Labshake search
Citations for BioLegend :
51 - 100 of 3514 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
-
bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
-
bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
-
bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
-
bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
-
bioRxiv - Bioengineering 2021Quote: ... and APC anti-human CD46 (clone TRA-2-10, Biolegend 352405). Antibody staining was performed for 20 minutes on ice ...
-
bioRxiv - Immunology 2022Quote: ... APC-anti human 2B4/CD244 (2-69; BioLegend, San Diego, CA); or with the isotype control APC mouse IgG1 (MOPC-21 ...
-
bioRxiv - Immunology 2019Quote: ... Antibodies include mouse anti-human ataxin-3 65042 1H9-2 (BioLegend), mouse anti-human MIC60 ab110329 (Abcam) ...
-
bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
-
bioRxiv - Immunology 2022Quote: ... 1:50 diluted anti-human CD32-APC (BioLegend, clone FUN-2), and 1:50 diluted anti-human CD64-PE/DAZZLE594 (BioLegend ...
-
bioRxiv - Immunology 2023Quote: ... and 1 U/mL of recombinant human IL-2 (BioLegend, #589102) in 200 μL of complete culture medium (see above for media components) ...
-
bioRxiv - Bioengineering 2023Quote: ... and 500 IU/ml of recombinant human IL-2 (Biolegend, 589104). Stimulated T cells were co-transduced via spinoculation at 931xg ...
-
bioRxiv - Immunology 2023Quote: ... and 2 μl PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532). Additionally ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
-
bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
-
bioRxiv - Bioengineering 2021Quote: ... The His tagged ACE2 variants were detected using anti-His mAb (Cat. No. 652505, BioLegend).
-
bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
-
bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
-
bioRxiv - Microbiology 2021Quote: ... in Annexin V binding buffer (BioLegend) containing 50 U/mL hirudin for 20 min (RT ...
-
bioRxiv - Cancer Biology 2022Quote: ... in Annexin V binding buffer (Biolegend) for 30 minutes at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... in Annexin V binding buffer (BioLegend) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Annexin V binding buffer (BioLegend, 422201) and Propidium Iodide (Invitrogen ...
-
bioRxiv - Bioengineering 2023Quote: ... in Annexin V binding buffer (Biolegend) for 15 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
-
bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
-
bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
-
bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
-
bioRxiv - Immunology 2021Quote: ... Anti-human CD206 APC (1:50, clone 15-2, BioLegend, cat # 321110), Anti-human CD4 Alexa700 (1:50 ...
-
bioRxiv - Immunology 2021Quote: ... PE-anti-human-Nectin-2 (TX31) (all from BioLegend, San Diego, CA). The following antibodies were used for in vivo blocking experiments ...
-
bioRxiv - Immunology 2022Quote: ... 1-2×106 cells were incubated with Human TruStain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406 ...
-
bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
-
bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
-
bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
-
bioRxiv - Neuroscience 2024Quote: ... CD11b+CD45.2+CX3CR1+ microglia were sorted into a pre-cooled 5ml low-protein binding falcon tube (fisher scientific; Cat#: 13-864-407) containing 0.5ml cell staining buffer (BioLegend, Cat#: 420201). Walls of the tubes were washed and cells pelleted at 300g ...
-
bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
-
bioRxiv - Cell Biology 2023Quote: ... rinsed in AnnexinV Binding Buffer (BioLegend # 422201), and around 100000 cells were resuspended in 50µL Annexin V Binding Buffer ...
-
bioRxiv - Cancer Biology 2024Quote: ... and Annexin-V Binding buffer (Biolegend #422201) incubated with 7-AAD (Tonbo Bioscience #13-6993-T500 ...
-
bioRxiv - Cancer Biology 2024Quote: ... in Annexin binding buffer (BioLegend, Cat#422201). Cells were washed twice with flow staining buffer and fixed with 4% PFA for 10 min and resuspended in a final volume of 100 μL for flow cytometry analysis using the Cytek Aurora spectral flow cytometer (Cytek ...
-
bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
-
bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
-
bioRxiv - Immunology 2022Quote: ... 2 μl PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532, clone: MHM-88), 2 μl PE/Cyanine7 anti-human IgD antibody (BioLegend ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant human SARS-CoV-2 spike S1 IgG lyophilized antibody (Clone AM009105, BioLegend) was used as a standard.
-
bioRxiv - Immunology 2024Quote: ... washed twice HBSS containing 2% FCS and incubated with Human Trustain Fcx (Biolegend) for 20 min on ice ...
-
bioRxiv - Immunology 2022Quote: ... and APC anti-His Tag antibody (Biolegend) for 30 min each ...
-
bioRxiv - Immunology 2019Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ...
-
bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...