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Citations for BioLegend :
201 - 250 of 7688 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells isolated from the mouse spleen using the MojoSort Mouse Naïve CD4 T Cell Isolation Kit (#480040, BioLegend) were stimulated in a plate coated with LEAF purified anti-mouse CD3ε antibody (clone 145-2111C ...
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bioRxiv - Cell Biology 2024Quote: ... untouched primary naïve T cells were isolated with an EasySep Mouse T cell Isolation Kit according to the manufacturer’s protocol (BioLegend). The purified CD4+ Naïve T cells were stimulated with 20 ng/mL phorbol 12-myristate13-acetate (PMA ...
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bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... cells were then fixed and permeabilized with a Transcription Factor kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2021Quote: ... and the cell lysates were assessed on a human IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Cell Biology 2023Quote: 1X Fix Concentrate from the True-Nuclear™ Transcription Factor Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 424401) was added to each sample ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected for cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801 ...
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bioRxiv - Cell Biology 2021Quote: ... MojoSort mouse CD4 T cell isolation kit was from BioLegend. Liberase TL (Research Grade ...
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bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
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bioRxiv - Cell Biology 2019Quote: ... EPO level was measured in the conditioned media using the LEGEND MAX Human Erythropoietin ELISA kit (Biolegend), according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... fixed with the True Nuclear fixation kit (BioLegend) for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... CD4+ were purified by negative selection using magnetic human CD4+ T cells nanobeads (MojoSort, Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Immunology 2021Quote: ... and donor-derived CD4 T cells were analyzed by flow cytometry following magnetic bead–enrichment with MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... CD4 T cells from the spleen and peripheral lymph nodes of C57BL/6 mice on 22-24 dpi with LCMV-c13 were harvested and enriched for CD4 T cells using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) prior to surface staining ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated using the Mojosort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, San Diego, CA) using LS columns on a QuadroMACS magnet (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2023Quote: ... CD45.1+ GFP+ OT-II CD4+ T cells were purified from mouse spleens using magnetic beads (CD4 T cell MojoSort isolation kit, Biolegend) and activated with 10μg/ml recombinant mouse IL-2 IS (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... IL-1β ELISA kit (Biolegend) was used for determination in plasma and ELISA was performed according to manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... or respective ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... and transcription factor staining kit (Biolegend). To assess the effect of RORγt inhibition on IL-17 production the specific inhibitor SR1001 (Tocris ...
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bioRxiv - Bioengineering 2020Quote: Human IFNγ was quantified via sandwich ELISA assay (430107, Biolegend) and per the manufacturer’s recommended conditions.
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bioRxiv - Microbiology 2022Quote: ... and ELISA MAX Deluxe Set Human IL-1b (Biolegend, 437004), per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend, 440804), respectively ...
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bioRxiv - Immunology 2021Quote: ... and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend, 440804), respectively ...
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bioRxiv - Immunology 2023Quote: ... ELISA MAX Deluxe Set Human IL-10 (BioLegend, Cat# 430604), ELISA MAX Deluxe Set Mouse TNF-α (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... ELISA MAX Deluxe Set Human TNF-α (BioLegend, Cat# 430204), ELISA MAX Deluxe Set Mouse IL-6 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and recombinant human transforming growth factor beta-1 (TGF-β1; Biolegend) were used at final concentrations of 10 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human T-bet-APC (clone 4B10; BioLegend); mouse anti-human GATA3-PE (clone TWAJ ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Neuroscience 2019Quote: ... Red blood cells were osmotically lysed and CD4+ T-cells were isolated using a MojoSort Mouse CD4+ T Cell Isolation Kit (Biolegend, San Diego, CA). Purified CD4s were labeled with Cell Proliferation Dye eFluor450 (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... CD4+ cells were first enriched using Mouse CD4 T Cell Isolation Kit (Biolegend) before electronic sorting ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Immunology 2024Quote: ... CORSET8 T cells were purified from the TCR Tg donor mice with the MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... Lymph node cells were harvested from TCR Tg donor mice and CD8+ T cells were purified with MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2020Quote: ... Intracellular staining was either performed using the True-Nuclear Transcription Factor Buffer Set (Biolegend, San Diego, CA, Cat # 424401) or CytoFix Fixation buffer / FACS Permeabilizing solution 2 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... The samples were incubated for 1 hour with fixation buffer from True-Nuclear™ Transcription Factor Buffer Set (Biolegend) and washed with permeabilization buffer from the same set ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed according to manufacturer’s instructions for staining intracellular antigens (eBioscience) or using the True-Nuclear Transcription Factor Buffer Set (BioLegend). Flow cytometric analysis was performed using a FACS FORTESSA and FlowJo softwares (Tree Star ...
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bioRxiv - Immunology 2021Quote: IL-21 concentration from tonsil mono-cultures was measured using Human IL-21 ELISA MAX Deluxe kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.