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Citations for Sartorius :
51 - 100 of 207 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were concentrated using 30 kDa cut-off Vivaspin columns (Sartorius) and their concentration was determined by their absorbance at 280 nm.
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bioRxiv - Microbiology 2019Quote: ... Protein was concentrated to ~ 5ml using Vivaspin concentrator spin columns (Sartorius) at 4500 × ɡ ...
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bioRxiv - Cell Biology 2019Quote: ... the protein was concentrated after labelling using a Vivaspin concentrator (Sartorius), ultra-centrifuged (280.000 x g ...
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bioRxiv - Microbiology 2019Quote: ... Protein was concentrated to ~ 5ml using Vivaspin concentrator spin columns (Sartorius) at 4500 × ɡ ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was concentrated to 32 mg/ml using centrifugal concentrators (Sartorius) with a relative molecular mass cut-off of 100K.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was concentrated using a Vivaspin 500 column (Sartorius, Goettingen, Germany) column following the manufacturer’s instructions.
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... filtered using a 0.45 µm low protein retention syringe filter (Sartorius) and 0.5 mL aliquots were stored at −80 °C.
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... All proteins were diluted in Octet kinetics buffer (Sartorius, Göttingen, Germany). Antibodies were captured on anti-human Fc AHC2 tips (Sartorius) ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated 10x using Vivaspin™ protein concentrator spin columns (Sartorius, VS15T02) and stored at 4°C.
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bioRxiv - Immunology 2021Quote: Biolayer interferometry kinetic assays were performed in triplicate on the Octet® Red384 system (Sartorius) with a buffer containing PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Wound healing assays were performed using the Incucyte Live-Cell Analysis System (Incucyte Zoom, Sartorius). Cells were plated in 96-well plates (Essen Bioscience ...
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bioRxiv - Bioengineering 2020Quote: Biolayer interferometry (BLI) experiments for ACE2 competition assays were performed with an Octet RED96e (Sartorius). For ACE2 competition assays ...
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bioRxiv - Immunology 2023Quote: ... BLI ligand titration and binding kinetics assays were performed on an Octet Red96e system (Sartorius) at 30°C with an orbital shake speed of 1000 rpm ...
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bioRxiv - Biophysics 2024Quote: ... Assay was performed in seven sequential steps with Octet® BLI Discovery 12.2.2.20 software (Sartorius): Step 1 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was concentrated using a Vivaspin centrifugal concentrator (MWCO 10 kDa, Sartorius) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated with Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva) ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius) and washed each thrice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were concentrated using a Vivaspin6 10 kDa filter unit (Sartorius). The protein concentration was measured via absorption at 280 nm using a Nanodrop spectrophotometer (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: Competition binding assays were performed by a premix method with streptavidin bio-sensor (SA sensor, Sartorius). Firstly ...
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bioRxiv - Molecular Biology 2022Quote: ... migration- and apoptosis assays were performed and analyzed by Incucyte® Live Cell Analysis System (Sartorius Stedim Plastics GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... Fragmented DNA was concentrated using Vivaspin 20 columns (Sartorius; Cat #VS2032), and unspecific binding was blocked by incubating the sample with 1% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius, Göttingen, Germany), analyzed by SDS-PAGE and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... followed by centrifugation of denatured proteins on a filter device (Sartorius, Vivacon 500). After several washing steps with 8 M urea and ammonium bicarbonate ...
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bioRxiv - Biochemistry 2021Quote: ... The immobilization of ligand proteins on amine reactive second-generation (AR2G) biosensors (Sartorius) was done following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted to 100 nM in Kinetics buffer (Pall FortéBio/Sartorius). Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 30 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further used.
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 50 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further use ...
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bioRxiv - Synthetic Biology 2024Quote: ... and aqueous solutions containing protein components were filtered using Minisart syringe filters (Sartorius). 0.5x1mm (I.DxO.D. ...
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bioRxiv - Cell Biology 2022Quote: Scratch assay were performed and cell migration was monitored by the automated Incucyte ZOOM (Sartorius, Gottingen, Germany). 67NR cells infected with the FN mutant plasmids were seeded in an Essen Imagelock 96-well plate at the density of 1×60^4 cells/well and were maintained over night to reach 100% confluency ...
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bioRxiv - Immunology 2020Quote: Antigenicity assays were performed and analyzed using BLI on an Octet Red 96 System (Pall Forté Bio/Sartorius) at ambient temperature with shaking at 1000 rpm ...
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bioRxiv - Cancer Biology 2022Quote: Trans-well invasion assay was conducted using Incucyte® S3 Live-Cell Analysis Instrument (Sartorius, Bohemia, NY, USA) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD32a (FcγRIIA) and CD16a (FcγRIIIA) was measured in a biolayer interferometry assay on an Octet RED96 system (Sartorius). Streptavidin-coated biosensors were loaded with purified biotinylated human CD64 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation assays and live-cell imaging were performed using the Incucyte SX5 Live-Cell Analysis System (Sartorius).
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bioRxiv - Immunology 2024Quote: Real-time cell-death assays were performed using a two-color IncuCyte S3 Live-Cell Analysis instrument (Sartorius). Cell lines were seeded in 12 well plates and treated with cell death stimulating agents or infected with viruses ...
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bioRxiv - Biochemistry 2020Quote: Digestion of 10His-SUMO1T95R proteins was performed on 10 kDa-cutoff spin filters (Sartorius) according to a published protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was simultaneously concentrated and buffer-exchanged (with a Vivaspin Turbo 4; Sartorius) into PM buffer (80 mM Pipes pH 6.8 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were filtered using Minisart 0.45 μm filter (Sartorius Stedim Biotech, Goettingen, Germany). Part of the extract was set aside prior to immunoprecipitation and used for SDS-PAGE as described above ...
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bioRxiv - Bioengineering 2023Quote: ... The AFC-eluates were buffer exchanged with 10kDa Vivaspin protein concentrator (Sartorius Stedim, Germany) using Sorval Lynx XTR centrifuge (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Biophysics 2023Quote: ... Proteins solutions were concentrated to at least 600 µM with Vivaspin centrifugal concentrators (Sartorius) and stored at -80 °C.
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and Ulla proteins were concentrated to 35 ∼ 50 mg/ml using a VIVASPIN20 (Sartorius) concentrator and stored at −80 °C until use.
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 was immobilized on protein G biosensors (Sartorius Cat. No. 18-5082) at 5 µg/mL (in binding buffer ...
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bioRxiv - Genomics 2020Quote: ... DNA extracts were concentrated to 250 μl using Vivaspin® Turbo 15 (Sartorius) concentrators and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit ...
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bioRxiv - Biophysics 2020Quote: ... 0.32μM DNA) and loaded on centrifugal concentrators (Vivaspin 500; 10,000 MWCO PES, Sartorius). The quenched samples were concentrated to 60 μl by centrifugation at 20,000xg for 2 min (Sigma 1-16K) ...
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bioRxiv - Cancer Biology 2021Quote: Scratch wound migration and matrigel invasion assays were conducted with an IncuCyte S3 live-cell imaging system (Sartorius Bioscience). DU145 cells (4.5 × 105 ...