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Citations for BioLegend :
101 - 150 of 268 citations for U Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Cell Biology 2024Quote: ... were added to the isolated lymphocyte suspension before seeding at 1mL/well in a 24-well plates coated with 1µg/mL of anti-CD3ε (100340, Biolegend) 24 h before the procedure.
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Microbiology 2020Quote: ... and 50 ng/ml GM-CSF + 500 U/ml IL-4 (Biolegend, https://www.biolegend.com), respectively ...
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Immunology 2022Quote: ... blocked with 5 µL/well Human FcX (BioLegend) for 15 min ...
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bioRxiv - Bioengineering 2022Quote: ... wells were developed using TMB substrate (Biolegend, 421101) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... wells were developed using TMB substrate (Biolegend, 421101) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... as well as anti-B220-Pacific Blue (BioLegend).
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bioRxiv - Immunology 2022Quote: ... After 1hr cells in the bottom chamber (migrated) were analysed by flow cytometry with precision count beads (Biolegend).
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bioRxiv - Immunology 2020Quote: ... Cells were placed in V-bottom plates and stimulated with 500X Cell Activation Cocktail with Brefeldin A (Biolegend) in IMDM medium supplemented with 8% FCS ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then thawed and plated in 24-well plates (2-2.5 millions/1ml/well) pre-coated with anti-CD3 (2.5 ug/ml, HIT3a clone, Biolegend, San Diego, CA) for 24 hours before harvesting ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend) and 50 ng/ml rhGM-CSF (Biolegend ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells (1×105 cells/well) and Nalm6-GD2 (1×105 cells/well) were plated in Nalm6-GD2 culture medium containing 1×Brefeldin A (Biolegend, Cat# 420601) for 5-7h ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Microbiology 2020Quote: ... as well as human CD8 were purchased from Biolegend.
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bioRxiv - Immunology 2022Quote: ... as well as anti-Bw6-FITC (Biolegend, 1:40), for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... fixed with 100 μl/well of Fixation Buffer (Biolegend) for 20 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... APC anti-human CD3 (300412, BioLegend; 1 μL/well), and PE/Dazzle™ 594 anti-human CD8 (301058 ...
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bioRxiv - Immunology 2023Quote: ... PE anti-human CD27 (302808, BioLegend; 1 μL/well). After being stained ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Bioengineering 2021Quote: ... wells were incubated with anti-mouse HRP -IgG antibody (BioLegend). The plate was washed three times with washing solution (200 μl/well for 5 minute) ...
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bioRxiv - Biochemistry 2021Quote: ... wells were probed with HRP-streptavidin (Biolegend 405210, 1000:1) or biotinylated anti-a αvβ3 antibody (Biolegend 304412 ...
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bioRxiv - Cell Biology 2021Quote: ... as well as flotillin (anti-flotillin-1 antibody, 849802, BioLegend) as a DRM marker (Salzer and Prohaska ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... The wells were washed once with Annexin V buffer (Biolegend) and cells were stained with Annexin V FITC and 7AAD (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... and then for 15-30min with 100ul/well TMB (BioLegend) substrate solution ...
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bioRxiv - Cell Biology 2021Quote: ... as well as anti-mouse CD16/32 and CD127 (Biolegend). Cells were washed twice with phosphate-buffered saline (PBS)-bovine serum albumin (BSA).
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bioRxiv - Immunology 2023Quote: ... wells were pre-coated with anti-CD3ε (Biolegend, #145-2C11) prepared in PBS at 1 μg/mL for 2-3 hours at 37°C before being emptied for seeding ...
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bioRxiv - Genomics 2023Quote: ... directly add 100 mL/well of IL-1β (Biolegend, 508208) at 2X (1:50 in perm buffer ...
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bioRxiv - Immunology 2024Quote: ... The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel catalog #740493 with the V-bottom plate was used according to manufacturer’s instructions (BioLegend, CA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... 100 U/mL penicillin and 100 μg/mL streptomycin supplemented with 20 ng/mL M-CSF (BioLegend) at a density of 2×105 cells/mL ...
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bioRxiv - Immunology 2023Quote: ... 8-10 week old C57BL6/J mice received either 200ng of IFNα (Biolegend 752802, ∼ 1×104 U) or saline intranasally (N = 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Synthetic Biology 2020Quote: ... as well as poly-clonal chicken anti-Neu5GC antibody (Biolegend, USA) plus secondary staining with FITC-labelled donkey anti-chicken IgY (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... as well as True-Stain Monocyte Blocker (BioLegend, San Diego, CA) for the myeloid panel ...
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bioRxiv - Cell Biology 2022Quote: ... as well as FITC or APC conjugated Annexin V dye (Biolegend).
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bioRxiv - Immunology 2023Quote: ... PerCP/Cyanine 5.5 anti-human CD19 (302230, BioLegend; 1 μL/well), PE anti-human CD27 (302808 ...
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bioRxiv - Immunology 2023Quote: ... as well as 2 µL of Monensin (Biolegend, San Diego, CA). Cells were cultured for 6 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: Mice were injected intravenously under isoflurane anesthesia once daily with 1×105 U recombinant mouse IFNγ (575306, Biolegend) in 150 μL PBS ...
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bioRxiv - Immunology 2022Quote: Activated γδT cells were seeded in round-bottom plates with FITC conjugated anti-mouse CD107a (1D4B) antibody or anti-human CD107a (H4A3) (Biolegend, San Diego, CA). γδT cells were co-cultured with A20 or K562 at a ratio of 1:1 ...
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bioRxiv - Bioengineering 2023Quote: ... coated glass bottom chamber and then labeled with 1ugꞏml−1 of Alexa Flour (AF) 488 conjugated anti-human CD45 antibodies (Biolegend, Cat# 304017, clone HI30) in 2% BSA in PBS for 20 min at RT ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 50 µL/well of fixable Zombie Green viability dye (BioLegend) diluted 1/1000 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as antibodies to cell surface markers (anti-45 AF700 [Biolegend, 147716] ...
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bioRxiv - Immunology 2023Quote: ... wells were pre-coated with 5 μg/mL ICAM-1 (Biolegend, #553006) for 2-3 hours at 37°C before being emptied for seeding ...
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bioRxiv - Physiology 2023Quote: ... with or without EPO (35U/well) (recombinant mouse EPO carrier-free, Biolegend). The ratio of splenocytes to RBCs was 1:1 ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...