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Citations for BioLegend :
1 - 50 of 1133 citations for Medium Kit without Serum and with CultureBoost R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cells were washed in PBS without serum and incubated with Zombie Aqua viability dye (BioLegend) according to manufacturer’s instructions and subsequently washed with FACS buffer (PBS supplemented with 2.5% fetal bovine serum ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... with/without EpCAM-AF647 (BioLegend, clone G8.8) by retro-orbital injection 4 h before imaging ...
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bioRxiv - Biophysics 2023Quote: ... we incubate 5-6*105 NK92 cells prior to mixing them into the collagen for one hour in serum-free cell culture medium at 37°C with or without the following antibodies: 10 μg/ml anti-integrin activating β1 (CD29) antibody (Biolegend, cat. no. 303002) and the corresponding IgG1 isotype (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Serum IgE concentrations were measured using an ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... with or without OSM (500 ng/ml, BioLegend) for 24hrs at 37°C and 5% CO2 ...
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bioRxiv - Genetics 2023Quote: ... with or without OSM (500 ng/ml, BioLegend) and incubated at 37°C for 60min ...
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bioRxiv - Physiology 2022Quote: ... Organoids were harvested in cold Serum-Free Defined Medium media and incubated with Zombie (BioLegend 423107, 1:100) for 15 minutes in the dark at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were incubated in high glucose Dulbecco’s’ modified Eagle medium (DMEM) (Capricorn) supplemented with 10% fetal bovine serum (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney epithelial) cells consisted of high glucose Dulbecco’s modified Eagle medium (DMEM) (Capricorn) with 10 % fetal bovine serum (FBS) (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometry labeling (without inclusion of Feature Barcoding antibodies from BioLegend) was performed in PBS supplemented with 2% FBS ...
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bioRxiv - Immunology 2021Quote: ... with or without the addition of plate-bound αICOS (C398.4A; BioLegend) at 2 μg/μL ...
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bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: ... with or without the presence of anti-KIR antibody (1mg/ml) (Biolegend), in the presence of 2000 U/ml rhIL-2 (Peprotech ...
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bioRxiv - Physiology 2023Quote: ... with or without EPO (35U/well) (recombinant mouse EPO carrier-free, Biolegend). The ratio of splenocytes to RBCs was 1:1 ...
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bioRxiv - Immunology 2021Quote: ... Serum IL-22 was measured using LEGEND MAXTM Mouse IL-22 ELISA Kit (BioLegend, #436307) and analyzed using a SpectraMax M2 microplate reader using SoftMax Pro software (Molecular Devices).
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bioRxiv - Neuroscience 2021Quote: ... conditioned medium was collected and analyzed using mouse TNF-α ELISA kit (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Immunology 2021Quote: ... with or without adding Gal-9 mAb (10 µg/mL, Biolegend, CA, USA).) ...
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bioRxiv - Microbiology 2022Quote: Serum IFN-γ was assessed using a custom-made mouse panel 13-plex kit (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cytokines were measured in serum using the Mouse Inflammation Panel LEGENDplex cytometric bead array kit (Biolegend). Data were analysed in FlowJo and analyte concentrations were determined using a sigmoidal 4PL standard curve in Prism (GraphPad).
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bioRxiv - Immunology 2023Quote: ... IL-6 (R&D) and IL12B (Biolegend) ELISAs were preformed to manufacturers instructions
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bioRxiv - Cancer Biology 2022Quote: ... and with or without anti-IL-6 antibody (20 μg/ml, Biolegend Cat#504512). Cells were then harvested from plates ...
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bioRxiv - Microbiology 2021Quote: ... A biotinylated primary antibody was used for direct detection without signal amplification (Biolegend 339809). Positive staining was confirmed by reference to the testing information provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... with and without the CD63-APC and CD81-PE (both from BioLegend, CA, USA) antibodies ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Bioengineering 2023Quote: Serum interferon alpha and beta were analyzed by ELISA (LEGEND MAX™ Mouse IFN-β ELISA Kit – BioLegend) (ELISA MAX™ Deluxe Set Mouse IFN-α1 – BioLegend).
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bioRxiv - Immunology 2019Quote: ... recombinant murine ICAM-1 (R&D Systems, BioLegend), 4-Hydroxytamoxifen (Tocris) ...
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bioRxiv - Immunology 2023Quote: Recombinant (r) mouse cytokines were purchased from BioLegend: rIL-33 (580502) ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Pathology 2022Quote: Serum levels of IL-6 and TNFα were measured by a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).
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bioRxiv - Pathology 2021Quote: The levels of serum TNF-α were determined using the LEGEND MAX Mouse TNF-α ELISA kit (Biolegend, CA) according to the manufacturer′s instructions.
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bioRxiv - Immunology 2022Quote: ... with or without the inclusion of interleukin-4 at 20ng/mL (wt/vol, IL-4, BioLegend), interferon-g at 2.5ng/mL (wt/vol ...
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bioRxiv - Immunology 2021Quote: ... DNAM-1 (102511, R&D) or NKG2D (1D11, BioLegend) or mouse IgG1 (MOPC-21 ...
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bioRxiv - Immunology 2021Quote: ... and anti-CD268/BAFF-R-PE (7H22-E16, BioLegend). Other FACS reagents included the viability dye propidium iodide (Sigma ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... was added 1 day after serum deprivation and cell viability was assessed the following day by using Zombie Fixable Viability Kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Levels of cytokines and soluble mediators present in serum of infected mice were quantified using LEGENDplex Mouse Inflammatory Panel kit (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Genomics 2021Quote: ... Cells sorted for CTLA-4 were treated with Cell Activation Cocktail without Brefeldin A (Biolegend, Cat #423302) for 4 hours prior to fixation ...
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bioRxiv - Microbiology 2023Quote: ... 2% (v/v) Goat-serum (Biolegend) and 0.05% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... PDGFRa (Alexa 488 FAB1062G, R&D systems, APC 135908, Biolegend), Epcam (PE 118205 ...
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bioRxiv - Synthetic Biology 2021Quote: ... non-tissue culture treated plates were coated with 2μg/ml anti-CD3 clone OKT3 (NCI Preclinical Repository) with or without 2μg/ml anti-CD28 clone CD28.2 (BioLegend) by incubating at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cytokine production was quantified from cell supernatants or serum using the following ELISA kits: ELISA MAX Deluxe Set Mouse IL-10 (BioLegend, Cat# 431414), ELISA MAX Deluxe Set Human IL-10 (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... Serum IgE was assessed by ELISA (BioLegend). To deplete CD4+ T cells ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 2% Normal Serum Block (BioLegend), 1% BSA ...