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Citations for BioLegend :
651 - 700 of 8186 citations for Mouse Stem Cell kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and then in a T cell activation analysis cocktail including: anti-mouse CD3 clone 17A2 APC conjugate (BioLegend, 100235), rat anti-mouse CD4 AlexaFluor700 conjugate (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were stained for VEGF-A using antibodies against mouse VEGF-A (cat# 512809, clone 2G11-2A05, Biolegend) and AlexaFluor647-conjugated secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... These fixed and permeabilized cells were further stained with mouse anti-human IFN-γ antibody conjugated with PE (BioLegend) and Brilliant Violet 421 conjugated mouse anti-human TNF antibody (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Cell viability was assessed using the Zombie NIR™ Fixable Viability Kit (Biolegend) as per the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Live/dead (LD) cells were identified with Zombie aqua fixable viability kit (Biolegend). CD3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested and stained with Live/Dead Aqua staining kit from BioLegend as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: The cells were stained with Zombie Green™ Fixable Viability Kit (BioLegend, USA) in PBS for 15 min at RT and in the dark using a dilution of 1:500 ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ or naive CD8+ T cell enrichment kit (Biolegend, San Diego, CA). Cell purity for all enrichments ranged between 90-98% ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were surface stained with the Zombie Aqua Fixable Viability Kit (Biolegend, #423101) for 0.5hr at RT,1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The green dye CFSE Cell Division Tracker Kit (BioLegend, San Diego, California, USA) and DAPI (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and stained with Zombie NIR™ Fixable Viability Kit (BioLegend) to label dead cells ...
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bioRxiv - Immunology 2020Quote: ... Dead cells were excluded using Zombie Aqua Fixable Viability Kit (BioLegend, Cat: 423102). For intracellular cytokine stainings ...
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bioRxiv - Immunology 2021Quote: ... Dead cells were first excluded using Zombie Aqua™ Fixable Viability Kit (BioLegend). Cells were then incubated with fluorescence conjugated antibodies in HBSS containing 2% BCS at 4℃ for 30 minutes to stain surface markers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with Zombie NIR™ Fixable Viability Kit (Biolegend, CA). Prior to acquisition ...
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bioRxiv - Immunology 2022Quote: ... The cells were then fixed with the True-Nuclear Transcription Factor kit (Biolegend). After fixation and permeabilization ...
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bioRxiv - Microbiology 2022Quote: ... and sCD40L were measured using the human B cell premix kit from Biolegend Legendplex™ using manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with either the FITC Annexin V Apoptosis Detection Kit (BioLegend) or CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were immediately stained with a Zombie NIR Viability kit (#423106, BioLegend, USA) diluted 1:5000 in PBS for 20 min in the dark at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with a Zombie NIR Fixable Viability kit (BioLegend, catalog #423105) for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell death was measured using the annexin V-APC Apoptosis Detection Kit (BioLegend) according to the manufacturer’s instructions and analysed with a BD Fortessa X20 (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... To exclude dead cells Zombie Aqua Fixable Viability Kit (#423102, 1:100, BioLegend) was added ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were purified by a negative selection Kit (BioLegend, Cat# 480035), and purified CD8+ T cells were activated by 1 μg/ml anti-CD3 (BioXCell ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were purified by a negative selection Kit (BioLegend, Cat# 480035). Purified CD8+ T cells were activated by 1 μg/ml anti-CD3 (BioXCell ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with Zombie NIR™ Fixable Viability Kit (Biolegend, Cat# 423105) for 30 min at 4 ℃ before cell surface staining ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2023Quote: NK cells were isolated from frozen PBMCs by negative selection using the MojoSort™ Human NK Cell Isolation Kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet™ fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11 ...
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Bioengineering 2022Quote: ... HRP-conjugated anti-mouse secondary HRP goat anti-mouse IgG antibody (#4053, Biolegend, USA) (1:5000 in TBST ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... the following biotinylated anti-mouse or anti-mouse/human antibodies were purchased from BioLegend: CD4 (clone RM4-5) ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human/mouse Granzyme B conjugated to PerCP/Cyanine5.5 (clone QA16A02, Biolegend, 372211), mouse anti-human Perforin conjugated to PE/Cyanine7 (clone B-D48 ...
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bioRxiv - Immunology 2021Quote: ... the cell suspensions were stained with the following antibodies: PE anti-mouse Ly-6C (BioLegend, 128008, clone HK1.4, 1:200), PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Immunology 2021Quote: Cytokine levels in supernatants from stimulated cells were determined using the LEGENDplex™ Mouse anti-viral response panel (Biolegend, USA) as per manufacturer’s instructions and read on BD Accuri™ C6 Plus Flow Cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Immunology 2019Quote: Splenic TFH and TFR cell were stained with antibodies against mouse CD4 (Clone: RM4-5, Biolegend, San Diego, CA, USA), CD44 (Clone:IM7 ...
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bioRxiv - Cancer Biology 2020Quote: ... This was followed by a 10 min incubation with anti-mouse Fc block and a 30 minute cell surface staining with anti-mouse CD4-APC/Cy7 and CD8-PE/Cy7 (Both from Biolegend). CFSE labeled CD8+ CTLs were subsequently sorted on a BD FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were incubated with APC (allophycocyanin) or PE (phycoerythrin)-conjugated anti-mouse Ly-6A/E (Sca-1) antibody (#122508, BioLegend), APC or PE-conjugated anti-mouse CD45 antibody (#103106 ...
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bioRxiv - Immunology 2020Quote: ... Antibody dilutions in cell staining buffer containing 1 % TruStain fcX™ (anti-mouse CD16/32) Antibody (both BioLegend, London, UK) were used to stain according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...