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Citations for BioLegend :
151 - 200 of 3514 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies binding markers: CD81 (cat# 104902, Biolegend 1:1000), and ALIX (cat# 634502 ...
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bioRxiv - Immunology 2024Quote: ... FcR binding sites were blocked by using αCD16/CD32 (BioLegend) and cell viability was assessed by staining with Live/Dead Fixable Aqua Staining (Life technologies) ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with Annexin V Binding Buffer (Biolegend, 422201) and stained for 15 min at RT with annexin V (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... CD46 APC (targets MCP protein) clone TLA-2-10 (cat# 352405, BioLegend®), CD49d APC (targets α4 integrins ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used in each sample were: mouse anti-HA 1:2000 (Biolegend, 901513; HEK293), rabbit anti Cav2.3 N-terminus 1:250 (Covalab ...
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bioRxiv - Immunology 2023Quote: Human: Anti-human CD141 (clone M80, BioLegend), anti-human CD45RA (clone HI100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified anti-His tag mouse antibody was from BioLegend (USA). Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... incubating the cells with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 2 μl of anti-human CD3-AF488 (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE (clone 61D3 ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were stained for 30 minutes with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 Spike RBD protein was mixed with Streptavidin-BV421 (405225, BioLegend) at a 4:1 molar ratio for 1 hour at 4 °C to obtain the antigen probe ...
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Immunology 2020Quote: ... blocked for Fc binding (Clone 2.4G2 for mice or TruStain (BioLegend) for human) ...
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bioRxiv - Immunology 2022Quote: ... Binding of biotinylated antibodies was detected using fluorochrome-conjugated streptavidin (Biolegend). Flow cytometry analyses were performed on a Fortessa flow cytometer (BD ...
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bioRxiv - Immunology 2020Quote: ... then RDB binding cells were recognized by APC/Cy7 Streptavidin (Biolegend). The antibodies used included anti-B220-PE (RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... non-specific Fc binding was blocked with TruStain FcX PLUS (BioLegend) for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with Annexin V Binding Buffer (Biolegend, 422201) and stained with APC Annexin V (Biolegend ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with an antibody that recognizes the luminal epitope of the human LAMP1 protein (clone H4A3, BioLegend) conjugated with Alexa fluor 488 or 647 for 30 minutes ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Wells were washed and incubated with 3,3′,5,5′-Tetra-methyl benzidine substrate (Biolegend, San Diego, CA), followed by a stop solution (Biolegend) ...
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bioRxiv - Cancer Biology 2019Quote: ... and resuspended in 100 µL of Annexin V Binding Buffer (BioLegend, 422201) with allophycocyanin (APC)-conjugated Annexin V (1:20 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Annexin V binding buffer (Biolegend, Cat #422201) instead ...
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bioRxiv - Immunology 2022Quote: ... The binding complexes were detected by HRP goat anti-rat IgG (BioLegend) and TMB substrate.
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bioRxiv - Cancer Biology 2022Quote: ... was added to cells resuspended in 200 uL of binding buffer (BioLegend). Cells were then incubated for 30 minutes at room temperature in the dark before centrifuging them again ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to cells resuspended in 200 uL of binding buffer (BioLegend). Cells were then incubated for 30 minutes at room temperature in the dark before centrifuging them again ...
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bioRxiv - Cancer Biology 2022Quote: ... in combination with the antibody cocktail (above) in Annexin binding buffer (BioLegend) at 1:50 dilution in combination with DAPI as live/dead cell stain.
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in Annexin V binding buffer with Annexin V BV421 (Biolegend; 640924) and incubated for 15 minutes at room temperature protected from the light ...
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bioRxiv - Bioengineering 2023Quote: ... then resuspended in Annexin V Binding Buffer (BioLegend, San Diego, CA, USA) and incubated at room temperature in the dark with FITC-Annexin V (1:20 antibody to binding buffer by volume ...
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bioRxiv - Physiology 2023Quote: ... and non-specific binding was blocked with TruStain FcX Plus (Biolegend; 1µg). Staining for cell surface markers was performed using fluorophore-conjugated primary antibodies for 30 minutes at 4×C ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells stably expressing EPO-eGFP and A1At-mCherry were incubated with 1ug/mL Brefeldin A (Biolegend) for 12 hr ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend). Phenotyping was performed on a NovoCyte Advanteon flow cytometer and FACS was performed on a BD LSRII.
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...