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Citations for BioLegend :
601 - 650 of 8186 citations for Mouse Stem Cell kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: For differentiation of live and dead cells we stained cells with the Zombie Violet Fixable Viability Kit according to the manufacturer’s instructions (BioLegend). For surface marker analysis ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Evolutionary Biology 2021Quote: ... Lcn-2 levels were determined in fecal supernatants using a LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit (BioLegend #443708).
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bioRxiv - Microbiology 2020Quote: Levels of cytokines and soluble mediators present in plasma of infected mice were quantified using LEGENDplex™ Mouse Inflammatory Panel kit (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: OVA-specific IgE levels were measured using a LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit (BioLegend, San Diego, CA). The plate was washed 4 times with wash buffer followed by addition of Matrix A to standard wells and Assay Buffer to sample wells ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD107b (Mac-3) and anti-mouse TNF (MP6-XT22) (all Biolegend). Live-Dead Fixable Green and Live-Dead Fixable Aqua Dead Cell stains was purchased from Thermofisher (Boston ...
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bioRxiv - Microbiology 2021Quote: ... APC mouse anti-mouse CD64 (Clone X54-5/7.1, Biolegend, San Diego, CA), APC-Cy7 rat anti-mouse CD11b (Clone M1/70 ...
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bioRxiv - Immunology 2021Quote: Indicated mouse cytokines were measured using the LEGENDplex Mouse Macrophage/Microglia Panel (BioLegend). Samples were prepared according to manufacturer's instructions and analysed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... anti-mouse CD3 (145-2C11) and anti-mouse CD28 (37.51) were from BioLegend. Anti-human CD3 (OKT3 ...
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bioRxiv - Immunology 2021Quote: ... rat anti-mouse IL-6 or rat anti-mouse Ly6G (all from Biolegend), followed by fluorescence-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... rat purified anti-mouse CD49d antibody (For VLA-4α, BioLegend 103701, 1µg/mouse), rat IgG2b κ control antibody (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Rat purified anti-mouse CD11a antibody (For LFA-1α, BioLegend 101101, 1µg/mouse), rat purified anti-mouse CD49d antibody (For VLA-4α ...
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bioRxiv - Bioengineering 2024Quote: ... FITC anti-mouse CD8a antibody and APC/Cyanine7 anti-mouse CD4 antibody (BioLegend) in PBS ...
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bioRxiv - Physiology 2024Quote: ... mouse anti-mouse CD64-PE/Dazzle594 (clone X54-5/7.1, cat# 139320, BioLegend), rat anti-mouse PDGFRα-PE/Cy5 (clone APA5 ...
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bioRxiv - Immunology 2021Quote: ... the block was washed off and the cells were stained with 50μl of Anti-Mouse anti-CD-11b-BV785 (clone M1/70, Biolegend) and anti-F4/80-BV650 (clone BM8 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 1 h on ice in PE/Cy7 anti-mouse CD45.2 antibody (clone:104, BioLegend) and PE-conjugated anti-mouse IL1a antibody (clone:ALF161 ...
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bioRxiv - Microbiology 2022Quote: ... The followed anti-mouse antibody panels were used for cell subset analysis: CD45 (clone 30-F11, Pe-Cy7, Biolegend), CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... Hosts were culled at 4 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... 400 μl cell suspension were incubated with 80 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... cells were cultured overnight with or without 4 μg/mL Syrian hamster anti-mouse CD28 (clone 37.51, BioLegend, USA) and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Neuroscience 2020Quote: ... cortical cells from embryonic mice were labelled with Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2) antibody (BioLegend, #140327). Cells were incubated with antibody at 4 °C for 20 minutes followed by washing with wash buffer (Ca2+/Mg2+ free PBS ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 8 or 12 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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bioRxiv - Immunology 2022Quote: ... The suspension of mouse CD4+ T cells and the spinal cord sections were stained with APC-Cy7-conjugated anti-mouse CD4 (clone GK1.5, Biolegend), BV510-conjugated anti-mouse CD4 (clone RM4-5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc receptors were blocked by incubating cells with TruStain FcX™ PLUS anti-mouse CD16/32 antibody (Biolegend, USA) for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: Single cell suspensions isolated from treated tumors are individually barcoded with hashtag antibodies (Anti-mouse TotalSeq-C antibodies, Biolegend). Each sample was washed 4 times with PBS + 2% BSA before pooling ...
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bioRxiv - Immunology 2019Quote: 106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat ...
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bioRxiv - Immunology 2019Quote: ... dissociated GL261 tumors (5 × 106 cells) were pre-incubated with 1.0 μg of TruStain fcX (anti-mouse CD16/32, clone 93; BioLegend) in 100 μL of FACs Buffer (DPBS with 1% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were collected by scraping and stained with APC-conjugated anti-mouse H-2Kb bound to SIINFEKL (141606, BioLegend), PerCP-conjugated anti-mouse CD86 (105025 ...
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bioRxiv - Cell Biology 2021Quote: ... Then, cells were staining with CD8 (mouse CD8-APC-Cy7, eBioscience, 47-0081-82) and Zombie UV (Biolegend, 427107), followed by flow cytometry analysis.
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend). CD14+ monocytes were stimulated for 24 hours with 1ug/ml of LPS ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control, APC Mouse IgG2a, κ; #400220, Biolegend) or Anti-human/mouse CD49f antibody (#313620 ...
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bioRxiv - Cell Biology 2023Quote: ... the cell pellet was resuspended in 1% BSA/ PBS containing secondary fluorescence antibody (PE anti-mouse IgG1, BioLegend, 406608) and incubated for 1 h as described above ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with Alexa Fluor® 700 anti-mouse CD206 (MMR) antibody (1:200; Biolegend, C068C2) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of mouse cell-surface molecules: ① Purchased from Biolegend(USA): Anti-CD45-BV510(clone 30-F11);Anti-Ly-6G/Ly-6c(Gr-1)-PerCP(clone RB6-8C5);Anti-CD4-BV421(clone GK1.5);Anti-F4/80-FITC(clone BM8);Anti-CD8a-PE(clone 53-6.7) ...
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bioRxiv - Immunology 2023Quote: ... and around 1×106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... congenic CD45.1 mouse spleen was isolated and red blood cells were lysed with RBC lysis buffer (BioLegend, CA, USA). Leukocytes were then resuspended in complete RPMI with 1µg/mL high molecular weight Poly(I:C) ...
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bioRxiv - Immunology 2023Quote: ... Primary mouse lung fibroblasts were freshly isolated after magnetic bead-based negative selection of epithelial cells (118204, Biotin anti-mouse CD326 Epcam, Clone: G8.8, BioLegend), endothelial cells (102404 ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Molecular Biology 2023Quote: ... cells were stained with the following monoclonal antibodies to determine cellular architecture of most abundant spleen and immune cells (Table S10): anti-mouse CD45.2 (Biolegend), anti-mouse Gr-1 (eBioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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bioRxiv - Immunology 2024Quote: ... These fixed and permeabilized cells were further stained with mouse anti-human IFN-γ antibody conjugated with PE (BioLegend) and Brilliant Violet 421 conjugated mouse anti-human TNF antibody (BioLegend) ...