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Citations for BioLegend :
151 - 200 of 268 citations for U Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Levels of murine cytokines in transplanted mice were assessed using the LEGENDplex™ Murine Inflammation panel (13-plex) in a V-bottom plate per manufacturer’s instructions (Cat. No: 740446, BioLegend, San Diego, CA, USA). LEGENDPlex data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... cells from the dermis and epidermis were combined and cultured overnight in the presence of 10 U/ml IL-2 (Biolegend). The following antibodies were used in flow analysis of skin associated γδ T cell populations ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Immunology 2020Quote: ... CD69) as well as the Cell Hashing Antibodies (HTOs) were purchased from BioLegend. PBMCs from different donors were independently stained with one of our HTO-conjugated antibody pools (based on the experimental condition ...
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bioRxiv - Cancer Biology 2022Quote: ... “Rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). Scratches were imaged at 0 hours ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with 100 μL/well TMB-substrate (Biolegend/ITK). The substrate reacted approximately 8 minutes ...
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bioRxiv - Immunology 2023Quote: ... Culture medium is supplemented with 1000U/well murine GM-CSF (Biolegend, Cat#576302) and a predetermined dose of P815-IL4 supernatant ...
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bioRxiv - Immunology 2023Quote: ... and PE/Dazzle™ 594 anti-human CD8 (301058, BioLegend; 1 μL/well). After washing with FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... and then incubated with CD28 Monoclonal Antibody (CD28.2) (302934, BioLegend; 0.5 μL/well), CD49d (Integrin alpha 4 ...
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bioRxiv - Genomics 2023Quote: ... Cells were then resuspended in 100 mL/well of FC-block (Biolegend, 422302) (1:100 in Perm buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as 7-AAD Viability Staining Solution (420404) (Biolegend, San Diego, CA). Lymphoid populations were characterized using APC anti-mouse CD3 (100236 ...
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bioRxiv - Immunology 2020Quote: ... in the presence or absence of human IFN-β (500 U/ml: Pestka Biomedical Laboratories) or IL-27 (100 ng/ml: BioLegend) without adding IL-2.
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bioRxiv - Immunology 2021Quote: ... Splenocytes were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Cancer Biology 2019Quote: ... and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend, CA ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS and blocked with 50 µl/well (Human FcX, BioLegend) for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The “rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). After 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... wells were stained with Zombie Viability Dye Aqua (1 μL/mL, BioLegend Cat. 423101) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... to count the absolute number per well by adding Precision Count Beads™ (BioLegend) to the samples ...
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bioRxiv - Cell Biology 2023Quote: μ-side 8-well Ibidi chambers were coated with an anti-CD31 antibody (102502, BioLegend) for 30 min ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... as well as PE-conjugated anti-CD25 (1:100, Clone M-A251, Biolegend, 356101). These cells were subsequently fixed and permeabilized with a Human FOXP3 buffer set (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Immunology 2020Quote: ... as well as 7-AAD and Zombie Red viability dies were all purchased from BioLegend. AH1-loaded ...
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bioRxiv - Immunology 2021Quote: ... The wells were incubated with biotinylated anti-mouse IFN-γ (clone R4-6A2, Biolegend, USA) for 16 hours at 4° C ...
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bioRxiv - Immunology 2021Quote: ... Cells were activated in a 24-well plate coated with anti-CD3 (clon: 500A2, Biolegend) and soluble anti-CD28 (clon ...
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bioRxiv - Immunology 2021Quote: ... Lung cells were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and 50 μl/well of TMB High Sensitivity substrate solution (BioLegend, UK) was added ...
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bioRxiv - Genomics 2023Quote: ... Control wells were created in the same manner by immobilising commercially biotinylated Mouse IgG2a (Biolegend) alone at a concentration of 0.5ug/ul ...
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bioRxiv - Immunology 2023Quote: ... 24-well plates have been coated with 1μg purified human TCRγ/δ antibody (331202, BioLegend) per well overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... as well as 0.5 ug each of the 10 TotalSeq-B oligo-tagged antibodies (BioLegend) listed in Supplementary Table 3 were added and cell pellets were suspended ...
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bioRxiv - Genomics 2024Quote: ... cells were split into two wells and cultured with either 10ng/mL rhIL-12 (Biolegend), 5ng/mL rhIL-2 ...
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bioRxiv - Cell Biology 2023Quote: ... quantification of PG release from cartilage explants was performed using the dimethylmethylene blue assay (36, 37) and exposure to sialidase (1.9 U/mL) and interleukin-1 beta (Cat #. 300-01A; BioLegend, San Diego, CA, USA) (10 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice using 150 µl/well intracellular-staining Perm-Wash buffer (BioLegend, cat. 421002) diluted 1:10 in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 100ng per well of recombinant Spike RBD protein in 1×coating buffer (Biolegend) overnight at 4C° ...
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bioRxiv - Pathology 2022Quote: ... was added to the wells and waited 10 minutes stopped by TMB stop solution (423001, Biolegend). Each well was analyzed for the optical density (OD ...
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bioRxiv - Immunology 2019Quote: ... 3 sections per well) with Alexa647-conjugated anti-EpCAM (1/160 dilution; clone G8.8, 118212, Biolegend), eFluor660-conjugated anti-E-cadherin (1/200 dilution ...
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bioRxiv - Immunology 2020Quote: ... wells were supplemented with 10 μg/ml of αLFA1 antibody (Clone: TS 1/18, BioLegend, 302116) or with 10 μg/ml of αFAS-L antibody (BD ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed and incubated with 3,3′,5,5′-Tetra-methyl benzidine substrate (Biolegend, San Diego, CA), followed by a stop solution (Biolegend) ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly in 100 μl/well of anti-mouse IFN-γ (clone XMG1.2, 1:200, Biolegend) plus anti-mouse TNF-α (clone MP6-XT22 ...
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bioRxiv - Immunology 2022Quote: ... as well as SARS-CoV-2-S1 (biotin-coupled; Acro; #S1N-C82E8) and fluorochrome-coupled streptavidin (Biolegend). The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH ...
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bioRxiv - Immunology 2019Quote: ... and Alexa Fluor-568 goat antirabbit secondary antibody as well as anti-perforin–Alexa Fluor 488 (BioLegend) and phalloidin–Alexa fluor 647 (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... each well used for culture was incubated with 3 μg/mL anti-CD3 (Biolegend clone 145-2C11) and 3 μg/mL anti-CD28 (Biolegend clone 37.51 ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as antibodies to cell surface markers (anti-45 AF700 [Biolegend, 147716], anti-CD3e FITC [Biolegend, 100306] ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were activated in six-well plates coated with anti-CD3e (OKT3; 317326; BioLegend) in presence of soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: 24 well plates were coated with anti-CD3/28 stimulating antibodies (Biolegend Catalog Nos. 100302 and 102102) at final concentration of 5 µg/mL each overnight at 4° C ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was aspirated and cells were resuspended in 25 µL FcR block per well (200x, Biolegend #101320) diluted in FACS buffer (2 % FBS ...
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bioRxiv - Neuroscience 2024Quote: ... wells were incubated overnight at 4°C with mouse anti-β-III-tubulin (1:500, Biolegend, #MMS435P). After washing with PBST ...