology 2023Quote: ... cells were transferred to the Incucyte S3 Live-Cell Analysis System (Sartorius) where cell count and confluency was determined ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were grown in an IncuCyte Zoom live cell imager (Essen/Sartorius) with images recorded every 4 h for 6 d after addition of the drug ...
-
bioRxiv - Immunology 2023Quote: ... and cells were monitored with the IncuCyte live-cell imaging system (Sartorius) for up to 4 days ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then incubated in the Incucyte live cell analysis instrument (Sartorius) and green fluorescence and phase images were captured every 3 hours for 48 hours total ...
-
bioRxiv - Microbiology 2024Quote: ... cells were imaged in the Incucyte SX5 Live-Cell Analysis System (Sartorius). Cell confluence and the percentage of GFP-positive area were quantified automatically with the Incucyte Analysis software ...
-
bioRxiv - Microbiology 2024Quote: ... cells were imaged in the Incucyte SX5 Live-Cell Analysis System (Sartorius). Cell confluence and the percentage of GFP-positive area were quantified automatically with the Incucyte Analysis software ...
-
bioRxiv - Bioengineering 2021Quote: Bioreactor cultivation was performed as duplicates in 2 L Biostat B-DCU bioreactors (Sartorius AG, Germany) with a working volume of 1.5 L ...
-
bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
-
bioRxiv - Microbiology 2023Quote: ... 2 L or 5 L scale (Biostat B-plus and B-DCU units, Sartorius Stedium Biotech) as previously described [PMID ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: EPO concentrated solution was stock in sterile and endotoxin-free 2 D bag (Sartorius Stedim, Germany) and all batches of EPO were pre-filled in SyriQ BioPure® 1mL syringe (Schott ...
-
bioRxiv - Immunology 2023Quote: ... The GRAd32 S-2P vector was expanded in a 2 L Bioreactor (Biostat B DCU; Sartorius). The titer of virus contained in the bulk cell lysates was quantified by qPCR ...
-
bioRxiv - Biochemistry 2023Quote: ... 2 mM DTT and concentrated to 200 μl with a 30-kDa cutoff concentrator (Sartorius, Germany). The protein concentration was determined via Bradford assay ...
-
bioRxiv - Cancer Biology 2020Quote: ... For analysis of cell death with the Incucyte live-cell analysis system (Sartorius), Yoyo3 iodide viability die was used at 1/8000 final concentration and images were acquired ...
-
bioRxiv - Immunology 2022Quote: Cell death was analyzed using an IncuCyte Live Cell Imager (Sartorius, Goettingen, Germany) at the Genome Engineering and iPSC Center at Washington University ...