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Citations for BioLegend :
551 - 600 of 823 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... HTO staining was carried out with 0.5μg of β2M and CD298 antibody-conjugated oligonucleotides (Total-seq B Hastags 4, 6 and 8; BioLegend) with ∼500,000-750,000 isolated single cells ...
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Genomics 2022Quote: Single-cell RNA sequencing (scRNAseq) libraries were prepared from viably revived BM samples using hashtag B antibodies (Biolegend) and Chromium single cell 3’V3 reagent kits (10x genomics) ...
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bioRxiv - Immunology 2022Quote: ... stained with phycoerythrin (PE)-conjugated anti-HLA-A,B,C antibody for 20 minutes (BioLegend, clone W6/32), stained with Live/Dead Fixable Near-IR dye (Thermo ...
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bioRxiv - Genetics 2023Quote: ... Cells were pooled following staining with unique hashtag antibody oligonucleotide-conjugates (HTO) (30 minutes) staining (Biolegend, TotalSeq-B). Pooled samples were immediately run on a 10x Chromium Controller after preparation with a 10X Chromium 3’ library preparation kit (10X Genomics ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2019Quote: Polarized CD4+ effector T cells were generated by activating purified HD naïve CD4 T cells with plate-bound anti-CD3 (UCHT1) and anti-CD28 (CD28.2) (both 5μg/ml, Biolegend) in the presence of IL-2 (50U/ml) ...
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bioRxiv - Bioengineering 2020Quote: ... The non-macrophages cell fraction was then incubated in mouse CD4+ T cell isolation biotin-antiboy cocktail (BioLegend) and sorted ...
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bioRxiv - Microbiology 2021Quote: ... primary CD4+T cells were activated for 4 days with 1μg/ml plate-bound αCD3 antibody (cloneOKT3, Biolegend) in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were as follows: purified Armenian Hamster anti-mouse T cell receptor γ/δ (BioLegend, 118101), purified rat anti-mouse MHC class II or MHC class II Alexa 488 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2022Quote: ... Proliferation was determined by labeling T cells with the Tag-IT Violet Proliferation Cell Tracking Dye (Biolegend, #425101), according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... 5 x 105 naïve CD4+ T cells were isolated from the spleen and mesenteric lymph nodes of HDAC3FF and HDAC3ΔIEC mice via the MojoSort Mouse CD4 Naïve T Cell Isolation (BioLegend). Cell purity was confirmed by flow cytometry and T cells injected IP into age- and sex-matched Rag1-/- recipients ...
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bioRxiv - Immunology 2021Quote: Naïve CD4+ T cells were isolated from spleens and lymph nodes of BDC-2.5 transgenic NOD mice using the Mouse CD4 Naïve T Cell Isolation Kit (BioLegend). Naïve T cells were further selected positively by treatment with anti-CD62L biotinylated antibodies (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 105 CTV-labelled T-cells were stimulated with 5µg/ml of plate-bound anti-CD3 (300331, Biolegend) and 5µg/ml of soluble anti-CD28 (302913 ...
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bioRxiv - Immunology 2021Quote: ... CD3+ cell enrichment was achieved by MojoSort negative selection using MojoSort mouse CD3+ T-cell Isolation Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells were magnetically isolated from the spleen and LNs of Rag2-/- TCR7B8 Tg or TCR1A2 Tg mice on CD45.2/CD45.2 background using the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend). Then ...
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bioRxiv - Microbiology 2023Quote: ... An early activation marker of T cells was also monitored using PE-Conjugated CD-69 antibody (Biolegend, 310906). Analysis was performed on FlowJo software ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized and stained for transcription factors using the T rue-Nuclear Transcription Factor Buffer Set (Biolegend, Cat# 424401) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... transgenic T cells were labeled with Tag-it Violet Proliferation and Cell Tracking Dye (Cat No. 425101 Biolegend). The frequency of SMARTA cells that proliferated during culture was determined as described by Gett et al ...
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bioRxiv - Cancer Biology 2023Quote: BMDMs were cocultured with naïve CD4+ T cells and differentiated into Tregs in the presence 0.5 μg/mL (anti-CD3 BioLegend), 1ng/mL TGFβ (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: The T cell activation and exhaustion marker panel during tumor challenge assays: Anti-CD4 BV785 (BioLegend, Cat. # 317442), Anti-CD8 Pacific Blue (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: The T cell phenotypic characterization panel during tumor challenge assays consisted of: Anti-CD4 BV785 (BioLegend, Cat. # 317442), Anti-CD8 Pacific Blue (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: The weekly tumor challenge panel for T cell tracking consisted of: Anti-CD4 APC-Cy7 (BioLegend, Cat. # 317418), Anti-CD8 Pacific Blue (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... cells were permeabilized with 0.5% saponin for 15 min and intracellularly stained with PE-anti-perforin (clone B-D48) antibody (Biolegend) for 20 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... anti-granzyme B PE (1:50; clone QA16A02) and anti-TNFα BV711 (1:50; clone MP6-XT22) from BioLegend in permeabilization buffer at 4°C for 30 min ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... B cell purity was > 95% based on flow cytometric analysis following staining with anti-CD19 (Biolegend, San Diego, CA). FO and MZ B cells were >90% pure based on the criteria that they were sorted on.
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend). CD14+ monocytes were stimulated for 24 hours with 1ug/ml of LPS ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Immunology 2023Quote: ... This reference curve was established in each experiment for all FcyR-specific antibodies of interest (PE-conjugated clone 10.1 to detect FcyRI, clone AT10 to detect FcyRIIa/b and clone 3G8 to detect FcyRIIIa, all from Biolegend) and used to calculate receptor numbers based on fluorescence intensity of FcyR staining on CHO cells ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... about 1-2e6 mononuclear cell suspension was stained with a panel of Total-seq B antibodies (1ug/each, Biolegend) and fluorochrome conjugated flow antibodies for CD45 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β (432604) and Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville ...
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bioRxiv - Immunology 2023Quote: ... each sample was labelled with 0.1 ug of a cell hashing antibody (TotalSeq-B anti-human Hashtag antibodies 1 through 8, BioLegend). Cells were labelled for 30 minutes on ice in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... mice and cultured with M-CSF (20 µg/L, BioLegend, catalog number 576408) in vitro for 8-10 days to generate BMDMs ...
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bioRxiv - Immunology 2021Quote: ... mice were irradiated with a total of 1100 rad in two divided doses and injected intravenously with a 1:1 mixture of T cell-depleted (magnetic bead depletion; Thy1.2; BioLegend) bone marrow from CD45.1+CD45.2+ mice and control C57BL/6J (CD45.2+ ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Immunology 2022Quote: ... Stimulation of naïve CD4+ T cells was confirmed by their increased cell sizes and expression of the activation marker CD44 (BioLegend) by FACS analysis ...
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bioRxiv - Immunology 2019Quote: ... Depletion was confirmed by analysis of dLN and skin T cells stained with anti-CD4 clone RM4-4 (Biolegend), which binds a nonoverlapping epitope.
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bioRxiv - Microbiology 2021Quote: ... CD45RA+ve naïve and CD45RA-ve memory populations were further separated after CD4+ T cell isolation with CD45RA MicroBeads (Biolegend). For activated CD4+ T cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (Biolegend) per manufacturer’s instructions for HLA-A*02:01 ...
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bioRxiv - Immunology 2022Quote: ... The suspension of mouse CD4+ T cells and the spinal cord sections were stained with APC-Cy7-conjugated anti-mouse CD4 (clone GK1.5, Biolegend), BV510-conjugated anti-mouse CD4 (clone RM4-5 ...