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Citations for BioLegend :
601 - 650 of 823 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (Biolegend) per manufacturer’s instructions for HLA-A*02:01 ...
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bioRxiv - Immunology 2022Quote: ... The suspension of mouse CD4+ T cells and the spinal cord sections were stained with APC-Cy7-conjugated anti-mouse CD4 (clone GK1.5, Biolegend), BV510-conjugated anti-mouse CD4 (clone RM4-5 ...
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bioRxiv - Immunology 2020Quote: ... Antigen experienced CAR T cells were stained for CD69 expression using APC-conjugated anti-human CD69 antibody (Biolegend #310910). Flow cytometric data was acquired using a BD LSRFortessa with BD FACSDiva software ...
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bioRxiv - Immunology 2022Quote: ... blood leukocytes from Ldlr-/- and T-AbcdkoLdlr-/- mice were stained with a cocktail of antibodies against TCRβ-PB (Biolegend), CD8-PE ...
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bioRxiv - Genomics 2019Quote: ... CD4+ T helper cells were sorted as naive T cells (CD4+CD25−CD45RA+) or memory T cells (CD4+CD25−CD45RA−) using the following antibodies: anti-human CD45RA-PERCPCy5.5 (Clone HI100, Biolegend), anti-human CD4-APC-Cy7 (Clone OKT4 ...
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... and naïve 129/SvJae spleens were stained for regulatory T cells with anti-CD127 APC (clone SB/199, Biolegend). Cells were fixed with fix buffer (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... total T cells or naïve CD4+ T cells were isolated from mouse spleen and lymph nodes using MojoSort mouse CD3/CD4 naïve T cell Isolation Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cell were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD8+ OT-I splenic T cells were isolated from crushed spleens of naïve OT-I mice using the Mouse CD8 T cell Isolation Kit (BioLegend). Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated from the spleen by using the MojoSort Mouse Naïve CD4+ T Cell Isolation Kit (#480040, BioLegend). CD4+ T cells isolated from the OT-II spleen were cocultured with BMDCs ...
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bioRxiv - Immunology 2022Quote: ... Activation of CAR-MAIT and CAR CD8+ T cells were determined with CD25 staining using CD25-APC antibody (Biolegend). CAR expression was determined with Human Her2 / ErbB2 Protein ...
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human TCR α/β antibody (IP26: Biolegend), HLA.A2/9V-AF647 or CD19-AF647 to determine the concentration of the staining agent leading to label saturation for CMV-specific RA14 TCR T-cells ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Live naïve CD4+ T cells were purified from PBMCs post staining with fluorescence labelled antibodies to human CD3(OKT3, Biolegend), CD4 (RPA-T4 ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... CD4+ OT-II KN2 cells were purified from CD45.1.2 OT-II KN2 donor mice with the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend) and labeled with cell proliferation dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Biochemistry 2023Quote: ... Naïve CD4+ T cells were then activated with anti-CD3 (Biolegend, Cat#100340, 1:500 dilution to 5μg/mL), anti-CD28 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Neuroscience 2024Quote: ... Several positive controls were utilised: (1) polyclonal T-cell simulation with soluble αCD3 (0.5 μg/ml; clone OKT3; Biolegend) and α-CD28 (1μg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a T cell activation analysis cocktail including: anti-mouse CD3 clone 17A2 APC conjugate (BioLegend, 100235), rat anti-mouse CD4 AlexaFluor700 conjugate (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... IFN-γ secretion to validate CAR-T cell activation was measured using the Human INF-γ ELISA kit (Biolegend) or Human IFN-γ DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated for 3 days using 3 μg anti-human CD3 and CD28 antibodies (Biolegend, 300438, 377604) and 50 ng/mL of IL-2 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells (4X105/well in 96-well plate) were activated with plate-coated αCD3 (5µg/ml, Biolegend) and αCD28 (10µg/ml ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Cancer Biology 2021Quote: ... NKG2D ligands were blocked on the cancer cell lines and single cells of tumor organoids by incubating the target cells with 12 µg/mL anti-MICA/B (clone 6D4, BioLegend), 1 µg/mL anti-ULBP1 (clone 170818 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Genomics 2020Quote: ... loss of MHC class I expression in B2M knock-outs was assessed by staining with FITC-conjugated anti-HLA-A,B,C (W6/32, Biolegend). To maximize surface expression of IFNγ regulated proteins ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
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bioRxiv - Molecular Biology 2020Quote: ... MNCs were harvested by ficoll gradient centrifugation and human hematopoietic cells were identified by flow cytometry using the following antibody cocktail: HLA-A/B/C FITC (clone W6/32, Biolegend), mouse CD45.1 PE-CY7 (clone A20 ...
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Systems Biology 2022Quote: ... The population corresponding to cluster B was sorted by top 1% of CD49c abundance via red florescent antibody (Biolegend 343808). Cells corresponding to cluster A were sorted from 25-75% abundance range ...
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bioRxiv - Immunology 2021Quote: Viability of stimulated B cells were assessed by flow cytometry using FITC-labeled annexin V and propidium iodide (PI) (Biolegend). For apoptosis assays by live-imaging ...
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bioRxiv - Immunology 2020Quote: ... ABCs were isolated from purified total B cells by CD11c positive selection using a biotin conjugated CD11c antibody (301612, Biolegend) and anti-biotin magnetic beads ...
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bioRxiv - Immunology 2021Quote: ... for 15 min at RT and stained intracellularly with Fix/Perm Buffer B and antibodies for TNF (PE-Cy7, clone MAb11, BioLegend), IFN-γ (BV711 ...
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bioRxiv - Immunology 2021Quote: ... PD-1 and CD25 expression and cell viability and cell expansion were quantified by live/dead staining and the following antibodies via flow cytometry: anti-TCRa/b-PE (306708, BioLegend), anti-CD3-AF700 (11-0039-42 ...
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bioRxiv - Immunology 2021Quote: ... followed by selection of memory B cells by positive expression of CD20 and CD27 (CD20-FITC, clone 2H7; CD27-APC-Cy7, clone O323, both BioLegend) but not immunoglobulin D (IgD-PE ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then processed for single cell RNAseq as described in (Peng et al., 2019) using Total Seq B antibodies and the recommended protocol from Biolegend. Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Cat# 137024), granzyme B (QA18A28, Cat# 396413), TNF (MP6-XT22, Cat# 506329), IFN-γ (XMG1.2, Cat# 505846) were obtained from BioLegend. Anti-Foxp3 (FJK-16s ...
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bioRxiv - Immunology 2022Quote: The following antibodies were used to detect B cell populations in the BM: brilliant violet (BV) 421 anti-mouse CD19 antibody (Clone 6D5, BioLegend), PE/Cyanine5 anti-mouse/human CD45R/B220 (Clone RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... The PBMCs were fixed and permeabilized using Foxp3 / Transcription factor kit (eBioscience) and stained intracellularly with anti-Granzyme-B-Alexa-Fluor-700 (Biolegend), anti-TNF-α-efluor-450 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... and supernatants used for HLA-I IP in which purified anti-human HLA-A,B,C antibody (W6/32, BioLegend) was crosslinked to Protein G Dynabeads (Thermo Scientific ...