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Citations for BioLegend :
101 - 150 of 1133 citations for Medium Kit without Serum and with CultureBoost R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... and 40ng/ml IL-18) for day 1 intracellular cytokine staining (IL-12 from R&D, IL-7 and IL-15 from Biolegend).
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... PE anti-human TRAILR4 (DcR2) (R&DSystems, Clone 104918) or PE Mouse IgG1 κ Isotype Control (Biolegend, Clone MOPC-21) for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 10 ng/mouse of rmIL-12 (R&D) or 100 ng/mouse of rmIL-33 (Biolegend) alone or in combination in 50 µL directly in the tumor area twice a week from day 2 to day 26 ...
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bioRxiv - Immunology 2021Quote: ... IgG3 and IgA serum levels were determined using the LEGENDplex mouse immunoglobulin isotyping panel (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Serum cytokine analysis was performed using LEGENDPlex Mouse Inflammation Panel from BioLegend (Cat. No. 740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: For the detection of serum cytokines either the LEGENDplex Th Cytokine Panel™ from BioLegend or the BD CBA Mouse Inflammation Kit™ were used according to the manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... 20 µl/ml mouse serum and 10 µl/ml Fc block (CD16/32) (Biolegend, CA). Plates were incubated for 2 hrs at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were blocked using 5% goat serum and stained using antibodies against CD41 (133902, Biolegend) and laminin (L9393 ...
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bioRxiv - Immunology 2023Quote: ... in the culture medium containing 1×Brefeldin A (Biolegend, Cat#420601) in a 96-well plate for 5-7h ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in medium or in 1x MoJo buffer (Biolegend, UK).
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2024Quote: ... the medium was supplemented with 20 ng/ml IFN-γ (BioLegend, 575302) and 200 ng/ml LPS (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101; TNFα ELISA kit, BioLegend # 430204) or using LEGENDplex™ Human CD8/NK Panel Kit (Cat# 741186) ...
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Receptor surface expression levels were verified by flow cytometry using CXCR4- and CXCR7-specific mAbs (12G5, R&D Systems and 8F11-M16, BioLegend) on a BD FACS Fortessa cytometer (BD Biosciences).
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies diluted in blocking solution: 5% Normal Goat Serum (Biolegend, 927502), or 0.2% (w/v ...
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bioRxiv - Immunology 2021Quote: ... TNFa and IL6 were measured in serum samples using the LEGENDplex™ bead-based immunoassays from Biolegend per manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: Serum cytokine levels were assessed using the LEGENDplex™ Mouse Inflammation Panel (BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with antibodies diluted in a blocking solution of 5% Normal Goat Serum (BioLegend) in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6) (BioLegend, CA) and FITC anti-human KRAS antibody (Catalog# ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Immunology 2023Quote: ... serum samples were diluted 2-fold with assay buffer and standards were mixed with matrix solution (Biolegend) to account for additional components in the serum samples ...
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bioRxiv - Immunology 2022Quote: ... Heparinized blood was separated using Lymphopure density gradient medium (Biolegend, San Diego, CA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... this medium was supplemented with GM-CSF (40 ng/mL, Biolegend Catalog # 575906), and IL-13 (80 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... Culture medium is supplemented with 1000U/well murine GM-CSF (Biolegend, Cat#576302) and a predetermined dose of P815-IL4 supernatant ...
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bioRxiv - Cancer Biology 2021Quote: ... c-Kit (BioLegend), EpCam (G8.8 ...
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bioRxiv - Immunology 2019Quote: ... 1% bovine serum albumin and 0.3% Triton X-100) then stained with anti-gp100 antibody (clone HMB45, BioLegend) used at 1:30 followed by an anti-IgG2a secondary conjugated to Alexa555 (Invitrogen ...
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bioRxiv - Systems Biology 2019Quote: ... Stained cells were washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES and stained with streptavidin-PerCP/Cy5.5 (BioLegend). Stained cells were washed ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES) and stained with streptavidin-PerCP/Cy5.5 (BioLegend).
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bioRxiv - Immunology 2020Quote: ... Human trachea was stained for 72 hours in PBS serum and the following antibodies: hCD103-AlexaFluor 594 (Biolegend) 1:40 ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained in FACS buffer (PBS containing 0.5% bovine serum albumin and 0.05% sodium azide) with fluorescently labelled antibodies from BioLegend (CD11c-APC/Cy7 ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Immunology 2019Quote: Mice were intranasally dosed with 3 µg in 20 µl per mouse recombinant murine TNF-α (r-TNF-α, carrier-free, 575204, BioLegend Ltd, UK) every day for 3 days ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101 ...
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bioRxiv - Immunology 2022Quote: Serum samples were screened for neutralization using LEGENDplex SARS-CoV-2 neutralizing antibody assay (BioLegend, San Diego, CA, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2019Quote: The serum immunoglobulin levels were measured with LEGENDplex Mouse Immunoglobulin Isotyping Panel according to the manufacturer’s protocol (Biolegend, 740493).
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bioRxiv - Neuroscience 2022Quote: ... Muscle sections were incubated overnight at 4 °C in blocking solution (2 % BSA, 10 % Normal Goat Serum, 0.1 % Triton and PBS) with chicken anti-NFM (#822701, BioLegend, USA ...
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bioRxiv - Microbiology 2022Quote: Serum cytokine levels were determined using a beads-based immunoassay with a custom-designed mouse cytokine panel (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: The serum IgE levels of the mice were measured using Mouse IgE ELISA MAX™ Deluxe (BioLegend cat. 432404) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: IL-1β release was measured in murine serum using the LEGENDplex™ mouse inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as described above except shaking was performed at 800 rpm.
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bioRxiv - Immunology 2020Quote: ... followed by 10-minute incubation on ice with blocking buffer containing 10% mouse serum with 0.25 μg/ml anti CD16/CD32 (BioLegend). Cells where incubated with indicated antibodies (Supplementary Table 5 ...
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bioRxiv - Immunology 2020Quote: ... Bacteria were opsonised in heat-inactivated human serum for 30 minutes at 37°C and labelled via incubation with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Biolegend) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... sections were blocked with PBS containing 1 % bovine serum albumin (BSA) and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT ...