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Citations for Sartorius :
1 - 50 of 597 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The biotinylated MOW-15-22 RBD was diluted to 10µg/mL in 10× Octet kinetics buffer (Sartorius) and loaded onto hydrated Streptavidin biosensors to 1nm shift ...
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bioRxiv - Developmental Biology 2024Quote: ... for a total of 15-20 minutes with trituration every 5 minutes using 200 μl low bind tips (Sartorius, 790101F). Papain was inactivated using an Ovomucoid inhibitor (Worthington Papain Dissociation Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 10-kDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to ∼15-20 mg/ml using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Microbiology 2023Quote: ... were dialyzed against 20 mM Tris-HCl (pH 8.0) and concentrated to 10-15 mg/ml using Vivaspin Ultra concentrators (Sartorius, Göttingen, Germany). Purity of the proteins was analyzed after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 30-KDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were imaged at 15 DIV for 24 hours using the IncuCyte S3 system (Essen Bioscience Sartorius Cat#4763). Using the IncuCyte S3 2019A software (Essen Bioscience Sartorius ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated in a Vivaspin 20 MWCO 10 filter (Sartorius) to 4.8 mg/mL ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.5) was used for gel filtration and Vivaspin 15 with 5 MWCO (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... appropriate fractions (selected by 4-20% gradient SDS-PAGE) were pooled and concentrated by centrifugal filtration (Vivaspin 20, 5,000 MWCO PES, Sartorius) for loading onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... Fusion events were visualized for 24 h at 20 min intervals using the IncuCyte® S3 Live-Cell Analysis System (Sartorius). Phase contrast and GFP images (4 per well ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0 was concentrated to 10 mg.mL-1 using 10 kDa molecular weight cut-off spin filter (Vivaspin® Turbo 15; Sartorius, UK) and screened using commercially available screens ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing BLMcore were concentrated in Vivaspin-20 10 kDa concentrator (Sartorius) to 2 mL and loaded onto HiPrep 16/60 Sephacryl S-300 HR column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Obtained EHEP was then concentrated to 15–25 mg/mL using Vivaspin-4 10K columns (Sartorius, Göttingen, Germany). About akuBGL ...
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bioRxiv - Bioengineering 2024Quote: ... WT and 0-F5 cells (n=4 per line) were cultured in the ambr®15 (Sartorius, Germany) system in CD CHO medium supplemented with 0.01% antibiotic-antimycotic and 8 mM glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... individual frames of cells growing under normal culture conditions were acquired every 5 minutes for 24 hours using an IncuCyte ZOOM live-cell analysis system (Sartorius) equipped with a 20 x objective.
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bioRxiv - Biophysics 2021Quote: ... 50 mM KCl) using Vivaspin 20 columns (10 kDa molecular weight cutoff, Sartorius). This step was followed by cation exchange chromatography using a Mono-S-HR 10/10 FPLC column pre-equilibrated with SAU-50 and SAU-1000 buffers ...
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bioRxiv - Biophysics 2021Quote: ... The eluate from this step was concentrated to 10-15 mL with a Vivaspin® concentrator (Sartorius). Concentrated sample was then exchanged into IEX W buffer (same as IMAC W ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were pooled and concentrated with Vivaspin 20 concentrators (10 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (Cytiva) ...
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bioRxiv - Immunology 2022Quote: ... concentrated via ultra-filtration using Vivaspin 20 centrifugal columns (10 kDa cut-off, Sartorius), and loaded on the SEC column (Superdex 200 26/ 60 ...
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bioRxiv - Biochemistry 2022Quote: ... appropriate fractions were pooled and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). In pHisSUMO-rnSTAT2 preparations ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius), and purified via size-exclusion chromatography with a Superdex S75-10/300 column (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 30 mg/ml using Vivaspin 20 concentrators (10 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... prior to concentration (approximately 10-fold) using a Vivaspin 20 MWCO 100kDa centrifugal concentrator (Sartorius) according to manufacturer’s recommendations.
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bioRxiv - Biochemistry 2024Quote: ... pooled and concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Vivaspin® Turbo 15; Sartorius, UK) prior to being applied to a S75 column pre-equilibrated in 25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... and appropriate fractions were pooled and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: ... Material for analyses was obtained from seeds pre-cooled for 15 min in 5-mL Teflon capsules (Sartorius GmbH, Göttingen, Germany), containing a 10 mm-diameter agate bead ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated ~5-fold using a 10 kDa Sartocon Slice ECO Hydrosart cassette (Sartorius), and diafiltered against 4 volumes of 10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... dispersed in deionized water with different pH were ultrasonically treated for 10 min at a frequency of 24 kHz (Sartorius labsonic M, Germany). The difference in the hydrodynamic diameter of the nanocomposites was investigated using a Zetasizer Nano-ZS-90 Dynamic Light Scattering (DLS ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the proteins were identified by SDS-PAGE and concentrated using a Vivaspin Turbo 15 centrifuge filter with a 10 kDa molecular weight cutoff (Sartorius). Glycerol was added to a final concentration of 25% ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Evolutionary Biology 2024Quote: ... Filtered HES was concentrated using a VivaSpin 20 centrifugal concentrator with a 5 kDa molecular weight cutoff (Sartorius). EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). A second SEC was performed on an Äkta prime plus FPLC (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-MC and ES5838 (4 x 104) cells per well were plated in a 24-well plate and placed in an IncucyteS3 incubator (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Nine images per well were taken every 6 hrs for seven days ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated by centrifugation at 4,000xg at 4°C through VivaSpin 10 kDa MWCO concentrators (Sartorius), aliquotted ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Plant Biology 2023Quote: ... and centrifugation (10 min, 6,000 x g) the supernatant was membrane filtered (Minisart RC 15, 0.45 µm, Sartorius AG, Göttingen, Germany) and used for analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... and concentrated 40x by passing through VIVASPIN 20 MWCO 10 kDa centrifugal concentrator columns (Sartorius Stedim Biotech), as per manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated by centrifugation (3000g for 20 min) using a 10 kDa molecular mass cutoff (MWCO) column (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Biophysics 2024Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...