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Citations for BioLegend :
451 - 500 of 7688 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Immunology 2021Quote: ... Cells were activated in a 24-well plate coated with anti-CD3 (clon: 500A2, Biolegend) and soluble anti-CD28 (clon ...
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bioRxiv - Immunology 2021Quote: ELISA kits were used to measure mouse IL-17A (BioLegend, Cat# 432506) and mouse IFN-γ (BioLegend ...
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bioRxiv - Microbiology 2019Quote: IFNβ was measured with an enzyme-linked immunosorbent assay (ELISA) kit (BioLegend). Other chemokines and cytokines were measured by the MILLIPLEX mouse magnetic bead assay (Millipore ...
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bioRxiv - Immunology 2020Quote: ... IL-12p40 and IFNβ production quantified using ELISA MAX Standard kits (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and TNF levels were quantified using a mouse TNF ELISA kit (BioLegend) according to manufacturer instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Il-6 and IL-1β levels were quantified by ELISA (Biolegend kits).
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested and IL-17A was measured with ELISA kit (Biolegend). For some experiments ...
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bioRxiv - Immunology 2023Quote: ... the culture supernatants were evaluated using the ELISA MAX assay kit (BioLegend), according to the manufacturer’s specifications.
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bioRxiv - Bioengineering 2023Quote: ... Supernatants were collected and TNF-α ELISA MAX Deluxe Kit (BioLegend, UK), was used to performed ELISA according to manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and anti-human Ki-67 BUV395 (1:50 ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Microbiology 2024Quote: ... and where indicated containing 25 ng/mL human granulocyte-macrophage colony-stimulating factor (GM-CSF) ((# 572903, Biolegend, San Diego, CA) at 37 °C in a humidified chamber with 5% CO2 in 96-well ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for ELISA and cells incubated with monensin (BioLegend). Checkpoint receptors PD-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Then we resuspended the pelleted cells in 200μl Fluorescent Activated Cell Sorter (FACS) buffer [see Homogenization Buffer ingredients but dissolved in Cell Staining Buffer (Biolegend) not aCSF ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cells (106 cells/mL) were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... total T cells or naïve CD4+ T cells were isolated from mouse spleen and lymph nodes using MojoSort mouse CD3/CD4 naïve T cell Isolation Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD8+ OT-I splenic T cells were isolated from crushed spleens of naïve OT-I mice using the Mouse CD8 T cell Isolation Kit (BioLegend). Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... CD4+ OT-II KN2 cells were purified from CD45.1.2 OT-II KN2 donor mice with the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend) and labeled with cell proliferation dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... were determined by ELISA using ELISA MAX Deluxe Sets (BioLegend), and IgA concentration was measured by mouse IgA uncoated ELISA kit (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
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bioRxiv - Immunology 2022Quote: ... and Human Th LegendPlex™ kits (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... We also isolated plasma from GVHD patients and healthy donors and performed cytokine ELISAs on these plasma samples using multiplex ELISA kits (Biolegend, San Diego, CA).
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bioRxiv - Genomics 2021Quote: After the addition of 5 ul Human TruStain FcX™ (BioLegend), the cell suspension was incubated for 10 min on ice ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Cancer Biology 2022Quote: ... at 1:5 dilution and anti-human CD45-APC (BioLegend 304012) at 1:10 dilution ...
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bioRxiv - Genomics 2023Quote: ... and blocked with 5 µL Human TruStain FcX™ (422301, Biolegend) for 10 minutes at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639, Biolegend). Antibody-tagged cells were washed three times with 3 ml of Cell Staining Buffer using centrifugation speed of 500 x g for 5 minutes at 4°C ...
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bioRxiv - Genetics 2023Quote: ... we added 5 ug/mL anti-human anti-CD3 (Biolegend-317315) and 100 ng/mL phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Immunology 2020Quote: ... 116212) and T cell (PE rat anti-mouse CD4, Biolegend, 557308 ...
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bioRxiv - Genomics 2021Quote: ... T cells were stained with CD3-APC (clone HIT3a, BioLegend) antibody ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were first stained with anti-Flag (Biolegend) at 37°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were incubated with anti-Flag antibody (Biolegend) at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... and T-cell marker CD4 FITC (clone GK1.5, BioLegend, 100406) to assess purity and viability on a FACS analyzer.
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bioRxiv - Immunology 2023Quote: ... Antibodies for T cell panel were: CD4-BV605 (BioLegend, 100548), CD8a-BV650 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... T cells were then stained for CD45 (AF488, Biolegend, USA), CD4 (AF700 ...
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bioRxiv - Immunology 2024Quote: ... T cells were removed with CD90.2 antibody (30-H112, BioLegend) and Dynabeads (65601 ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated using Dynabeads M-280 Tosylactivated magnetic beads coated with αCD3 (clone OKT3, Biolegend) and either αCD28 (clone CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... Anonymous healthy donor peripheral blood mononuclear cells (PBMCs; StemCell) were activated with anti-CD3 (OKT3; Biolegend) in the presence of 100 U/mL human IL-2 ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then negatively-selected (CD8+CD44- purity > 90%) from the resulting single-cell splenocyte suspension using the MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit (BioLegend, San Diego, CA, USA). Two modifications were introduced relative to the manufacturer’s guidelines to increase purity ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were activated in 96-wells culture plates (25 x103 cells/well) coated with anti-CD3 and anti-CD28 (Biolegend) or by the addition of the DPP-8/9 inhibitor Talabostat (Val-boroPro ...
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bioRxiv - Neuroscience 2021Quote: ... conditioned medium was collected and analyzed using mouse TNF-α ELISA kit (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2019Quote: ... Supernatant was collected for IL-2 measurement using an ELISA kit (BioLegend #431801).