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Citations for Sartorius :
51 - 100 of 597 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 5 mM CaCl2 pH 8.0 (3 times the original volume) using a 10 kDa molecular weight cut-off centrifugal filter (Vivaspin® Turbo 15; Sartorius, UK), before flash-freezing in liquid nitrogen and storage at −80°C until required.
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bioRxiv - Biochemistry 2023Quote: ... Fractions that contained BLMcore and no detectable nuclease activity were concentrated in Vivaspin-20 10 kDa concentrator (Sartorius) to 2 mL and loaded onto the HiPrep 16/60 Sephacryl S-300 HR column (Cytiva ...
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bioRxiv - Biophysics 2022Quote: BLI analyses were performed at 25°C at an acquisition rate of 5 Hz averaged by 20 using an Octet© BLItz System (FortéBio/Sartorius) with assays were designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing FKBP12 F36V were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of FKBP12 F36V was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The supernatant (EV-depleted HES) was removed and concentrated using a VivaSpin 20 centrifugal concentrator with a 5 kDa molecular weight cutoff (Sartorius). Purified EVs were washed twice with PBS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA duplex was captured at a concentration of 1.25 µg/ml in BLI Buffer (ELB containing 0.05% Tween-20 and 5 mg/ml BSA) on an Octet® Streptavidin (SA) Biosensor (Sartorius). His-tagged X ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Bioengineering 2022Quote: ... the appropriate volume of stock solution was centrifuged at 3000g at 10 °C in Vivaspin® 20 concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2022Quote: ... the combined clarified fluid was directly concentrated to 10-50 fold by Vivaspin 20 Centrifugal Concentrators (Sartorius, Gottingen, Germany) with PES membrane of 100k MW cut off ...
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bioRxiv - Microbiology 2023Quote: ... This monodisperse peak was pooled and concentrated to 20 mg/ml with a 10 kDa MWCO ultrafiltration device (Sartorius).
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
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bioRxiv - Microbiology 2024Quote: ... each filter was conditioned for 48h at a constant temperature of 20°C ± 1°C and 50% ± 5% relative humidity values in a controlled environment (SCC 400L Climatic Cabinet, Sartorius, Varedo (MB), Italy) ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Microbiology 2023Quote: ... at 24 hpi the Incucyte S3 live cell imaging system (Sartorius) was utilised to measure eGFP fluorescence ...
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bioRxiv - Neuroscience 2023Quote: Mouse microglia and hiMGL conditioned media were digested using filter aided sample preparation (FASP) as previously described.22 For microglia lysates Vivacon spin filters (Sartorius) with 30 kDa cut-off were used ...
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bioRxiv - Neuroscience 2024Quote: ... using Vivaspin Turbo 15 100,000 MWCO (#VS15T41; Sartorius). The genome titer of rAAV was determined by Pico Green fluorometric reagent (#P7589 ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Microbiology 2024Quote: ... levels against A/American Wigeon/South Carolina/22/000345-001/2021 (A(H5N1)) were measured using BioLayer Interferometry (BLI) on an Octet RH96 instrument (Sartorius AG, Germany). Assays were set up on Greiner Bio 96-well black plates and AR2G biosensors (Sartorius AG ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... Thirty micrograms of protein resulting from each fraction was subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol 20 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethylphosphine) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 6.0) using a centrifugal concentrator (Vivaspin Turbo 15, Sartorius) and concentrated to a final concentration of 1 mg/mL ...
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bioRxiv - Microbiology 2020Quote: ... every 5 min for up to 20 h or until a quasi-equilibrium was reached using a micro-balance (Sartorius MSE3.6P-000-DM, readability 0.0010 mg). The chamber of the micro-balance was half-opened to keep air circulating with the environmental chamber ...
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bioRxiv - Plant Biology 2021Quote: ... the pH of the hydroponic culture media of the 5 treatments was measured using a pH meter (PB-10, Sartorius, Germany).
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Cancer Biology 2023Quote: Cells were plated in 24 well plates and images were obtained using Incucyte (Sartorius) using whole well imaging at 4X ...
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Pathway metabolite ratios reveal distinctive glutamine metabolism in a subset of proliferating cellsbioRxiv - Cancer Biology 2024Quote: ... and media replenished at 24 hours for cell confluency measured using IncuCyte-SX5 (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: ... 24 hours after seeding a scratch wound was made using the WoundMaker Tool (Sartorius) and the cells were incubated in low-serum medium after washing twice ...
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bioRxiv - Cell Biology 2023Quote: ... All cells tested negative for Mycoplasma contamination in periodic PCR tests (Sartorius, 20-700-20).
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bioRxiv - Biochemistry 2024Quote: ... 50 KDa MWCO Vivaspin 20 filtration units and Vivaspin 20 pressure heads were purchased from Sartorius. Nickel nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Neuroscience 2024Quote: Purified human A53T aSyn or mouse aSyn was concentrated to 5 mg/mL (347 µM) using a 10 kDa molecular weight cutoff (MWCO) spin filter (Sartorius, Columbus, OH) operated at 4,500 × g ...
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bioRxiv - Neuroscience 2024Quote: Purified mouse aSyn was concentrated to 5 mg/mL (347 µM) using a 10 kDa molecular weight cutoff (MWCO) spin filter (Sartorius, Columbus, OH) operated at 4,500 × g ...
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bioRxiv - Biophysics 2021Quote: ... concentrated using Vivaspin Turbo 15 30-kDa centrifugal filter units (Sartorius), and stored at 4°C until use ...
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bioRxiv - Microbiology 2020Quote: ... The purified Rel was concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 50 kDa) ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Microbiology 2023Quote: ... part of 0.22 μm filtrate was filtered through tangential flow filtration equipment (TFF) with a 100 kDa tangential flow membrane package (Sartorius, VIVAFLOW 50 100,000 MWCO, Germany) to obtain virus-free production water ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated using VIVASPIN 20 Columns (Sartorius), flash frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated using VIVASPIN 20 Columns (Sartorius), flash frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using VIVASPIN 20 Columns (Sartorius), flash frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were imaged every 24 hours on the Incucyte S3 Live-cell analysis system (Sartorius) using brightfield and 10x objective and capturing 16 fields per well ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue culture plate was incubated for 24 hours before transferring into an IncuCyte S3 (Sartorius) with the same incubation conditions ...