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Citations for Sartorius :
1 - 50 of 207 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... All interactions studies were performed with Streptavidin biosensors (Sartorius, Göttingen, Germany) conjugated to the biotinylate DNA substrates ...
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Immunology 2023Quote: Affinity measurements for the BTN2A1-BTN3A1 interaction were determined by BLI on an Octet Red 96e instrument (Sartorius). Assays were performed in black 96 well flat-bottom plates at 25°C with agitation (1000 rpm) ...
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bioRxiv - Immunology 2021Quote: All label-free interaction analyses were performed on an Octet HTX biolayer interferometry-based detection system (ForteBio/Sartorius, Gottingen, Germany) equipped with various sensor types ...
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bioRxiv - Immunology 2022Quote: The on- and off-rate and the equilibrium dissociation constant for the CR2 interaction with C3dg was measured using bio-layer interferometry (Octet, Sartorius). We loaded a his-tagged human CR2 protein (Bio-techne ...
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bioRxiv - Immunology 2022Quote: The Octet quantification assay for protein expression level was performed on an Octet 96 Red system (Sartorius). Harvested cell media were centrifuged and cell supernatants were prepared in a 96-well plate with S-2P or S-6P standards diluted in media from 20 μg/mL to 0.3125 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Cancer Biology 2021Quote: Tube formation assay of endothelial cells was performed using IncuCyte Angiogenesis 96-well PrimeKit Assay (Sartorius) with modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... the FcRn binding assay was measured in a biolayer interferometry assay on an Octet RED96 system (Sartorius). Streptavidin-coated biosensors were loaded with purified biotinylated human FcRn in pH 6.0 assay buffer (100mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Caspase-3/7 green apoptosis assay reagent (Sartorius #4440) was added to the cells and transferred to Incucyte® ZOOM 2FLR system and analyzed using 2016 an integrated software.
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bioRxiv - Molecular Biology 2022Quote: ... IncuCyte® Caspase-3/7 Apoptosis Assay Reagent (Sartorius Stedim Plastics GmbH ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Microbiology 2019Quote: ... Residual proteins were then eliminated by filtration on 0.22-µm high-protein-retention Ministart syringe filters (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2023Quote: ... Assays were performed in triplicate on an Octet Red384 (Sartorius). Dissociation constants for 16AA-WT and 16AA-M6 interactions with Kdo2-lipidA were estimated by plotting response values at equilibrium as a function of concentration and fit to a global specific binding with the Hill slope model in Prism 9.
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bioRxiv - Biochemistry 2023Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30℃ with shaking (1000 rpm) ...
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bioRxiv - Cell Biology 2023Quote: Cell death assays were performed using Incucyte ZOOM from Sartorius. Cell death was measured by Sytox Green inclusion (Thermo Fisher Scientific #S7020) ...
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bioRxiv - Biochemistry 2024Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30°C with shaking (1000 rpm) ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: Binding assays were carried out on an Octet RED96 instrument (Sartorius) using a previously established protocol (He et al. ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated using a Vivaspin concentrator (Sartorius) with a half lower MWCO than the size of the protein being purified ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Immunology 2022Quote: ... Assays were acquired using an IncucyteTM S3 live-cell analysis system (Sartorius). Each well was imaged in 15 min intervals for 24–30 h with the image lock module and the 20× objective ...
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bioRxiv - Cancer Biology 2024Quote: ... and plates were placed into an Incucyte Live-Cell Assay incubator (Sartorius) for 72 hours ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated using centrifugal concentrators (VivaSpin, Sartorius) and then assessed for successful labeling by measuring fluorescence using the following settings and the monochromator in the BioTek Synergy Neo2 plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...
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bioRxiv - Cancer Biology 2021Quote: Chemotaxis assays were performed in the IncuCyte S3 imager as recommended by Sartorius; additional experimental details are provided in the Supplemental Methods ...
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bioRxiv - Biochemistry 2024Quote: The epitope binning assay was conducted using the Octet RED 96 system (Sartorius). Anti-IFNγ antibody (ab180547 ...
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bioRxiv - Biochemistry 2021Quote: ... All protein samples were concentrated using Vivaspin centrifugal concentrators (Sartorius). Denaturing gel electrophoresis was performed using NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Then proteins were transferred into ultrafiltration tubes (10kDa, Sartorius AG) and washed three times with 50 mM NH4HCO3 at 14000g for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then concentrated (Vivaspin 30,000 MWCO, Sartorius) and ultracentrifuged in a TLA-120.2 rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Bioengineering 2024Quote: ... the refolded protein was concentrated using a VIVAFLOW50 system (Sartorius). The concentrated sample was filtrated with 0.22 µm membrane filter ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, cat #4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius ...
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bioRxiv - Neuroscience 2022Quote: Chemokinesis of iMGLs was analyzed using IncuCyte® scratch wound cell migration assay (Sartorius) with real-time visualization according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: An epitope competition assay was performed via BLI on an Octet RH96 platform (Sartorius) using an in-tandem assay format ...
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bioRxiv - Cancer Biology 2023Quote: Killing assays were performed using an Incucyte S3 Live-Cell Analysis System (Sartorius, Germany). nEGFP-labeled target cells were seeded in flat-bottom ...
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bioRxiv - Genetics 2023Quote: A monolayer scratch assay was performed on a 96-well Essen ImageLock plate (Sartorius) using the IncuCyte® WoundMaker Tool (Sartorius ...
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bioRxiv - Biophysics 2024Quote: ... Assay was performed in three sequential steps with Octet BLI Discovery 12.2.2.20 software (Sartorius): Step 1 ...